Divergence of genes encoding B chains of β-bungarotoxins

Yun Ching Cheng, Ku Chung Chen, Shu Kai Lin, Long Sen Chang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The structural organization of the genes encoding B2, B4, B5 and B6 chains of β-bungarotoxins are reported in this study. These genes shared virtually identical overall organization with three exons interrupted by two introns in similar positions. On the contrary, intron 1 of these genes had a similar size, a notable variation with the size of intron 2 was observed. It was found that two regions at the second intron of B1 and B2 chains were absent in that of B4, B5 and B6 chains. RT-PCR analyses indicated that Bungarus multicinctus venom gland, heart, liver and muscle expressed the RNA transcripts showing sequence similarity with the intronic segment being deleted in B4, B5 and B6 chain genes. This reflects that the ancestral gene of the intronic segment might insert in multiple loci of B. multicinctus genome. Comparative analyses of B chain genes showed that the protein-coding regions of the exons are more diverse than introns, except for in the signal peptide domain. These results suggest that intron insertions or deletions occur with the evolution of B chains, and that accelerated evolution may diversify the protein-coding sequence of B chain genes same as snake phospholipase A 2, neurotoxin and cardiotoxin genes.

Original languageEnglish
Pages (from-to)322-329
Number of pages8
JournalToxicon
Volume47
Issue number3
DOIs
Publication statusPublished - Mar 1 2006
Externally publishedYes

Fingerprint

Bungarotoxins
Gene encoding
Introns
Genes
Exons
Bungarus
Cardiotoxins
Snakes
Phospholipases A
Venoms
Neurotoxins
Protein Sorting Signals
Liver
Open Reading Frames
Muscle
Myocardium
Proteins
Genome
RNA
Polymerase Chain Reaction

Keywords

  • β-Bungarotoxin
  • B chain
  • Genomic structure
  • Intron insertion or deletion
  • Intronic RNA transcripts. Evolutionary divergence

ASJC Scopus subject areas

  • Toxicology

Cite this

Divergence of genes encoding B chains of β-bungarotoxins. / Cheng, Yun Ching; Chen, Ku Chung; Lin, Shu Kai; Chang, Long Sen.

In: Toxicon, Vol. 47, No. 3, 01.03.2006, p. 322-329.

Research output: Contribution to journalArticle

Cheng, Yun Ching ; Chen, Ku Chung ; Lin, Shu Kai ; Chang, Long Sen. / Divergence of genes encoding B chains of β-bungarotoxins. In: Toxicon. 2006 ; Vol. 47, No. 3. pp. 322-329.
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