Downregulation of caveolin-1 in a murine model of acute allergic airway disease

Chung Ming Chen, Meng Ying Wu, Hsiu Chu Chou, Yaw Dong Lang, Leng-Fang Wang

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: Airway remodeling refers to the structural changes in the airways of asthma. Caveolin-1 reduces cell growth and negatively regulates smooth muscle cell proliferation. The aim was to investigate lung caveolin-1 status in a murine model of acute allergic airway disease. Methods: Six- to eight-week-old female BALB/c mice were sensitized by intraperitoneal injections of phosphate-buffered saline or ovalbumin (OVA) and aluminium hydroxide on Days 0 and 14, challenged with aerosolized saline or OVA (1%) on Days 21-25, 28-32, and 35. The mice were killed 1 day after the last OVA/saline challenge. Serum OVA-specific immunoglobulin E (IgE) was measured by enzyme-linked immunosorbent assay. Peribronchial inflammation was quantified by morphometric analysis. Lung caveolin-1 and Type I collagen mRNA expression was determined by real-time reverse-transcription polymerase chain reaction. Total lung collagen was measured using Sircol Assay Kit. Results: Serum OVA-specific IgE levels were significantly elevated in OVA-challenged mice when compared with saline-challenged mice. Percentage of inflammatory cells in the bronchoalveolar lavage was significantly higher in the OVA-challenged animals. The animals' lungs that were sensitized and challenged with OVA contained large numbers of inflammatory cells concentrated near the airways and in the perivascular areas. The thickness of the bronchial epithelial layer and smooth muscle layer and the numbers of total inflammatory cells and eosinophils significantly increased in OVA-challenged mice. Caveolin-1 mRNA expression significantly decreased and Type I collagen mRNA expression significantly increased in the lung tissue of OVA-challenged mice. Conclusion: These results suggest that caveolin-1 seems to be involved in the pathogenesis of airway remodeling of acute allergic airway disease.

Original languageEnglish
Pages (from-to)5-10
Number of pages6
JournalPediatrics and Neonatology
Volume52
Issue number1
DOIs
Publication statusPublished - Feb 2011

Fingerprint

Caveolin 1
Ovalbumin
Down-Regulation
Lung
Airway Remodeling
Collagen Type I
Messenger RNA
Immunoglobulin E
Aluminum Hydroxide
Bronchoalveolar Lavage
Intraperitoneal Injections
Serum
Eosinophils
Reverse Transcription
Smooth Muscle Myocytes
Smooth Muscle
Collagen
Asthma
Cell Count
Enzyme-Linked Immunosorbent Assay

Keywords

  • acute allergic airway disease
  • airway remodeling
  • caveolin
  • collagen
  • mouse model

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health

Cite this

Downregulation of caveolin-1 in a murine model of acute allergic airway disease. / Chen, Chung Ming; Wu, Meng Ying; Chou, Hsiu Chu; Lang, Yaw Dong; Wang, Leng-Fang.

In: Pediatrics and Neonatology, Vol. 52, No. 1, 02.2011, p. 5-10.

Research output: Contribution to journalArticle

Chen, Chung Ming ; Wu, Meng Ying ; Chou, Hsiu Chu ; Lang, Yaw Dong ; Wang, Leng-Fang. / Downregulation of caveolin-1 in a murine model of acute allergic airway disease. In: Pediatrics and Neonatology. 2011 ; Vol. 52, No. 1. pp. 5-10.
@article{4edf20b7799a499db3ac387a7f2c50ec,
title = "Downregulation of caveolin-1 in a murine model of acute allergic airway disease",
abstract = "Background: Airway remodeling refers to the structural changes in the airways of asthma. Caveolin-1 reduces cell growth and negatively regulates smooth muscle cell proliferation. The aim was to investigate lung caveolin-1 status in a murine model of acute allergic airway disease. Methods: Six- to eight-week-old female BALB/c mice were sensitized by intraperitoneal injections of phosphate-buffered saline or ovalbumin (OVA) and aluminium hydroxide on Days 0 and 14, challenged with aerosolized saline or OVA (1{\%}) on Days 21-25, 28-32, and 35. The mice were killed 1 day after the last OVA/saline challenge. Serum OVA-specific immunoglobulin E (IgE) was measured by enzyme-linked immunosorbent assay. Peribronchial inflammation was quantified by morphometric analysis. Lung caveolin-1 and Type I collagen mRNA expression was determined by real-time reverse-transcription polymerase chain reaction. Total lung collagen was measured using Sircol Assay Kit. Results: Serum OVA-specific IgE levels were significantly elevated in OVA-challenged mice when compared with saline-challenged mice. Percentage of inflammatory cells in the bronchoalveolar lavage was significantly higher in the OVA-challenged animals. The animals' lungs that were sensitized and challenged with OVA contained large numbers of inflammatory cells concentrated near the airways and in the perivascular areas. The thickness of the bronchial epithelial layer and smooth muscle layer and the numbers of total inflammatory cells and eosinophils significantly increased in OVA-challenged mice. Caveolin-1 mRNA expression significantly decreased and Type I collagen mRNA expression significantly increased in the lung tissue of OVA-challenged mice. Conclusion: These results suggest that caveolin-1 seems to be involved in the pathogenesis of airway remodeling of acute allergic airway disease.",
keywords = "acute allergic airway disease, airway remodeling, caveolin, collagen, mouse model",
author = "Chen, {Chung Ming} and Wu, {Meng Ying} and Chou, {Hsiu Chu} and Lang, {Yaw Dong} and Leng-Fang Wang",
year = "2011",
month = "2",
doi = "10.1016/j.pedneo.2010.12.006",
language = "English",
volume = "52",
pages = "5--10",
journal = "Pediatrics and Neonatology",
issn = "1875-9572",
publisher = "臺灣兒科醫學會",
number = "1",

}

TY - JOUR

T1 - Downregulation of caveolin-1 in a murine model of acute allergic airway disease

AU - Chen, Chung Ming

AU - Wu, Meng Ying

AU - Chou, Hsiu Chu

AU - Lang, Yaw Dong

AU - Wang, Leng-Fang

PY - 2011/2

Y1 - 2011/2

N2 - Background: Airway remodeling refers to the structural changes in the airways of asthma. Caveolin-1 reduces cell growth and negatively regulates smooth muscle cell proliferation. The aim was to investigate lung caveolin-1 status in a murine model of acute allergic airway disease. Methods: Six- to eight-week-old female BALB/c mice were sensitized by intraperitoneal injections of phosphate-buffered saline or ovalbumin (OVA) and aluminium hydroxide on Days 0 and 14, challenged with aerosolized saline or OVA (1%) on Days 21-25, 28-32, and 35. The mice were killed 1 day after the last OVA/saline challenge. Serum OVA-specific immunoglobulin E (IgE) was measured by enzyme-linked immunosorbent assay. Peribronchial inflammation was quantified by morphometric analysis. Lung caveolin-1 and Type I collagen mRNA expression was determined by real-time reverse-transcription polymerase chain reaction. Total lung collagen was measured using Sircol Assay Kit. Results: Serum OVA-specific IgE levels were significantly elevated in OVA-challenged mice when compared with saline-challenged mice. Percentage of inflammatory cells in the bronchoalveolar lavage was significantly higher in the OVA-challenged animals. The animals' lungs that were sensitized and challenged with OVA contained large numbers of inflammatory cells concentrated near the airways and in the perivascular areas. The thickness of the bronchial epithelial layer and smooth muscle layer and the numbers of total inflammatory cells and eosinophils significantly increased in OVA-challenged mice. Caveolin-1 mRNA expression significantly decreased and Type I collagen mRNA expression significantly increased in the lung tissue of OVA-challenged mice. Conclusion: These results suggest that caveolin-1 seems to be involved in the pathogenesis of airway remodeling of acute allergic airway disease.

AB - Background: Airway remodeling refers to the structural changes in the airways of asthma. Caveolin-1 reduces cell growth and negatively regulates smooth muscle cell proliferation. The aim was to investigate lung caveolin-1 status in a murine model of acute allergic airway disease. Methods: Six- to eight-week-old female BALB/c mice were sensitized by intraperitoneal injections of phosphate-buffered saline or ovalbumin (OVA) and aluminium hydroxide on Days 0 and 14, challenged with aerosolized saline or OVA (1%) on Days 21-25, 28-32, and 35. The mice were killed 1 day after the last OVA/saline challenge. Serum OVA-specific immunoglobulin E (IgE) was measured by enzyme-linked immunosorbent assay. Peribronchial inflammation was quantified by morphometric analysis. Lung caveolin-1 and Type I collagen mRNA expression was determined by real-time reverse-transcription polymerase chain reaction. Total lung collagen was measured using Sircol Assay Kit. Results: Serum OVA-specific IgE levels were significantly elevated in OVA-challenged mice when compared with saline-challenged mice. Percentage of inflammatory cells in the bronchoalveolar lavage was significantly higher in the OVA-challenged animals. The animals' lungs that were sensitized and challenged with OVA contained large numbers of inflammatory cells concentrated near the airways and in the perivascular areas. The thickness of the bronchial epithelial layer and smooth muscle layer and the numbers of total inflammatory cells and eosinophils significantly increased in OVA-challenged mice. Caveolin-1 mRNA expression significantly decreased and Type I collagen mRNA expression significantly increased in the lung tissue of OVA-challenged mice. Conclusion: These results suggest that caveolin-1 seems to be involved in the pathogenesis of airway remodeling of acute allergic airway disease.

KW - acute allergic airway disease

KW - airway remodeling

KW - caveolin

KW - collagen

KW - mouse model

UR - http://www.scopus.com/inward/record.url?scp=79952442320&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952442320&partnerID=8YFLogxK

U2 - 10.1016/j.pedneo.2010.12.006

DO - 10.1016/j.pedneo.2010.12.006

M3 - Article

C2 - 21385650

AN - SCOPUS:79952442320

VL - 52

SP - 5

EP - 10

JO - Pediatrics and Neonatology

JF - Pediatrics and Neonatology

SN - 1875-9572

IS - 1

ER -