Dopaminergic D2 receptors activate PKA to inhibit spinal pelvic-urethra reflex in rats

Hsi Chin Wu, Chun Hsien Chiu, Kwong Chung Tung, Gin Den Chen, Hsien Yu Peng, Tzer Bin Lin

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

To clarify the role of descending dopaminergic innervation in reflexive urethral closure, the impacts of dopaminergic D2 receptor (DR2)-selective agonists and antagonists on repetitive stimulation-induced pelvic-to-urethra spinal reflex potentiation (SRP) were tested using in vivo rat preparations. Pelvic afferent nerve test stimulation (TS; 1 pulse/30 s for 30 min) evoked baseline reflex activity with single spikes in the external urethral sphincter electromyogram (EUSE), whereas, repetitive stimulation (RS; 1 pulse/s for 30 min) induced SRP. Intrathecal application of quinelorane dihydrochloride (Q110; 10, 30, and 100 nM, 10 μl, a selective DR2 agonist) dose dependently inhibited the RS-induced SRP. Pretreatment with L135 (100 nM, 10 μL it, a selective DR2 antagonist) antagonized the Q110-dependent inhibition (100 nM, 10 μl it). Intrathecal AMPA (10 μM, 10 μl, a selective glutamatergic AMPA receptor agonist), and NMDA (10 μM, 10 μl, a selective glutamatergic NMDA receptor agonist) reversed the Q110-dependent inhibition. Intrathecal forskolin (100 nM, 10 μl, a PKA activator) prevented the Q110-dependent inhibition that was reversed by CNQX (10 μM, 10 μl it, a selective glutamate AMPA receptor antagonist) and APV (10 μM, 10 μl it , a selective glutamate NMDA receptor antagonist). Our results suggest that DR2 activation, which inactivates intracellular PKA, may be involved in descending dopaminergic inhibition of NMDA/AMPA receptor-dependent SRP at the lumbosacral spinal cord, which is thought to be involved in reflexive urethral closure.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume299
Issue number3
DOIs
Publication statusPublished - Sep 2010
Externally publishedYes

Fingerprint

Urethra
Reflex
AMPA Receptors
N-Methyl-D-Aspartate Receptors
Excitatory Amino Acid Antagonists
quinelorane
6-Cyano-7-nitroquinoxaline-2,3-dione
alpha-Amino-3-hydroxy-5-methyl-4-isoxazolepropionic Acid
Electromyography
Colforsin
N-Methylaspartate
Spinal Cord
Inhibition (Psychology)

Keywords

  • cAMP
  • D2 receptor
  • L135
  • Q110
  • Rat
  • Urethra closure

ASJC Scopus subject areas

  • Physiology
  • Urology
  • Medicine(all)

Cite this

Dopaminergic D2 receptors activate PKA to inhibit spinal pelvic-urethra reflex in rats. / Wu, Hsi Chin; Chiu, Chun Hsien; Tung, Kwong Chung; Chen, Gin Den; Peng, Hsien Yu; Lin, Tzer Bin.

In: American Journal of Physiology - Renal Physiology, Vol. 299, No. 3, 09.2010.

Research output: Contribution to journalArticle

Wu, Hsi Chin ; Chiu, Chun Hsien ; Tung, Kwong Chung ; Chen, Gin Den ; Peng, Hsien Yu ; Lin, Tzer Bin. / Dopaminergic D2 receptors activate PKA to inhibit spinal pelvic-urethra reflex in rats. In: American Journal of Physiology - Renal Physiology. 2010 ; Vol. 299, No. 3.
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