Dual-compartmental transcriptomic + proteomic analysis of a marine endosymbiosis exposed to environmental change

Anderson B. Mayfield, Yu Bin Wang, Chii Shiarng Chen, Shu Hwa Chen, Chung Yen Lin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

As significant anthropogenic pressures are putting undue stress on the world's oceans, there has been a concerted effort to understand how marine organisms respond to environmental change. Transcriptomic approaches, in particular, have been readily employed to document the mRNA-level response of a plethora of marine invertebrates exposed to an array of simulated stress scenarios, with the tacit and untested assumption being that the respective proteins show a corresponding trend. To better understand the degree of congruency between mRNA and protein expression in an endosymbiotic marine invertebrate, mRNAs and proteins were sequenced from the same samples of the common, Indo-Pacific coral Seriatopora hystrix exposed to stable or upwelling-simulating conditions for 1 week. Of the 167 proteins downregulated at variable temperature, only two were associated with mRNAs that were also differentially expressed between treatments. Of the 378 differentially expressed genes, none were associated with a differentially expressed protein. Collectively, these results highlight the inherent risk of inferring cellular behaviour based on mRNA expression data alone and challenge the current, mRNA-focused approach taken by most marine and many molecular biologists.

Original languageEnglish
Pages (from-to)5944-5958
Number of pages15
JournalMolecular Ecology
Volume25
Issue number23
DOIs
Publication statusPublished - Dec 1 2016
Externally publishedYes

Fingerprint

Symbiosis
proteomics
transcriptomics
Proteomics
symbiosis
environmental change
Messenger RNA
protein
proteins
invertebrates
Invertebrates
Proteins
invertebrate
Porcupines
biologists
corals
Aquatic Organisms
Anthozoa
protein synthesis
oceans

Keywords

  • acclimation
  • coral reef
  • endosymbiosis
  • environmental change
  • gene expression
  • proteome
  • transcriptome

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Genetics

Cite this

Dual-compartmental transcriptomic + proteomic analysis of a marine endosymbiosis exposed to environmental change. / Mayfield, Anderson B.; Wang, Yu Bin; Chen, Chii Shiarng; Chen, Shu Hwa; Lin, Chung Yen.

In: Molecular Ecology, Vol. 25, No. 23, 01.12.2016, p. 5944-5958.

Research output: Contribution to journalArticle

Mayfield, Anderson B. ; Wang, Yu Bin ; Chen, Chii Shiarng ; Chen, Shu Hwa ; Lin, Chung Yen. / Dual-compartmental transcriptomic + proteomic analysis of a marine endosymbiosis exposed to environmental change. In: Molecular Ecology. 2016 ; Vol. 25, No. 23. pp. 5944-5958.
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