Drug release from chitosan-alginate complex beads reinforced by a naturally occurring cross-linking agent

Fwu Long Mi, Hsing Wen Sung, Shin Shing Shyu

Research output: Contribution to journalArticle

244 Citations (Scopus)

Abstract

Cross-linking reinforced chitosan-alginate complex beads were prepared by dropping chitosan droplets into alginate-cross-linker co-existing gelling solution. A novel, naturally occurring material - genipin was used as the cross-linking agent. Genipin and its related iridoid compounds, extracted from the Gardenia fruits, have been used in the traditional Chinese medicine for the treatments of jaundice and various inflammatory and hepatic diseases. The gelation of chitosan droplets in alginate-genipin mixed solution depends on both controlling factors: chitosan-alginate polyelectrolyte complex and chitosan cross-linked by genipin. The reaction of chitosan-alginate complex dominates the formation of skin layer on the surface of beads, but cross-linking of chitosan by genipin dominates the curing of inner core of beads. The swelling ratio of the cross-linking reinforced chitosan-alginate complex bead decreases as the pH or concentration of alginate in the gelling solution was decreased. The protonation of amine group of chitosan by hydrogen ions from acid or the shield of charge of ammonium group of chitosan by Cl- ions result in the decrease of cross-linking density due to the inhibition of nucleophilic attack on the dihydropyran ring of genipin. Contrary to the swelling properties, the rate of indomethacin releasing out of the chitosan-alginate complex beads increases with the decrease in pH or concentration of alginate in the gelling solution due to the decreased cross-linking density of the beads. The cytotoxic examination suggested that the chitosan-alginate beads cross-linked by genipin had a good cellular compatibility.

Original languageEnglish
Pages (from-to)61-72
Number of pages12
JournalCarbohydrate Polymers
Volume48
Issue number1
DOIs
Publication statusPublished - Apr 1 2002
Externally publishedYes

Fingerprint

Alginate
Chitosan
alginates
chitosan
drugs
Pharmaceutical Preparations
crosslinking
gelation
droplets
Swelling
cross-linking reagents
alginic acid
Drug Liberation
Gardenia
hydrogen ions
jaundice
indomethacin
Protonation
Chinese Traditional Medicine
Ions

Keywords

  • Alginate
  • Chitosan
  • Genipin
  • Polyelectrolyte complex
  • Reinforce

ASJC Scopus subject areas

  • Food Science
  • Biochemistry

Cite this

Drug release from chitosan-alginate complex beads reinforced by a naturally occurring cross-linking agent. / Mi, Fwu Long; Sung, Hsing Wen; Shyu, Shin Shing.

In: Carbohydrate Polymers, Vol. 48, No. 1, 01.04.2002, p. 61-72.

Research output: Contribution to journalArticle

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