Diversity and enterotype in gut bacterial community of adults in Taiwan

Chao Liang, Han Chi Tseng, Hui Mei Chen, Wei Chi Wang, Chih Min Chiu, Jen Yun Chang, Kuan Yi Lu, Shun Long Weng, Tzu Hao Chang, Chao Hsiang Chang, Chen Tsung Weng, Hwei Ming Wang, Hsien Da Huang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Background: Gastrointestinal microbiota, particularly gut microbiota, is associated with human health. The biodiversity of gut microbiota is affected by ethnicities and environmental factors such as dietary habits or medicine intake, and three enterotypes of the human gut microbiome were announced in 2011. These enterotypes are not significantly correlated with gender, age, or body weight but are influenced by long-term dietary habits. However, to date, only two enterotypes (predominantly consisting of Bacteroides and Prevotella) have shown these characteristics in previous research; the third enterotype remains ambiguous. Understanding the enterotypes can improve the knowledge of the relationship between microbiota and human health. Results: We obtained 181 human fecal samples from adults in Taiwan. Microbiota compositions were analyzed using next-generation sequencing (NGS) technology, which is a culture-independent method of constructing microbial community profiles by sequencing 16S ribosomal DNA (rDNA). In these samples, 17,675,898 sequencing reads were sequenced, and on average, 215 operational taxonomic units (OTUs) were identified for each sample. In this study, the major bacteria in the enterotypes identified from the fecal samples were Bacteroides, Prevotella, and Enterobacteriaceae, and their correlation with dietary habits was confirmed. A microbial interaction network in the gut was observed on the basis of the amount of short-chain fatty acids, pH value of the intestine, and composition of the bacterial community (enterotypes). Finally, a decision tree was derived to provide a predictive model for the three enterotypes. The accuracies of this model in training and independent testing sets were 97.2 and 84.0%, respectively. Conclusions: We used NGS technology to characterize the microbiota and constructed a predictive model. The most significant finding was that Enterobacteriaceae, the predominant subtype, could be a new subtype of enterotypes in the Asian population.

Original languageEnglish
Article number932
JournalBMC Genomics
Volume18
DOIs
Publication statusPublished - Jan 25 2017

Fingerprint

Microbiota
Taiwan
Feeding Behavior
Prevotella
Bacteroides
Enterobacteriaceae
Microbial Interactions
Technology
Decision Trees
Volatile Fatty Acids
Biodiversity
Health
Ribosomal DNA
Intestines
Body Weight
Medicine
Bacteria
Gastrointestinal Microbiome
Research
Population

Keywords

  • 16S rDNA
  • Enterotype
  • Gut microbiome
  • Next-generation sequencing
  • Predictive model

ASJC Scopus subject areas

  • Biotechnology
  • Genetics

Cite this

Liang, C., Tseng, H. C., Chen, H. M., Wang, W. C., Chiu, C. M., Chang, J. Y., ... Huang, H. D. (2017). Diversity and enterotype in gut bacterial community of adults in Taiwan. BMC Genomics, 18, [932]. https://doi.org/10.1186/s12864-016-3261-6

Diversity and enterotype in gut bacterial community of adults in Taiwan. / Liang, Chao; Tseng, Han Chi; Chen, Hui Mei; Wang, Wei Chi; Chiu, Chih Min; Chang, Jen Yun; Lu, Kuan Yi; Weng, Shun Long; Chang, Tzu Hao; Chang, Chao Hsiang; Weng, Chen Tsung; Wang, Hwei Ming; Huang, Hsien Da.

In: BMC Genomics, Vol. 18, 932, 25.01.2017.

Research output: Contribution to journalArticle

Liang, C, Tseng, HC, Chen, HM, Wang, WC, Chiu, CM, Chang, JY, Lu, KY, Weng, SL, Chang, TH, Chang, CH, Weng, CT, Wang, HM & Huang, HD 2017, 'Diversity and enterotype in gut bacterial community of adults in Taiwan', BMC Genomics, vol. 18, 932. https://doi.org/10.1186/s12864-016-3261-6
Liang C, Tseng HC, Chen HM, Wang WC, Chiu CM, Chang JY et al. Diversity and enterotype in gut bacterial community of adults in Taiwan. BMC Genomics. 2017 Jan 25;18. 932. https://doi.org/10.1186/s12864-016-3261-6
Liang, Chao ; Tseng, Han Chi ; Chen, Hui Mei ; Wang, Wei Chi ; Chiu, Chih Min ; Chang, Jen Yun ; Lu, Kuan Yi ; Weng, Shun Long ; Chang, Tzu Hao ; Chang, Chao Hsiang ; Weng, Chen Tsung ; Wang, Hwei Ming ; Huang, Hsien Da. / Diversity and enterotype in gut bacterial community of adults in Taiwan. In: BMC Genomics. 2017 ; Vol. 18.
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AU - Lu, Kuan Yi

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