Down-regulation of CD25 expression on the surface of activated tumor- infiltrating lymphocytes in human cervical carcinoma

Bor Ching Sheu, Rong Hwa Lin, Hong Nerng Ho, Su Cheng Huang

Research output: Contribution to journalArticle

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Abstract

To investigate the activation status of tumor-infiltrating lymphocytes (TILs) within the tumor milieu of human cervical carcinoma, we quantitatively measured and compared the activation markers on lymphocyte subpopulations which infiltrating normal and neoplastic cervix. A total of 20 patients with stage IA to IIA cervical cancer (cancer group) and 10 women with normal cervix (control group) were enrolled in this study. Mononuclear cells were isolated from tissue specimens by mechanical dispersal technique and three- color flow cytometry was utilized for the quantification of activation markers on lymphocyte subsets. Compared with control group, lymphocytes isolated from cancer tissue consisted of higher proportions of B cells (7.23% ± 4.49% vs. 3.67% ± 3.19%, P = 0.016) and T cells (72.33% ± 8.70% vs. 53.15% ± 17.36%, P = 0.004), but an inverted CD4:CD8 ratio (0.74 ± 0.27 vs, 1.14 ± 0.28, P = 0.002) and decreased NK cells (7.53% ± 4.33% vs. 16.00% ± 11.82%, P = 0.035). Low expression of CD25, but not CD69 and HLA-DR was observed on both CD4+CD3+ and CD8+CD3+ T cells derived from cervical cancer (P < 0.0001). Further dual activation marker analysis demonstrated that the expression of CD25 was dissociated from CD69 and HLA-DR on the same TILs in cancer tissue (P < 0.001). TILs in the tumor microenvironment can be functionally inhibited and lose the ability of clonal proliferation due to depressed expression of CD25.

Original languageEnglish
Pages (from-to)39-48
Number of pages10
JournalHuman Immunology
Volume56
Issue number1-2
DOIs
Publication statusPublished - Aug 1 1997
Externally publishedYes

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Immunologic Surveillance
T Lymphocyte Differentiation Antigens
CD3 Antigens
Tumor-Infiltrating Lymphocytes
Immunophenotyping
Interleukin-2 Receptors
Lymphocyte Subsets
Phytohemagglutinins
HLA-DR Antigens
Lymphocyte Activation
Uterine Cervical Neoplasms
Down-Regulation
Biomarkers
Carcinoma
T-Lymphocytes
Cervix Uteri
Neoplasms
Activation Analysis
CD4-CD8 Ratio
Control Groups

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Down-regulation of CD25 expression on the surface of activated tumor- infiltrating lymphocytes in human cervical carcinoma. / Sheu, Bor Ching; Lin, Rong Hwa; Ho, Hong Nerng; Huang, Su Cheng.

In: Human Immunology, Vol. 56, No. 1-2, 01.08.1997, p. 39-48.

Research output: Contribution to journalArticle

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