Dynamic changes of matrix metalloproteinase-9 in patients with Klebsiella pneumoniae meningitis

Hung Chin Tsai, Sheng Fu Liu, Kuan Sheng Wu, Yung Ching Liu, Min Hon Shi, Eng Rin Chen, Chuan Min Yen, Susan Shin Jung Lee, Yen Lin Huang, Yao Shen Chen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

To quantitate cerebrospinal fluid (CSF) concentrations of matrix metalloproteinase 9 (MMP-9) in adult patients with Klebsiella pneumoniae meningitis and to correlate levels of MMP-9 with parameters of intrathecal inflammation and analyze the kinetic changes of MMP-9. In a prospective cohort study, levels of MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP-1) concentrations were measured in the CSF of six adult patients with meningitis and 11 controls. MMP-9 and TIMP-1 were detected in all of the six patients at presentation and follow up lumbar puncture. CSF levels of MMP-9 (6.71±7.29 ng/ml) and TIMP-1(454.3±242.9 ng/ml) were higher in patients than in the control group (0.07±0.11 ng/ml and 27.14±39.34 ng/ml, respectively). Levels of MMP-9 correlated with CSF concentrations of protein, cell count and lactate. Repeated lumbar punctures showed that levels of MMP-9 decrease during clinical recovery, although the levels of MMP-9 in the CSF are variable because of the small number of cases. The relative change in gelatin zymography is comparable to the changes of MMP-9 levels found in ELISA. MMP-9 levels in CSF may be a useful tool in follow-up in patients with K. pneumoniae meningitis.

Original languageEnglish
Pages (from-to)247-253
Number of pages7
JournalInflammation
Volume31
Issue number4
DOIs
Publication statusPublished - Aug 2008
Externally publishedYes

Fingerprint

Matrix Metalloproteinase 9
Klebsiella pneumoniae
Meningitis
Cerebrospinal Fluid
Tissue Inhibitor of Metalloproteinase-1
Spinal Puncture
Cerebrospinal Fluid Proteins
Tissue Inhibitor of Metalloproteinases
Matrix Metalloproteinase 1
Matrix Metalloproteinase Inhibitors
Gelatin
Lactic Acid
Cohort Studies
Cell Count
Enzyme-Linked Immunosorbent Assay
Prospective Studies
Inflammation
Control Groups

Keywords

  • Gelatin zymography
  • Klebsiella pneumoniae
  • Matrix metalloproteinase 9
  • Meningitis

ASJC Scopus subject areas

  • Medicine(all)
  • Immunology
  • Cell Biology

Cite this

Dynamic changes of matrix metalloproteinase-9 in patients with Klebsiella pneumoniae meningitis. / Tsai, Hung Chin; Liu, Sheng Fu; Wu, Kuan Sheng; Liu, Yung Ching; Shi, Min Hon; Chen, Eng Rin; Yen, Chuan Min; Lee, Susan Shin Jung; Huang, Yen Lin; Chen, Yao Shen.

In: Inflammation, Vol. 31, No. 4, 08.2008, p. 247-253.

Research output: Contribution to journalArticle

Tsai, HC, Liu, SF, Wu, KS, Liu, YC, Shi, MH, Chen, ER, Yen, CM, Lee, SSJ, Huang, YL & Chen, YS 2008, 'Dynamic changes of matrix metalloproteinase-9 in patients with Klebsiella pneumoniae meningitis', Inflammation, vol. 31, no. 4, pp. 247-253. https://doi.org/10.1007/s10753-008-9071-5
Tsai, Hung Chin ; Liu, Sheng Fu ; Wu, Kuan Sheng ; Liu, Yung Ching ; Shi, Min Hon ; Chen, Eng Rin ; Yen, Chuan Min ; Lee, Susan Shin Jung ; Huang, Yen Lin ; Chen, Yao Shen. / Dynamic changes of matrix metalloproteinase-9 in patients with Klebsiella pneumoniae meningitis. In: Inflammation. 2008 ; Vol. 31, No. 4. pp. 247-253.
@article{ee070425a8064c84a1b9ae4c3775e814,
title = "Dynamic changes of matrix metalloproteinase-9 in patients with Klebsiella pneumoniae meningitis",
abstract = "To quantitate cerebrospinal fluid (CSF) concentrations of matrix metalloproteinase 9 (MMP-9) in adult patients with Klebsiella pneumoniae meningitis and to correlate levels of MMP-9 with parameters of intrathecal inflammation and analyze the kinetic changes of MMP-9. In a prospective cohort study, levels of MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP-1) concentrations were measured in the CSF of six adult patients with meningitis and 11 controls. MMP-9 and TIMP-1 were detected in all of the six patients at presentation and follow up lumbar puncture. CSF levels of MMP-9 (6.71±7.29 ng/ml) and TIMP-1(454.3±242.9 ng/ml) were higher in patients than in the control group (0.07±0.11 ng/ml and 27.14±39.34 ng/ml, respectively). Levels of MMP-9 correlated with CSF concentrations of protein, cell count and lactate. Repeated lumbar punctures showed that levels of MMP-9 decrease during clinical recovery, although the levels of MMP-9 in the CSF are variable because of the small number of cases. The relative change in gelatin zymography is comparable to the changes of MMP-9 levels found in ELISA. MMP-9 levels in CSF may be a useful tool in follow-up in patients with K. pneumoniae meningitis.",
keywords = "Gelatin zymography, Klebsiella pneumoniae, Matrix metalloproteinase 9, Meningitis",
author = "Tsai, {Hung Chin} and Liu, {Sheng Fu} and Wu, {Kuan Sheng} and Liu, {Yung Ching} and Shi, {Min Hon} and Chen, {Eng Rin} and Yen, {Chuan Min} and Lee, {Susan Shin Jung} and Huang, {Yen Lin} and Chen, {Yao Shen}",
year = "2008",
month = "8",
doi = "10.1007/s10753-008-9071-5",
language = "English",
volume = "31",
pages = "247--253",
journal = "Inflammation",
issn = "0360-3997",
publisher = "Springer New York",
number = "4",

}

TY - JOUR

T1 - Dynamic changes of matrix metalloproteinase-9 in patients with Klebsiella pneumoniae meningitis

AU - Tsai, Hung Chin

AU - Liu, Sheng Fu

AU - Wu, Kuan Sheng

AU - Liu, Yung Ching

AU - Shi, Min Hon

AU - Chen, Eng Rin

AU - Yen, Chuan Min

AU - Lee, Susan Shin Jung

AU - Huang, Yen Lin

AU - Chen, Yao Shen

PY - 2008/8

Y1 - 2008/8

N2 - To quantitate cerebrospinal fluid (CSF) concentrations of matrix metalloproteinase 9 (MMP-9) in adult patients with Klebsiella pneumoniae meningitis and to correlate levels of MMP-9 with parameters of intrathecal inflammation and analyze the kinetic changes of MMP-9. In a prospective cohort study, levels of MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP-1) concentrations were measured in the CSF of six adult patients with meningitis and 11 controls. MMP-9 and TIMP-1 were detected in all of the six patients at presentation and follow up lumbar puncture. CSF levels of MMP-9 (6.71±7.29 ng/ml) and TIMP-1(454.3±242.9 ng/ml) were higher in patients than in the control group (0.07±0.11 ng/ml and 27.14±39.34 ng/ml, respectively). Levels of MMP-9 correlated with CSF concentrations of protein, cell count and lactate. Repeated lumbar punctures showed that levels of MMP-9 decrease during clinical recovery, although the levels of MMP-9 in the CSF are variable because of the small number of cases. The relative change in gelatin zymography is comparable to the changes of MMP-9 levels found in ELISA. MMP-9 levels in CSF may be a useful tool in follow-up in patients with K. pneumoniae meningitis.

AB - To quantitate cerebrospinal fluid (CSF) concentrations of matrix metalloproteinase 9 (MMP-9) in adult patients with Klebsiella pneumoniae meningitis and to correlate levels of MMP-9 with parameters of intrathecal inflammation and analyze the kinetic changes of MMP-9. In a prospective cohort study, levels of MMP-9 and tissue inhibitor of matrix metalloproteinase (TIMP-1) concentrations were measured in the CSF of six adult patients with meningitis and 11 controls. MMP-9 and TIMP-1 were detected in all of the six patients at presentation and follow up lumbar puncture. CSF levels of MMP-9 (6.71±7.29 ng/ml) and TIMP-1(454.3±242.9 ng/ml) were higher in patients than in the control group (0.07±0.11 ng/ml and 27.14±39.34 ng/ml, respectively). Levels of MMP-9 correlated with CSF concentrations of protein, cell count and lactate. Repeated lumbar punctures showed that levels of MMP-9 decrease during clinical recovery, although the levels of MMP-9 in the CSF are variable because of the small number of cases. The relative change in gelatin zymography is comparable to the changes of MMP-9 levels found in ELISA. MMP-9 levels in CSF may be a useful tool in follow-up in patients with K. pneumoniae meningitis.

KW - Gelatin zymography

KW - Klebsiella pneumoniae

KW - Matrix metalloproteinase 9

KW - Meningitis

UR - http://www.scopus.com/inward/record.url?scp=47549083459&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=47549083459&partnerID=8YFLogxK

U2 - 10.1007/s10753-008-9071-5

DO - 10.1007/s10753-008-9071-5

M3 - Article

C2 - 18553165

AN - SCOPUS:47549083459

VL - 31

SP - 247

EP - 253

JO - Inflammation

JF - Inflammation

SN - 0360-3997

IS - 4

ER -