Distribution of Th1 and Th2 cell populations in human peripheral and decidual T cells from normal and anembryonic pregnancies

Hong Nerng Ho, Kuang Han Chao, Hsin Fu Chen, Shee Uan Chen, Ming Yih Wu, Yu Shih Yang

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Objective: To examine whether maternal immune responses during normal pregnancy are Th2 biased and whether there are specific changes when anembryonic pregnancy occurs. Design: Prospective study. Setting: Department of Obstetrics and Gynecology at a university hospital. Patient(s): We studied 32 pregnant women receiving elective abortions of normal pregnancies and 35 women with anembryonic pregnancies between 6 weeks and 10 weeks of gestational age. Intervention(s): Using the multilabeling capability of three-color flow cytometry, it is possible to measure intracellular cytokines and cell surface markers simultaneously to determine which cells are the cytokine-producing cells. Main Outcome Measure(s): We examined the extent and proportion of mononuclear cells expressing specific T-cell surface markers and cytokines, interferon γ, and interleukin 4 in the peripheral blood and deciduae. Secreted cytokines in the supernatants after 24-hour culture were also compared. Result(s): During the unstimulated status, the proportion of IL-4-secreting cells significantly exceeded that of IFN-γ-secreting cells in the peripheral blood and decidua in normal pregnancies and was significantly decreased when anembryonic pregnancies occurred. Consequently, the Th1/Th2 ratios were increased during anembryonic pregnancies. However, after 24-hour culture, only another Th2-type cytokine, IL-10, was markedly increased and exceeded IFN-γ secretion in cultures from both the peripheral blood and decidua in normal pregnancies. Conclusion(s): The decidual T lymphocytes are Th2 predominant. When anembryonic pregnancy occurs, this Th2 predominance disappears.

Original languageEnglish
Pages (from-to)797-803
Number of pages7
JournalFertility and Sterility
Volume76
Issue number4
DOIs
Publication statusPublished - Oct 16 2001
Externally publishedYes

Fingerprint

Decidua
Th2 Cells
Th1 Cells
Fetal Death
Flow Cytometry
Reference Values
Prospective Studies
Cytokines
T-Lymphocytes
Pregnancy
Population
Interleukin-4
Hospital Obstetrics and Gynecology Department
Gynecology
Interleukin-10
Gestational Age
Interferon-gamma
Pregnant Women
Color
Mothers

Keywords

  • Deciduae
  • Flow cytometry
  • Intracytoplasmic cytokines
  • Th1
  • Th2

ASJC Scopus subject areas

  • Obstetrics and Gynaecology

Cite this

Distribution of Th1 and Th2 cell populations in human peripheral and decidual T cells from normal and anembryonic pregnancies. / Ho, Hong Nerng; Chao, Kuang Han; Chen, Hsin Fu; Chen, Shee Uan; Wu, Ming Yih; Yang, Yu Shih.

In: Fertility and Sterility, Vol. 76, No. 4, 16.10.2001, p. 797-803.

Research output: Contribution to journalArticle

Ho, Hong Nerng ; Chao, Kuang Han ; Chen, Hsin Fu ; Chen, Shee Uan ; Wu, Ming Yih ; Yang, Yu Shih. / Distribution of Th1 and Th2 cell populations in human peripheral and decidual T cells from normal and anembryonic pregnancies. In: Fertility and Sterility. 2001 ; Vol. 76, No. 4. pp. 797-803.
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