DNA Fingerprintings of Five Chinese Herbal Medicines Revealed by RAPD Analysis

Kur-Ta Cheng, Ling-Ling Yang, Hsin-Sheng Tsay

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Abstract

The RAPD (random amplified polymorphic DNA) technique was employed to construct the fingerprints of five Chinese herbal medicines, Radix Bupleuri Chinensis, Radix Gentianae, Rhizoma Atractylodis Macrocephalae, Phizoma Coptidis, and Radix Angelicae Sinensis, all collected from stores in Taipei. In the present study, a decamer primer OPP-07:5'GTCCATGCCA3' was used in RAPD analysis to reveal the special fingerprints of the live herbal medicines mentioned above. The aim of the study is to provide a fast, easy tool to differentiate the Chinese herbs from their adulterants. The technique will contribute to the quality control of imported Chinese herbs.
Original languageEnglish
Pages (from-to)237-239
Number of pages3
Journal中醫藥雜誌
Volume7
Issue number4
Publication statusPublished - 1996

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Keywords

  • 逢機擴增多型性核酸反應
  • 中藥材
  • 指紋圖譜
  • RAPD
  • Chinese herb
  • Fingerprint

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