Down regulation of bcl-2 by p53 in nasopharyngeal carcinoma and lack of detection of its specific t(14;18) chromosomal translocation in fixed tissues

H. J. Harn, L. I. Ho, C. A. Liu, G. C. Liu, F. G. Lin, J. J. Lin, J. Y. Chang, W. H. Lee

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

High levels of bcl-2 protein have been found in a wide variety of human cancers. Since p53 gene inactivation occurs in over half of human cancers, it is possible that loss of p53-mediated repression of bcl-2 gene expression accounts, at least in part, for the frequent abnormalities in bcl-2 protein production seen in tumours. By using immunohistochemical methods, we have analysed thirty-three nasopharyngeal carcinomas for p53 and bcl-2 expression. We found an inverse correlation between the expression of these two proteins (P <0.001). Moreover, we utilized universal oligonucelotide primers of a region 5' to the bcl-2 MBR and at the 3' end of J(H) segments to initiate a DNA polymerase chain reaction that amplified these bcl-2-J(H) junctures. Of the twelve nasopharyngeal carcinomas expressing bcl-2, none showed a t(14;18) chromosome translocation. These findings may indicate potential mechanisms by which bcl-2 regulates apoptosis.

Original languageEnglish
Pages (from-to)317-323
Number of pages7
JournalHistopathology
Volume28
Issue number4
Publication statusPublished - 1996
Externally publishedYes

Keywords

  • Apoptosis
  • bcl-2 protein
  • Nasopharyngeal carcinoma
  • p53 protein

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Cell Biology

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