Abstract
Background This study was undertaken to identify the genes in response to areca nut extract, a potential carcinogen of oral cancer. Methods Two oral cancer sublines chronically treated with areca nut extract were established. Methods such as microarray and immunohistochemistry were used to screen and validate the genes' altered expressions in areca nut extract-sublines or in cancer tissues. Results A total of 35 genes were differentially expressed in both sublines. Several functional pathways were significantly altered. Six genes were confirmed over 2-fold of changes, including Ches1. Functional analyses showed that overexpression of Ches1 suppressed cell growth and arrested cells in the G2/M phase. Consistently, this gene has reduced expression in 52% of oral cancer tissues, which was significantly correlated with the areca nut chewing habit of patients (p =.04). Conclusion We identified 35 candidates and validated 6 genes that may be associated with areca nut-induced oral cancer. Loss of Ches1 may be attributed to areca nut extract-induced oral carcinogenesis.
| Original language | English |
|---|---|
| Pages (from-to) | 257-266 |
| Number of pages | 10 |
| Journal | Head and Neck |
| Volume | 33 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - Feb 2011 |
| Externally published | Yes |
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Keywords
- areca nut
- Ches1
- clinical association
- growth suppression
- oral cancer
ASJC Scopus subject areas
- Otorhinolaryngology
Cite this
Downregulation of Ches1 and other novel genes in oral cancer cells chronically exposed to areca nut extract. / Chen, Yin Ju; Liao, Chun Ta; Chen, Po Jen; Lee, Li Yu; Li, Yi Chen; Chen, I. How; Wang, Hung Ming; Chang, Joseph T.; Chen, Li Ju; Yen, Tzu Chen; Tang, Chuan Yi; Cheng, Ann Joy.
In: Head and Neck, Vol. 33, No. 2, 02.2011, p. 257-266.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Downregulation of Ches1 and other novel genes in oral cancer cells chronically exposed to areca nut extract
AU - Chen, Yin Ju
AU - Liao, Chun Ta
AU - Chen, Po Jen
AU - Lee, Li Yu
AU - Li, Yi Chen
AU - Chen, I. How
AU - Wang, Hung Ming
AU - Chang, Joseph T.
AU - Chen, Li Ju
AU - Yen, Tzu Chen
AU - Tang, Chuan Yi
AU - Cheng, Ann Joy
PY - 2011/2
Y1 - 2011/2
N2 - Background This study was undertaken to identify the genes in response to areca nut extract, a potential carcinogen of oral cancer. Methods Two oral cancer sublines chronically treated with areca nut extract were established. Methods such as microarray and immunohistochemistry were used to screen and validate the genes' altered expressions in areca nut extract-sublines or in cancer tissues. Results A total of 35 genes were differentially expressed in both sublines. Several functional pathways were significantly altered. Six genes were confirmed over 2-fold of changes, including Ches1. Functional analyses showed that overexpression of Ches1 suppressed cell growth and arrested cells in the G2/M phase. Consistently, this gene has reduced expression in 52% of oral cancer tissues, which was significantly correlated with the areca nut chewing habit of patients (p =.04). Conclusion We identified 35 candidates and validated 6 genes that may be associated with areca nut-induced oral cancer. Loss of Ches1 may be attributed to areca nut extract-induced oral carcinogenesis.
AB - Background This study was undertaken to identify the genes in response to areca nut extract, a potential carcinogen of oral cancer. Methods Two oral cancer sublines chronically treated with areca nut extract were established. Methods such as microarray and immunohistochemistry were used to screen and validate the genes' altered expressions in areca nut extract-sublines or in cancer tissues. Results A total of 35 genes were differentially expressed in both sublines. Several functional pathways were significantly altered. Six genes were confirmed over 2-fold of changes, including Ches1. Functional analyses showed that overexpression of Ches1 suppressed cell growth and arrested cells in the G2/M phase. Consistently, this gene has reduced expression in 52% of oral cancer tissues, which was significantly correlated with the areca nut chewing habit of patients (p =.04). Conclusion We identified 35 candidates and validated 6 genes that may be associated with areca nut-induced oral cancer. Loss of Ches1 may be attributed to areca nut extract-induced oral carcinogenesis.
KW - areca nut
KW - Ches1
KW - clinical association
KW - growth suppression
KW - oral cancer
UR - http://www.scopus.com/inward/record.url?scp=78751499344&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=78751499344&partnerID=8YFLogxK
U2 - 10.1002/hed.21442
DO - 10.1002/hed.21442
M3 - Article
C2 - 20848451
AN - SCOPUS:78751499344
VL - 33
SP - 257
EP - 266
JO - Head and Neck
JF - Head and Neck
SN - 1043-3074
IS - 2
ER -