Dual roles of 17-β estradiol in estrogen receptor-dependent growth inhibition in renal cell carcinoma

Kuo Chiang Chen, Chih Ming Lin, Chi Jung Huang, Shao Kuan Chen, Sheng Tang Wu, Han-Sun Chiang, Wei Chi Ku

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Background: It has been proposed that 17-β- estradiol (E2) activates estrogen receptor and inhibits renal cell carcinoma (RCC) growth. In the present study we explored the role of E2 and ER in the regulation of RCC growth. Materials and Methods: The RCC cell line ACHN was treated by E2 combining with E2 antagonist Fulvestrant or ER knockdown, and cell growth was monitored. Quantitative phosphoproteomics was applied to study the E2 regulated non-genomic phosphorylation changes. Western blotting, immunofluorescence microscopy, and apoptosis assays were used for validation. Results: E2 induced ERdependent growth inhibition in RCC cell lines. Quantitative phosphoproteomics revealed that E2 induced both apoptosis and autophagy. Cellular apoptosis was confirmed by altered.

Original languageEnglish
Pages (from-to)219-230
Number of pages12
JournalCancer Genomics and Proteomics
Volume13
Issue number3
Publication statusPublished - May 1 2016

Fingerprint

Cell growth
Renal Cell Carcinoma
Estrogen Receptors
Estradiol
Cells
Apoptosis
Growth
Phosphorylation
Cell Line
Assays
Autophagy
Microscopic examination
Fluorescence Microscopy
Western Blotting

Keywords

  • 17-β -estradiol
  • Apoptosis
  • Autophagy
  • Estrogen receptor
  • Quantitative phosphoproteomics
  • Renal cell carcinoma

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Cancer Research
  • Biochemistry

Cite this

Chen, K. C., Lin, C. M., Huang, C. J., Chen, S. K., Wu, S. T., Chiang, H-S., & Ku, W. C. (2016). Dual roles of 17-β estradiol in estrogen receptor-dependent growth inhibition in renal cell carcinoma. Cancer Genomics and Proteomics, 13(3), 219-230.

Dual roles of 17-β estradiol in estrogen receptor-dependent growth inhibition in renal cell carcinoma. / Chen, Kuo Chiang; Lin, Chih Ming; Huang, Chi Jung; Chen, Shao Kuan; Wu, Sheng Tang; Chiang, Han-Sun; Ku, Wei Chi.

In: Cancer Genomics and Proteomics, Vol. 13, No. 3, 01.05.2016, p. 219-230.

Research output: Contribution to journalArticle

Chen, KC, Lin, CM, Huang, CJ, Chen, SK, Wu, ST, Chiang, H-S & Ku, WC 2016, 'Dual roles of 17-β estradiol in estrogen receptor-dependent growth inhibition in renal cell carcinoma', Cancer Genomics and Proteomics, vol. 13, no. 3, pp. 219-230.
Chen KC, Lin CM, Huang CJ, Chen SK, Wu ST, Chiang H-S et al. Dual roles of 17-β estradiol in estrogen receptor-dependent growth inhibition in renal cell carcinoma. Cancer Genomics and Proteomics. 2016 May 1;13(3):219-230.
Chen, Kuo Chiang ; Lin, Chih Ming ; Huang, Chi Jung ; Chen, Shao Kuan ; Wu, Sheng Tang ; Chiang, Han-Sun ; Ku, Wei Chi. / Dual roles of 17-β estradiol in estrogen receptor-dependent growth inhibition in renal cell carcinoma. In: Cancer Genomics and Proteomics. 2016 ; Vol. 13, No. 3. pp. 219-230.
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