Dual-Targeting Lactoferrin-Conjugated Polymerized Magnetic Polydiacetylene-Assembled Nanocarriers with Self-Responsive Fluorescence/Magnetic Resonance Imaging for In Vivo Brain Tumor Therapy

Jen Hung Fang, Tsung Lang Chiu, Wei Chen Huang, Yen Ho Lai, Shang Hsiu Hu, You Yin Chen, San Yuan Chen

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Maintaining a high concentration of therapeutic agents in the brain is difficult due to the restrictions of the blood-brain barrier (BBB) and rapid removal from blood circulation. To enable controlled drug release and enhance the blood-brain barrier (BBB)-crossing efficiency for brain tumor therapy, a new dual-targeting magnetic polydiacetylene nanocarriers (PDNCs) delivery system modified with lactoferrin (Lf) is developed. The PDNCs are synthesized using the ultraviolet (UV) cross-linkable 10,12-pentacosadiynoic acid (PCDA) monomers through spontaneous assembling onto the surface of superparamagnetic iron oxide (SPIO) nanoparticles to form micelles-polymerized structures. The results demonstrate that PDNCs will reduce the drug leakage and further control the drug release, and display self-responsive fluorescence upon intracellular uptake for cell trafficking and imaging-guided tumor treatment. The magnetic Lf-modified PDNCs with magnetic resonance imaging (MRI) and dual-targeting ability can enhance the transportation of the PDNCs across the BBB for tracking and targeting gliomas. An enhanced therapeutic efficiency can be obtained using Lf-Cur (Curcumin)-PDNCs by improving the retention time of the encapsulated Cur and producing fourfold higher Cur amounts in the brain compared to free Cur. Animal studies also confirm that Lf targeting and controlled release act synergistically to significantly suppress tumors in orthotopic brain-bearing rats.

Original languageEnglish
Pages (from-to)688-695
Number of pages8
JournalAdvanced healthcare materials
Volume5
Issue number6
DOIs
Publication statusPublished - Mar 23 2016
Externally publishedYes

Fingerprint

Lactoferrin
Brain Neoplasms
Tumors
Brain
Fluorescence
Magnetic Resonance Imaging
Blood-Brain Barrier
Bearings (structural)
Imaging techniques
Hemodynamics
Magnetic resonance
Therapeutics
Iron oxides
Pharmaceutical Preparations
Micelles
Rats
Animals
Curcumin
Blood Circulation
Monomers

Keywords

  • Blood-brain barrier
  • Lactoferrin (Lf)
  • Magnetic nanocarriers
  • Superparamagnetic iron oxide (SPIO)

ASJC Scopus subject areas

  • Biomaterials
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

Dual-Targeting Lactoferrin-Conjugated Polymerized Magnetic Polydiacetylene-Assembled Nanocarriers with Self-Responsive Fluorescence/Magnetic Resonance Imaging for In Vivo Brain Tumor Therapy. / Fang, Jen Hung; Chiu, Tsung Lang; Huang, Wei Chen; Lai, Yen Ho; Hu, Shang Hsiu; Chen, You Yin; Chen, San Yuan.

In: Advanced healthcare materials, Vol. 5, No. 6, 23.03.2016, p. 688-695.

Research output: Contribution to journalArticle

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