Downregulation of superoxide dismutase activity and gene expression in cultured rat brain astrocytes after incubation with vitamin C

Pai Feng Kao, Wen Sen Lee, Ju Chi Liu, Paul Chan, Jen Chen Tsai, Yung Ho Hsu, Wen Yin Chang, Tzu-Hurng Cheng, Shue Sen Liao

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Reactive oxygen species have been linked with neuropathological changes in the central nervous system. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Superoxide dismutase (SOD) is an endogenous enzyme which can scavenge reactive oxygen species. This study investigated the effect of supplementation with ascorbic acid (vitamin C) on the changes of SOD in cultured neurological cells. Rat brain astrocytes (RBA-1 cells) were incubated with vitamin C and divided into four groups: a control group (without vitamin C) and three treatment groups with vitamin C at 40, 80, and 160 μmol/l. After short-term (2 days) and long-term (7 days) incubation, SOD activity, SOD mRNA level by Northern blotting, and SOD protein amounts by Western blotting were measured. After 2 days of incubation, vitamin C resulted in a decrease in the activity of SOD in a concentration-dependent manner (Mn-SOD from 14.8 ± 1.2 to 13.2 ± 0.5 U/mg protein and Cu/Zn-SOD from 64.8 ± 1.2 to 51.7 ± 0.9 U/mg protein; p <0.05), and vitamin C also attenuated the Cu/Zn-SOD mRNA level from 100 to 86.3 ± 6.7%; p <0.01), whereas the protein amounts of these two SODs remained unchanged. After 7 days of incubation with vitamin C, the SOD activity of RBA-1 cells decreased significantly (Mn-SOD from 14.9 ± 0.3 to 11.8 ± 0.3 U/mg protein and Cu/Zn SOD from 61.8 ± 1.8 to 54.6 ± 0.9 U/mg protein; p <0.01), and the mRNA level was also attenuated (Mn-SOD from 100 to 86.8 ± 8.7% and Cu/Zn-SOD from 100 to 84.7 ± 4.8%; p <0.01). These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may downregulate activity and gene expression of SOD in cultured RBA-1 cells.

Original languageEnglish
Pages (from-to)1-6
Number of pages6
JournalPharmacology
Volume69
Issue number1
DOIs
Publication statusPublished - 2003

Fingerprint

Astrocytes
Ascorbic Acid
Superoxide Dismutase
Down-Regulation
Gene Expression
Brain
Proteins
Messenger RNA
Reactive Oxygen Species
Northern Blotting
Epidemiologic Studies
Cultured Cells
Central Nervous System
Antioxidants
Western Blotting
Control Groups
Superoxide Dismutase-1
Enzymes

Keywords

  • Rat brain astrocytes
  • Reactive oxygen species
  • Superoxide dismutase
  • Vitamin C

ASJC Scopus subject areas

  • Pharmacology

Cite this

Downregulation of superoxide dismutase activity and gene expression in cultured rat brain astrocytes after incubation with vitamin C. / Kao, Pai Feng; Lee, Wen Sen; Liu, Ju Chi; Chan, Paul; Tsai, Jen Chen; Hsu, Yung Ho; Chang, Wen Yin; Cheng, Tzu-Hurng; Liao, Shue Sen.

In: Pharmacology, Vol. 69, No. 1, 2003, p. 1-6.

Research output: Contribution to journalArticle

@article{0e944bb039e746b2977cebfbaf044e04,
title = "Downregulation of superoxide dismutase activity and gene expression in cultured rat brain astrocytes after incubation with vitamin C",
abstract = "Reactive oxygen species have been linked with neuropathological changes in the central nervous system. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Superoxide dismutase (SOD) is an endogenous enzyme which can scavenge reactive oxygen species. This study investigated the effect of supplementation with ascorbic acid (vitamin C) on the changes of SOD in cultured neurological cells. Rat brain astrocytes (RBA-1 cells) were incubated with vitamin C and divided into four groups: a control group (without vitamin C) and three treatment groups with vitamin C at 40, 80, and 160 μmol/l. After short-term (2 days) and long-term (7 days) incubation, SOD activity, SOD mRNA level by Northern blotting, and SOD protein amounts by Western blotting were measured. After 2 days of incubation, vitamin C resulted in a decrease in the activity of SOD in a concentration-dependent manner (Mn-SOD from 14.8 ± 1.2 to 13.2 ± 0.5 U/mg protein and Cu/Zn-SOD from 64.8 ± 1.2 to 51.7 ± 0.9 U/mg protein; p <0.05), and vitamin C also attenuated the Cu/Zn-SOD mRNA level from 100 to 86.3 ± 6.7{\%}; p <0.01), whereas the protein amounts of these two SODs remained unchanged. After 7 days of incubation with vitamin C, the SOD activity of RBA-1 cells decreased significantly (Mn-SOD from 14.9 ± 0.3 to 11.8 ± 0.3 U/mg protein and Cu/Zn SOD from 61.8 ± 1.8 to 54.6 ± 0.9 U/mg protein; p <0.01), and the mRNA level was also attenuated (Mn-SOD from 100 to 86.8 ± 8.7{\%} and Cu/Zn-SOD from 100 to 84.7 ± 4.8{\%}; p <0.01). These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may downregulate activity and gene expression of SOD in cultured RBA-1 cells.",
keywords = "Rat brain astrocytes, Reactive oxygen species, Superoxide dismutase, Vitamin C",
author = "Kao, {Pai Feng} and Lee, {Wen Sen} and Liu, {Ju Chi} and Paul Chan and Tsai, {Jen Chen} and Hsu, {Yung Ho} and Chang, {Wen Yin} and Tzu-Hurng Cheng and Liao, {Shue Sen}",
year = "2003",
doi = "10.1159/000071242",
language = "English",
volume = "69",
pages = "1--6",
journal = "Pharmacology",
issn = "0031-7012",
publisher = "S. Karger AG",
number = "1",

}

TY - JOUR

T1 - Downregulation of superoxide dismutase activity and gene expression in cultured rat brain astrocytes after incubation with vitamin C

AU - Kao, Pai Feng

AU - Lee, Wen Sen

AU - Liu, Ju Chi

AU - Chan, Paul

AU - Tsai, Jen Chen

AU - Hsu, Yung Ho

AU - Chang, Wen Yin

AU - Cheng, Tzu-Hurng

AU - Liao, Shue Sen

PY - 2003

Y1 - 2003

N2 - Reactive oxygen species have been linked with neuropathological changes in the central nervous system. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Superoxide dismutase (SOD) is an endogenous enzyme which can scavenge reactive oxygen species. This study investigated the effect of supplementation with ascorbic acid (vitamin C) on the changes of SOD in cultured neurological cells. Rat brain astrocytes (RBA-1 cells) were incubated with vitamin C and divided into four groups: a control group (without vitamin C) and three treatment groups with vitamin C at 40, 80, and 160 μmol/l. After short-term (2 days) and long-term (7 days) incubation, SOD activity, SOD mRNA level by Northern blotting, and SOD protein amounts by Western blotting were measured. After 2 days of incubation, vitamin C resulted in a decrease in the activity of SOD in a concentration-dependent manner (Mn-SOD from 14.8 ± 1.2 to 13.2 ± 0.5 U/mg protein and Cu/Zn-SOD from 64.8 ± 1.2 to 51.7 ± 0.9 U/mg protein; p <0.05), and vitamin C also attenuated the Cu/Zn-SOD mRNA level from 100 to 86.3 ± 6.7%; p <0.01), whereas the protein amounts of these two SODs remained unchanged. After 7 days of incubation with vitamin C, the SOD activity of RBA-1 cells decreased significantly (Mn-SOD from 14.9 ± 0.3 to 11.8 ± 0.3 U/mg protein and Cu/Zn SOD from 61.8 ± 1.8 to 54.6 ± 0.9 U/mg protein; p <0.01), and the mRNA level was also attenuated (Mn-SOD from 100 to 86.8 ± 8.7% and Cu/Zn-SOD from 100 to 84.7 ± 4.8%; p <0.01). These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may downregulate activity and gene expression of SOD in cultured RBA-1 cells.

AB - Reactive oxygen species have been linked with neuropathological changes in the central nervous system. Epidemiological studies supported the beneficial effect of supplementation of antioxidants. Superoxide dismutase (SOD) is an endogenous enzyme which can scavenge reactive oxygen species. This study investigated the effect of supplementation with ascorbic acid (vitamin C) on the changes of SOD in cultured neurological cells. Rat brain astrocytes (RBA-1 cells) were incubated with vitamin C and divided into four groups: a control group (without vitamin C) and three treatment groups with vitamin C at 40, 80, and 160 μmol/l. After short-term (2 days) and long-term (7 days) incubation, SOD activity, SOD mRNA level by Northern blotting, and SOD protein amounts by Western blotting were measured. After 2 days of incubation, vitamin C resulted in a decrease in the activity of SOD in a concentration-dependent manner (Mn-SOD from 14.8 ± 1.2 to 13.2 ± 0.5 U/mg protein and Cu/Zn-SOD from 64.8 ± 1.2 to 51.7 ± 0.9 U/mg protein; p <0.05), and vitamin C also attenuated the Cu/Zn-SOD mRNA level from 100 to 86.3 ± 6.7%; p <0.01), whereas the protein amounts of these two SODs remained unchanged. After 7 days of incubation with vitamin C, the SOD activity of RBA-1 cells decreased significantly (Mn-SOD from 14.9 ± 0.3 to 11.8 ± 0.3 U/mg protein and Cu/Zn SOD from 61.8 ± 1.8 to 54.6 ± 0.9 U/mg protein; p <0.01), and the mRNA level was also attenuated (Mn-SOD from 100 to 86.8 ± 8.7% and Cu/Zn-SOD from 100 to 84.7 ± 4.8%; p <0.01). These results suggest that 2 and 7 days of incubation with relatively high concentrations of vitamin C may downregulate activity and gene expression of SOD in cultured RBA-1 cells.

KW - Rat brain astrocytes

KW - Reactive oxygen species

KW - Superoxide dismutase

KW - Vitamin C

UR - http://www.scopus.com/inward/record.url?scp=0043175437&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0043175437&partnerID=8YFLogxK

U2 - 10.1159/000071242

DO - 10.1159/000071242

M3 - Article

VL - 69

SP - 1

EP - 6

JO - Pharmacology

JF - Pharmacology

SN - 0031-7012

IS - 1

ER -