Divaricoside Exerts Antitumor Effects, in Part, by Modulating Mcl-1 in Human Oral Squamous Cell Carcinoma Cells

Jing Ru Weng, Li Yuan Bai, Shih Jiuan Chiu, Chang Fang Chiu, Wei Yu Lin, Jing Lan Hu, Tzong Ming Shieh

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Cardiac glycosides (CGs), prescribed to treat congestive heart failure and arrhythmias, exert potent antitumor activity. In this study, divaricoside (DIV), a CG isolated from Strophanthus divaricatus was examined for its antitumor potency in oral squamous cell carcinoma (OSCC) cells. Cell growth was inhibited by DIV in a dose- and time-dependent manner in SCC2095 and OECM-1 OSCC cells using MTT assays. DIV induced S and G2/M phase arrest accompanied by downregulation of phosphorylated CDC25C, CDC25C, and CDC2 in SCC2095 cells. In addition, DIV induced apoptosis by activating caspase-3 and downregulating the expression of Mcl-1. Furthermore, overexpression of Mcl-1 partially reversed DIV-induced death in SCC2095 cells. Additionally, western blot and transmission electron microscopy analyses also indicated that DIV induced autophagy in SCC2095 cells. However, the combination of autophagy inhibitor did not affect DIV-mediated apoptosis in SCC2095 cells. Together, these findings suggest that translational potential of DIV to be developed as a therapeutic agent for OSCC treatment.

Original languageEnglish
Pages (from-to)151-159
Number of pages9
JournalComputational and Structural Biotechnology Journal
Volume17
DOIs
Publication statusPublished - Jan 1 2019

Keywords

  • Apoptosis
  • Autophagy
  • Cardiac glycoside
  • Divaricoside
  • Mcl-1
  • Oral squamous cell carcinoma

ASJC Scopus subject areas

  • Biotechnology
  • Biophysics
  • Structural Biology
  • Biochemistry
  • Genetics
  • Computer Science Applications

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