DNA rearrangement mediated by inverted repeats

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

Inverted repeats of DNA are widespread in the genomes of eukaryotes and prokaryotes and can mediate genome rearrangement. We studied rearrangement mediated by plasmid-borne inverted repeats in Escherichia coli. We show that inverted repeats can mediate an efficient and recA-independent recombination event. Surprisingly, the product of this recombination is not that of simple inversion between the inverted repeats, but almost exclusively an unusual head-to-head dimer with complex DNA rearrangement. Moreover, this recombination is dramatically reduced by increasing the distance separating the repeats. These results can be readily explained by a model involving reciprocal switching of the leading and lagging strands of DNA replication within the inverted repeats, which leads to the formation of a Holliday junction. Reciprocal strand switching during DNA replication might be a common mechanism for genome rearrangement associated with inverted duplication.

Original languageEnglish
Pages (from-to)819-823
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number2
DOIs
Publication statusPublished - Jan 23 1996
Externally publishedYes

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Gene Rearrangement
Genetic Recombination
Genome
DNA Replication
Cruciform DNA
Eukaryota
Plasmids
Escherichia coli
DNA

ASJC Scopus subject areas

  • General
  • Genetics

Cite this

DNA rearrangement mediated by inverted repeats. / Bi, Xin; Liu, Leroy-Fong.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 93, No. 2, 23.01.1996, p. 819-823.

Research output: Contribution to journalArticle

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