Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis

Kai Wen Hsu, An Ming Wang, Yueh Hsin Ping, Kuo Hung Huang, Tzu Ting Huang, Hsin Chen Lee, Su Shun Lo, Chin Wen Chi, Tian-Shun Tsai

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Gastric carcinoma is one of the most common malignancies and the second most lethal cancer worldwide. The mechanisms underlying aggressiveness of gastric cancer still remain obscure. c-Myc promoter binding protein 1 (MBP-1) is a negative regulator of c-myc expression and ubiquitously expressed in normal human tissues. It is produced by alternative translation initiation of α-enolase gene. Both MBP-1 and α-enolase are involved in the control of tumorigenesis including gastric cancer. MicroRNAs (miRNAs) are involved in tumorigenesis and could have diagnostic, prognostic and therapeutic potential. In this study, whether miRNAs modulate tumorigenesis of gastric cancer cells through targeting MBP-1 was evaluated. We found that miR-363 targets 3′-untranslated region of human MBP-1/α-enolase messenger RNA. The exogenous miR-363 promotes growth, viability, progression, epithelial-mesenchymal transition and tumorsphere formation of SC-M1 gastric cancer cells through downregulation of MBP-1, whereas the knockdown of endogenous miR-363 suppresses tumorigenesis and progression of SC-M1 cells via upregulation of MBP-1. The miR-363/MBP-1 axis is also involved in the control of carcinogenesis in KATO III and SNU-16 gastric cancer cells. Furthermore, miR-363 induces the xenografted tumor growth and lung metastasis of SC-M1 cells through MBP-1 in vivo. Taken together, these results suggest that miR-363 plays an important role in the increment of gastric carcinogenesis via targeting MBP-1.

Original languageEnglish
Pages (from-to)208-217
Number of pages10
JournalCarcinogenesis
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 2014

Fingerprint

MicroRNAs
Stomach
Carrier Proteins
Carcinogenesis
Down-Regulation
Stomach Neoplasms
Neoplasms
Phosphopyruvate Hydratase
Epithelial-Mesenchymal Transition
Second Primary Neoplasms
3' Untranslated Regions
Growth
Up-Regulation
Neoplasm Metastasis
Carcinoma
Lung
Messenger RNA
Genes

ASJC Scopus subject areas

  • Cancer Research

Cite this

Hsu, K. W., Wang, A. M., Ping, Y. H., Huang, K. H., Huang, T. T., Lee, H. C., ... Tsai, T-S. (2014). Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis. Carcinogenesis, 35(1), 208-217. https://doi.org/10.1093/carcin/bgt285

Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis. / Hsu, Kai Wen; Wang, An Ming; Ping, Yueh Hsin; Huang, Kuo Hung; Huang, Tzu Ting; Lee, Hsin Chen; Lo, Su Shun; Chi, Chin Wen; Tsai, Tian-Shun.

In: Carcinogenesis, Vol. 35, No. 1, 01.2014, p. 208-217.

Research output: Contribution to journalArticle

Hsu, KW, Wang, AM, Ping, YH, Huang, KH, Huang, TT, Lee, HC, Lo, SS, Chi, CW & Tsai, T-S 2014, 'Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis', Carcinogenesis, vol. 35, no. 1, pp. 208-217. https://doi.org/10.1093/carcin/bgt285
Hsu, Kai Wen ; Wang, An Ming ; Ping, Yueh Hsin ; Huang, Kuo Hung ; Huang, Tzu Ting ; Lee, Hsin Chen ; Lo, Su Shun ; Chi, Chin Wen ; Tsai, Tian-Shun. / Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis. In: Carcinogenesis. 2014 ; Vol. 35, No. 1. pp. 208-217.
@article{c886ed91c8a24844beef5278a9b598d6,
title = "Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis",
abstract = "Gastric carcinoma is one of the most common malignancies and the second most lethal cancer worldwide. The mechanisms underlying aggressiveness of gastric cancer still remain obscure. c-Myc promoter binding protein 1 (MBP-1) is a negative regulator of c-myc expression and ubiquitously expressed in normal human tissues. It is produced by alternative translation initiation of α-enolase gene. Both MBP-1 and α-enolase are involved in the control of tumorigenesis including gastric cancer. MicroRNAs (miRNAs) are involved in tumorigenesis and could have diagnostic, prognostic and therapeutic potential. In this study, whether miRNAs modulate tumorigenesis of gastric cancer cells through targeting MBP-1 was evaluated. We found that miR-363 targets 3′-untranslated region of human MBP-1/α-enolase messenger RNA. The exogenous miR-363 promotes growth, viability, progression, epithelial-mesenchymal transition and tumorsphere formation of SC-M1 gastric cancer cells through downregulation of MBP-1, whereas the knockdown of endogenous miR-363 suppresses tumorigenesis and progression of SC-M1 cells via upregulation of MBP-1. The miR-363/MBP-1 axis is also involved in the control of carcinogenesis in KATO III and SNU-16 gastric cancer cells. Furthermore, miR-363 induces the xenografted tumor growth and lung metastasis of SC-M1 cells through MBP-1 in vivo. Taken together, these results suggest that miR-363 plays an important role in the increment of gastric carcinogenesis via targeting MBP-1.",
author = "Hsu, {Kai Wen} and Wang, {An Ming} and Ping, {Yueh Hsin} and Huang, {Kuo Hung} and Huang, {Tzu Ting} and Lee, {Hsin Chen} and Lo, {Su Shun} and Chi, {Chin Wen} and Tian-Shun Tsai",
year = "2014",
month = "1",
doi = "10.1093/carcin/bgt285",
language = "English",
volume = "35",
pages = "208--217",
journal = "Carcinogenesis",
issn = "0143-3334",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Downregulation of tumor suppressor MBP-1 by microRNA-363 in gastric carcinogenesis

AU - Hsu, Kai Wen

AU - Wang, An Ming

AU - Ping, Yueh Hsin

AU - Huang, Kuo Hung

AU - Huang, Tzu Ting

AU - Lee, Hsin Chen

AU - Lo, Su Shun

AU - Chi, Chin Wen

AU - Tsai, Tian-Shun

PY - 2014/1

Y1 - 2014/1

N2 - Gastric carcinoma is one of the most common malignancies and the second most lethal cancer worldwide. The mechanisms underlying aggressiveness of gastric cancer still remain obscure. c-Myc promoter binding protein 1 (MBP-1) is a negative regulator of c-myc expression and ubiquitously expressed in normal human tissues. It is produced by alternative translation initiation of α-enolase gene. Both MBP-1 and α-enolase are involved in the control of tumorigenesis including gastric cancer. MicroRNAs (miRNAs) are involved in tumorigenesis and could have diagnostic, prognostic and therapeutic potential. In this study, whether miRNAs modulate tumorigenesis of gastric cancer cells through targeting MBP-1 was evaluated. We found that miR-363 targets 3′-untranslated region of human MBP-1/α-enolase messenger RNA. The exogenous miR-363 promotes growth, viability, progression, epithelial-mesenchymal transition and tumorsphere formation of SC-M1 gastric cancer cells through downregulation of MBP-1, whereas the knockdown of endogenous miR-363 suppresses tumorigenesis and progression of SC-M1 cells via upregulation of MBP-1. The miR-363/MBP-1 axis is also involved in the control of carcinogenesis in KATO III and SNU-16 gastric cancer cells. Furthermore, miR-363 induces the xenografted tumor growth and lung metastasis of SC-M1 cells through MBP-1 in vivo. Taken together, these results suggest that miR-363 plays an important role in the increment of gastric carcinogenesis via targeting MBP-1.

AB - Gastric carcinoma is one of the most common malignancies and the second most lethal cancer worldwide. The mechanisms underlying aggressiveness of gastric cancer still remain obscure. c-Myc promoter binding protein 1 (MBP-1) is a negative regulator of c-myc expression and ubiquitously expressed in normal human tissues. It is produced by alternative translation initiation of α-enolase gene. Both MBP-1 and α-enolase are involved in the control of tumorigenesis including gastric cancer. MicroRNAs (miRNAs) are involved in tumorigenesis and could have diagnostic, prognostic and therapeutic potential. In this study, whether miRNAs modulate tumorigenesis of gastric cancer cells through targeting MBP-1 was evaluated. We found that miR-363 targets 3′-untranslated region of human MBP-1/α-enolase messenger RNA. The exogenous miR-363 promotes growth, viability, progression, epithelial-mesenchymal transition and tumorsphere formation of SC-M1 gastric cancer cells through downregulation of MBP-1, whereas the knockdown of endogenous miR-363 suppresses tumorigenesis and progression of SC-M1 cells via upregulation of MBP-1. The miR-363/MBP-1 axis is also involved in the control of carcinogenesis in KATO III and SNU-16 gastric cancer cells. Furthermore, miR-363 induces the xenografted tumor growth and lung metastasis of SC-M1 cells through MBP-1 in vivo. Taken together, these results suggest that miR-363 plays an important role in the increment of gastric carcinogenesis via targeting MBP-1.

UR - http://www.scopus.com/inward/record.url?scp=84891358116&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84891358116&partnerID=8YFLogxK

U2 - 10.1093/carcin/bgt285

DO - 10.1093/carcin/bgt285

M3 - Article

C2 - 23975832

AN - SCOPUS:84891358116

VL - 35

SP - 208

EP - 217

JO - Carcinogenesis

JF - Carcinogenesis

SN - 0143-3334

IS - 1

ER -