Diverse apoptotic pathways in enterovirus 71-infected cells

Shih Cheng Chang, Jing Yi Lin, Lily Yen Cheng Lo, Mei Ling Li, Shin Ru Shih

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Mechanisms related to the neuropathogenesis of enterovirus 71 infection remain unclear. This investigation conducts a comprehensive study of the apoptotic pathways in neural and non-neural cells following enterovirus 71 infection. Infections with enterovirus 71 not only induce classical cytopathic effects in SF268 (human glioblastoma), SK-N-MC (human neuroblastoma), RD, and Vero cells, but also induce classic signs of apoptosis in all cells, including DNA fragmentation and phosphatidylserine translocation. Apoptosis has also been caused by the efflux of cytochrome c from mitochondria, and subsequently by cleavage of caspase 9 in all cells. Activation of caspase 8 followed by cleavage of the proapoptotic protein Bid only occurs in non-neural cells. Results of this study demonstrate that a mitochondrial pathway of apoptosis mediated by activation and cleavage of caspase 9 is a main pathway in enterovirus 71-induced apoptosis, especially for enterovirus 71-infected neural cells.

Original languageEnglish
Pages (from-to)338-349
Number of pages12
JournalJournal of NeuroVirology
Volume10
Issue number6
DOIs
Publication statusPublished - Dec 2004
Externally publishedYes

Fingerprint

Enterovirus
Enterovirus Infections
Apoptosis
Caspase 9
BH3 Interacting Domain Death Agonist Protein
Neural Pathways
Vero Cells
Caspase 8
Phosphatidylserines
DNA Fragmentation
Glioblastoma
Cytochromes c
Neuroblastoma
Mitochondria

Keywords

  • Apoptosis
  • Caspase
  • Cytochrome c
  • Enterovirus 71
  • Mitochondria

ASJC Scopus subject areas

  • Virology
  • Clinical Neurology

Cite this

Chang, S. C., Lin, J. Y., Lo, L. Y. C., Li, M. L., & Shih, S. R. (2004). Diverse apoptotic pathways in enterovirus 71-infected cells. Journal of NeuroVirology, 10(6), 338-349. https://doi.org/10.1080/13550280490521032

Diverse apoptotic pathways in enterovirus 71-infected cells. / Chang, Shih Cheng; Lin, Jing Yi; Lo, Lily Yen Cheng; Li, Mei Ling; Shih, Shin Ru.

In: Journal of NeuroVirology, Vol. 10, No. 6, 12.2004, p. 338-349.

Research output: Contribution to journalArticle

Chang, SC, Lin, JY, Lo, LYC, Li, ML & Shih, SR 2004, 'Diverse apoptotic pathways in enterovirus 71-infected cells', Journal of NeuroVirology, vol. 10, no. 6, pp. 338-349. https://doi.org/10.1080/13550280490521032
Chang, Shih Cheng ; Lin, Jing Yi ; Lo, Lily Yen Cheng ; Li, Mei Ling ; Shih, Shin Ru. / Diverse apoptotic pathways in enterovirus 71-infected cells. In: Journal of NeuroVirology. 2004 ; Vol. 10, No. 6. pp. 338-349.
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