Dynamic changes in vascular permeability, cerebral blood volume, vascular density, and size after transient focal cerebral ischemia in rats: Evaluation with contrast-enhanced magnetic resonance imaging

Chien Yuan Lin, Chen Chang, Wai Mui Cheung, Ming Huang Lin, Jean Ju Chen, Chung Y. Hsu, Jyh Horng Chen, Teng Nan Lin

Research output: Contribution to journalArticle

70 Citations (Scopus)

Abstract

Postischemic cerebral blood flow and blood volume changes have been associated with angiogenesis; nevertheless, the spatiotemporal changes in vascular permeability, vascular density, and vessel size have not been investigated. Here we report a prolonged increase in vascular permeability from day 3 to day 21 after ischemia, in particular in the reperfused outer cortical layers and leptomeninges. Increased cerebral blood volume (CBV) was observed from day 3 to day 14, whereas increased blood volume in small vessels, primarily capillaries, was noticed from day 7 to day 14 in the reperfused cortex. An initial decrease in vascular density and a reciprocal increase in vessel size were observed within the reperfused cortex at days 1 and 3 after ischemia. Immunohistological analysis confirmed a similar decrease in microvessel density and an increase in vessel size in vessels with a diameter greater than 30 μm. These large-sized vessels exhibited intense basic fibroblast growth factor and endothelial nitric oxide synthase immunoreactivity, suggesting the growth of collateral vessels. By contrast, a late increase in vascular density was noticed in the reperfused outer cortex at days 14 and 21 after ischemia. Together, these findings suggest that the early phase of CBV increase is likely because of the improvement in collateral circulation, whereas the late phase of CBV increase is attributed to the surge of angiogenesis.

Original languageEnglish
Pages (from-to)1491-1501
Number of pages11
JournalJournal of Cerebral Blood Flow and Metabolism
Volume28
Issue number8
DOIs
Publication statusPublished - Aug 2008

Fingerprint

Transient Ischemic Attack
Capillary Permeability
Blood Vessels
Ischemia
Magnetic Resonance Imaging
Blood Volume
Cerebrovascular Circulation
Collateral Circulation
Nitric Oxide Synthase Type III
Fibroblast Growth Factor 2
Microvessels
Growth
Cerebral Blood Volume

Keywords

  • Angiogenesis
  • Bloodbrain barrier
  • Brain ischemia
  • MRI

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Endocrinology, Diabetes and Metabolism

Cite this

Dynamic changes in vascular permeability, cerebral blood volume, vascular density, and size after transient focal cerebral ischemia in rats : Evaluation with contrast-enhanced magnetic resonance imaging. / Lin, Chien Yuan; Chang, Chen; Cheung, Wai Mui; Lin, Ming Huang; Chen, Jean Ju; Hsu, Chung Y.; Chen, Jyh Horng; Lin, Teng Nan.

In: Journal of Cerebral Blood Flow and Metabolism, Vol. 28, No. 8, 08.2008, p. 1491-1501.

Research output: Contribution to journalArticle

Lin, Chien Yuan ; Chang, Chen ; Cheung, Wai Mui ; Lin, Ming Huang ; Chen, Jean Ju ; Hsu, Chung Y. ; Chen, Jyh Horng ; Lin, Teng Nan. / Dynamic changes in vascular permeability, cerebral blood volume, vascular density, and size after transient focal cerebral ischemia in rats : Evaluation with contrast-enhanced magnetic resonance imaging. In: Journal of Cerebral Blood Flow and Metabolism. 2008 ; Vol. 28, No. 8. pp. 1491-1501.
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