Downexpression of Matriptase-2 Correlates with Tumor Progression and Clinical Prognosis in Oral Squamous-Cell Carcinoma

Ming Fang Cheng, Li Han Lin, Mao Suan Huang, Herng Sheng Lee, Dar Der Ji, Chun Shu Lin, Kan Tai Hsia

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of this study was to investigate the relationship of matriptase-2 expression with the clinicopathologic characteristics, the histologic grade, and patient survival in oral squamous-cell carcinoma (OSCC). Immunohistochemical analysis of matriptase-2 expression was performed in 102 surgical specimens from patients with OSCC. The immunohistochemical results were further verified by quantitative real-time reverse transcription-polymerase chain reaction. The immunostaining intensity was scored on a scale ranging from 0 (absence of staining) to 3 (intense staining). The distribution score was determined by the percentage of stained cells on a scale ranging from 0 (<5%), 1 (5% to 25%), 2 (25% to 50%), 3 (50% to 75%), to 4 (75% to 100%). The immunoscore of matriptase-2 expression was the product of the above 2 scores and ranged from 0 to 12 for analysis. Faint matriptase-2 immunostaining was observed in the non-neoplastic oral mucosal epithelia. The matriptase-2 immunoscore was significantly higher in well-differentiated OSCCs than in poorly differentiated tumors (P=0.001). Moreover, a reduced matriptase-2 immunoscore was inversely correlated with the tumor size (P=0.017), a positive nodal stage (P=0.008), distant metastasis (P=0.032), and a late clinical stage (P=0.001). A lower immunoscore of matriptase-2 expression revealed a significant association with poor survival (P=0.003). Our results demonstrate that the inverse expression of matriptase-2 correlates with tumor progression and an advanced TNM stage, and has a poor prognosis in patients with OSCC. These findings suggest that the expression of matriptase-2 may be both a prognostic marker and a potential therapeutic target for this cancer.

Original languageEnglish
Pages (from-to)481-488
Number of pages8
JournalApplied Immunohistochemistry and Molecular Morphology
Volume25
Issue number7
DOIs
Publication statusPublished - 2017

Fingerprint

Squamous Cell Carcinoma
Neoplasms
Staining and Labeling
matriptase 2
Survival
Reverse Transcription
Epithelium
Neoplasm Metastasis
Polymerase Chain Reaction

Keywords

  • immunohistochemistry
  • matriptase-2
  • oral squamous-cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Histology
  • Medical Laboratory Technology

Cite this

Downexpression of Matriptase-2 Correlates with Tumor Progression and Clinical Prognosis in Oral Squamous-Cell Carcinoma. / Cheng, Ming Fang; Lin, Li Han; Huang, Mao Suan; Lee, Herng Sheng; Ji, Dar Der; Lin, Chun Shu; Hsia, Kan Tai.

In: Applied Immunohistochemistry and Molecular Morphology, Vol. 25, No. 7, 2017, p. 481-488.

Research output: Contribution to journalArticle

Cheng, Ming Fang ; Lin, Li Han ; Huang, Mao Suan ; Lee, Herng Sheng ; Ji, Dar Der ; Lin, Chun Shu ; Hsia, Kan Tai. / Downexpression of Matriptase-2 Correlates with Tumor Progression and Clinical Prognosis in Oral Squamous-Cell Carcinoma. In: Applied Immunohistochemistry and Molecular Morphology. 2017 ; Vol. 25, No. 7. pp. 481-488.
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AU - Ji, Dar Der

AU - Lin, Chun Shu

AU - Hsia, Kan Tai

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