Dopamine release impairment in striatum after different levels of cerebral cortical fluid percussion injury

Yuan Hao Chen, Eagle Yi Kung Huang, Tung Tai Kuo, Hsin I. Ma, Barry J. Hoffer, Pi Fen Tsui, Jing Jr Tsai, Yu Ching Chou, Yung-Hsiao Chiang

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

To investigate the role of dopamine release in cognitive impairment and motor learning deficits after brain injury, different levels of traumatic brain injury (TBI) were made in rats by using fluid percussion at two different atmospheres (2 Psi and 6 Psi). Tonic and phasic bursting dopamine release and behavior tests followed at several time points. We used in vitro fast-scan cyclic voltammetry to survey dopamine release in the striatum and analyzed the rats’ behavior using novel object recognition (NOR) and rotarod tests. Both tonic and bursting dopamine release were greatly depressed in the severely (6 Psi) injured group, which persisted up to 8 weeks later. However, in the 2 Psi-injured group, the suppression of bursting dopamine release occurred at 1~2 weeks after injury, but there were no significant differences after 4 weeks. Tonic dopamine release was also diminished significantly at 1~2 weeks after the injury; partial recovery could then be seen 4 weeks after injury. A significant deficiency in the fixed speed rotarod test and NOR test were noted in both 2 Psi and 6 Psi groups initially; however, the changes recovered in the 2 Psi group 2 weeks after injury while persisting in the 6 Psi group. In conclusion, striatal evoked dopamine release was affected by fluid percussion injury, with behavioral deficits showing differences as a function of injury severity. The severe fluid percussion injury (6 Psi) group showed more dopamine release defects, as well as cognitive and motor deficiencies. Recovery of dopamine release and improvement in behavioral impairment were better in the mild TBI group.

Original languageEnglish
Pages (from-to)2113-2128
Number of pages16
JournalCell Transplantation
Volume24
Issue number10
DOIs
Publication statusPublished - Oct 13 2015

Keywords

  • Dopamine
  • Dopamine reuptake
  • Fast-scan cyclic voltammetry (FSCV)
  • Fluid percussion injury
  • Novel object recognition (NOR) test

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

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    Chen, Y. H., Huang, E. Y. K., Kuo, T. T., Ma, H. I., Hoffer, B. J., Tsui, P. F., Tsai, J. J., Chou, Y. C., & Chiang, Y-H. (2015). Dopamine release impairment in striatum after different levels of cerebral cortical fluid percussion injury. Cell Transplantation, 24(10), 2113-2128. https://doi.org/10.3727/096368914X683584