Diverse effects of type II collagen on osteogenic and adipogenic differentiation of mesenchymal stem cells

Li Hsuan Chiu, Tian-Shun Tsai, Huei Mei Huang, Sy Jye Leu, Charng Bin Yang, Yu-Hui Tsai

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18 Citations (Scopus)


Type II collagen is known to modulate chondrogenesis of mesenchymal stem cells (MSCs). In this study, MSCs from human bone marrow aspirates were used to study the modulating effects of type II collagen on MSC differentiation during the early stages of osteogenesis and adipogenesis. With osteogenic induction, MSCs cultured on the type II collagen-coated surface showed an enhanced calcium deposition level with increasing mRNA expressions of RUNX2, osteocalcin, and alkaline phosphatase. A synthetic integrin binding peptide, which specifically interacts with the I-domain of α 1β 12β 1 integrins significantly blocks the mineralization-enhancing effect of type II collagen. MSCs attached on the type II collagen-coated plates exhibited expanded cell morphology with increasing spreading area, and the pretreatment of cells with integrin α 1β 1 or α 2β 1-blocking antibody reduced the effect. The phosphorylation levels of FAK, ERK, and JNK significantly increased in the MSCs that attached on the type II collagen-coated plates. On the contrary, the mineralization-enhancing effect of type II collagen was diminished by JNK and MEK inhibitors. Furthermore, type II collagen blocked the adipogenic differentiation of MSCs, and this effect is rescued by JNK and MEK inhibitors. In conclusion, type II collagen facilitates osteogenesis and suppresses adipogenesis during early stage MSC differentiation. Such effects are integrin binding-mediated and conducted through FAK-JNK and/or FAK-ERK signaling cascades. These results inspire a novel strategy encompassing type II collagen in bone tissue engineering.

Original languageEnglish
Pages (from-to)2412-2420
Number of pages9
JournalJournal of Cellular Physiology
Issue number6
Publication statusPublished - Jun 2012


ASJC Scopus subject areas

  • Clinical Biochemistry
  • Cell Biology
  • Physiology

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