Downregulation of phospholipase C δ3 by cAMP and calcium

Fu Gong Lin, Hwei Fang Cheng, I. Fang Lee, Hsiao Jung Kao, Shih Hurng Loh, Wei H. Lee

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Four different isoforms of mammalian phospholipase C δ (PLCδ) have been described. PLCδ1, the best-understood isoform, is activated by an atypical GTP-binding protein. It has been suggested that it is a calcium signal amplifier. However, very less is known about other subtypes, including PLCδ3. Therefore, in the present study, we examined the expression of PLCδ3 in different human tissues. Moreover, the cellular underlying regulation for PLCδ3 was studied in different cell lines. Our study showed that the mRNA and protein levels differed significantly among human tissues. The human PLCδ3 gene was composed of 15 exons and 1 putative cAMP response element in the 5′-end promoter region. PLCδ3 mRNA expression was downregulated by cAMP and calcium in both the human normal embryonic lung tissue diploid WI38 cell line and the glioblastoma/astrocytoma U373 cell line. However, mRNA expression showed no impact by PKC activators or inhibitors. This study shows the human PLCδ3 expression pattern and is the first report that PLCδ3 gene expression is downregulation by cAMP and calcium.

Original languageEnglish
Pages (from-to)274-280
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume286
Issue number2
DOIs
Publication statusPublished - 2001
Externally publishedYes

Fingerprint

Type C Phospholipases
Down-Regulation
Cells
Tissue
Calcium
Messenger RNA
Protein Isoforms
Cell Line
Response Elements
GTP-Binding Proteins
Genetic Promoter Regions
Gene expression
Exons
Genes
Astrocytoma
Glioblastoma
Diploidy
Proteins
Gene Expression
Lung

Keywords

  • Calcium
  • cAMP
  • mRNA
  • PKC
  • PLCδ3

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Downregulation of phospholipase C δ3 by cAMP and calcium. / Lin, Fu Gong; Cheng, Hwei Fang; Lee, I. Fang; Kao, Hsiao Jung; Loh, Shih Hurng; Lee, Wei H.

In: Biochemical and Biophysical Research Communications, Vol. 286, No. 2, 2001, p. 274-280.

Research output: Contribution to journalArticle

Lin, Fu Gong ; Cheng, Hwei Fang ; Lee, I. Fang ; Kao, Hsiao Jung ; Loh, Shih Hurng ; Lee, Wei H. / Downregulation of phospholipase C δ3 by cAMP and calcium. In: Biochemical and Biophysical Research Communications. 2001 ; Vol. 286, No. 2. pp. 274-280.
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