Dual-effect liposomes encapsulated with doxorubicin and chlorin e6 augment the therapeutic effect of tumor treatment

Po Chun Peng, Ruey Long Hong, Yi Jane Tsai, Pei Tzu Li, Tsuimin Tsai, Chin Tin Chen

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background and Objective: Long circulating doxorubicin (Dox)-loaded PEGylated liposomes are clinically safer than the free form due to the significant reduction of cardiac toxicity. However, the therapeutic efficacy of the PEGylated liposome could further be improved if poor diffusivity and slow drug release of the liposome in tumor interstitium can be overcome. In this study, a dual-effect liposome triggered by photodynamic effect was developed to improve the therapeutic efficacy of Dox-loaded PEGylated liposomes. Materials and Methods: Dox and chlorin e6 (Ce6) were co-encapsulated in PEGylated liposomes (named as PL-Dox-Ce6). To induce the drug release, photodynamic effect was triggered by the light irradiation of a 662nm diode laser. The cellular distribution of Dox and Ce6 was examined under confocal microscope. The in vitro and in vivo cytotoxicity of PL-Dox-Ce6 was determined via the colony formation assay and the synergistic C26 tumor model, respectively. Results: The cellular distribution of PL-Dox-Ce6 was in the cytoplasmic area; while under light irradiation, Dox was co-localized with nuclear staining positive signals. The cellular cytotoxicity of PL-Dox-Ce6 was significantly higher than the controls including liposomes encapsulating either Dox (PL-Dox) or Ce6 (PL-Ce6). The in vivo treatment efficacy of PL-Dox-Ce6 determined in the C26 tumor model reveals a significant therapeutic effect compared to that of PL-Ce6 and PL-Dox alone or in combination. Conclusion: This study indicates that this dual-effect PEGylated liposome could provide clinical advantages in the combination regimen of photodynamic therapy and chemotherapy.

Original languageEnglish
Pages (from-to)77-87
Number of pages11
JournalLasers in Surgery and Medicine
Volume47
Issue number1
DOIs
Publication statusPublished - Jan 1 2015

Fingerprint

Therapeutic Uses
Liposomes
Doxorubicin
Neoplasms
Therapeutics
chlorin e6
Light
Semiconductor Lasers
Photochemotherapy

Keywords

  • Chemotherapy
  • Photodynamic therapy
  • Photosensitizer

ASJC Scopus subject areas

  • Surgery
  • Dermatology
  • Medicine(all)

Cite this

Dual-effect liposomes encapsulated with doxorubicin and chlorin e6 augment the therapeutic effect of tumor treatment. / Peng, Po Chun; Hong, Ruey Long; Tsai, Yi Jane; Li, Pei Tzu; Tsai, Tsuimin; Chen, Chin Tin.

In: Lasers in Surgery and Medicine, Vol. 47, No. 1, 01.01.2015, p. 77-87.

Research output: Contribution to journalArticle

Peng, Po Chun ; Hong, Ruey Long ; Tsai, Yi Jane ; Li, Pei Tzu ; Tsai, Tsuimin ; Chen, Chin Tin. / Dual-effect liposomes encapsulated with doxorubicin and chlorin e6 augment the therapeutic effect of tumor treatment. In: Lasers in Surgery and Medicine. 2015 ; Vol. 47, No. 1. pp. 77-87.
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abstract = "Background and Objective: Long circulating doxorubicin (Dox)-loaded PEGylated liposomes are clinically safer than the free form due to the significant reduction of cardiac toxicity. However, the therapeutic efficacy of the PEGylated liposome could further be improved if poor diffusivity and slow drug release of the liposome in tumor interstitium can be overcome. In this study, a dual-effect liposome triggered by photodynamic effect was developed to improve the therapeutic efficacy of Dox-loaded PEGylated liposomes. Materials and Methods: Dox and chlorin e6 (Ce6) were co-encapsulated in PEGylated liposomes (named as PL-Dox-Ce6). To induce the drug release, photodynamic effect was triggered by the light irradiation of a 662nm diode laser. The cellular distribution of Dox and Ce6 was examined under confocal microscope. The in vitro and in vivo cytotoxicity of PL-Dox-Ce6 was determined via the colony formation assay and the synergistic C26 tumor model, respectively. Results: The cellular distribution of PL-Dox-Ce6 was in the cytoplasmic area; while under light irradiation, Dox was co-localized with nuclear staining positive signals. The cellular cytotoxicity of PL-Dox-Ce6 was significantly higher than the controls including liposomes encapsulating either Dox (PL-Dox) or Ce6 (PL-Ce6). The in vivo treatment efficacy of PL-Dox-Ce6 determined in the C26 tumor model reveals a significant therapeutic effect compared to that of PL-Ce6 and PL-Dox alone or in combination. Conclusion: This study indicates that this dual-effect PEGylated liposome could provide clinical advantages in the combination regimen of photodynamic therapy and chemotherapy.",
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