Distribution of lysosome-associated membrane proteins-1 and-2, and cathepsin D in eosinophilic granular bodies: Possible relationship to cyst development in pilocytic astrocytomas

J. N. Tung, T. Y. Tsao, C. J. Tai, K. T. Yeh, Y. W. Cheng, M. C. Jiang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Pilocytic astrocytomas are usually cystic; cyst formation within these tumours may result in increased intracranial pressure, due to the effect of their mass, and contribute to cerebral damage. Eosinophilic granular bodies (EGBs) are produced abundantly in pilocytic astrocytomas but their role in disease progression remains unknown. Immunohistochemistry studies showed EGBs to exhibit pronounced reactivity to antibodies against lysosome-associated membrane proteins (LAMP)-1 and LAMP-2, and the lysosomal enzyme cathepsin D. Both LAMP-1 and LAMP-2 showed peripheral rim and granular staining patterns. The EGBs were scattered widely across cysts and, where EGBs aggregated in clusters, were usually close to areas of fluid in the cysts. Most EGBs had nuclei either attached or close by, indicating that the EGBs may be derived from anucleated astrocytes. The results suggest that EGBs, together with other factors, may play a role in the development of cysts in pilocytic astrocytomas.

Original languageEnglish
Pages (from-to)1354-1364
Number of pages11
JournalJournal of International Medical Research
Volume38
Issue number4
Publication statusPublished - 2010

Fingerprint

Lysosome-Associated Membrane Glycoproteins
Cathepsin D
Astrocytoma
Cysts
Cyst Fluid
Intracranial Pressure
Astrocytes
Disease Progression
Tumors
Immunohistochemistry
Staining and Labeling
Fluids
Antibodies
Enzymes
Neoplasms

Keywords

  • Cathepsin D
  • Cellular apoptosis susceptibility [CSE1L/CAS] protein
  • Cyst
  • Eosinophilic granular bodies
  • Lysosome-associated membrane proteins (LAMP)
  • Matrix metalloproteinase
  • Pilocytic astrocytomas

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry
  • Cell Biology
  • Biochemistry, medical

Cite this

Distribution of lysosome-associated membrane proteins-1 and-2, and cathepsin D in eosinophilic granular bodies : Possible relationship to cyst development in pilocytic astrocytomas. / Tung, J. N.; Tsao, T. Y.; Tai, C. J.; Yeh, K. T.; Cheng, Y. W.; Jiang, M. C.

In: Journal of International Medical Research, Vol. 38, No. 4, 2010, p. 1354-1364.

Research output: Contribution to journalArticle

@article{b88437c4e1fd4067850a68c0959ffed4,
title = "Distribution of lysosome-associated membrane proteins-1 and-2, and cathepsin D in eosinophilic granular bodies: Possible relationship to cyst development in pilocytic astrocytomas",
abstract = "Pilocytic astrocytomas are usually cystic; cyst formation within these tumours may result in increased intracranial pressure, due to the effect of their mass, and contribute to cerebral damage. Eosinophilic granular bodies (EGBs) are produced abundantly in pilocytic astrocytomas but their role in disease progression remains unknown. Immunohistochemistry studies showed EGBs to exhibit pronounced reactivity to antibodies against lysosome-associated membrane proteins (LAMP)-1 and LAMP-2, and the lysosomal enzyme cathepsin D. Both LAMP-1 and LAMP-2 showed peripheral rim and granular staining patterns. The EGBs were scattered widely across cysts and, where EGBs aggregated in clusters, were usually close to areas of fluid in the cysts. Most EGBs had nuclei either attached or close by, indicating that the EGBs may be derived from anucleated astrocytes. The results suggest that EGBs, together with other factors, may play a role in the development of cysts in pilocytic astrocytomas.",
keywords = "Cathepsin D, Cellular apoptosis susceptibility [CSE1L/CAS] protein, Cyst, Eosinophilic granular bodies, Lysosome-associated membrane proteins (LAMP), Matrix metalloproteinase, Pilocytic astrocytomas",
author = "Tung, {J. N.} and Tsao, {T. Y.} and Tai, {C. J.} and Yeh, {K. T.} and Cheng, {Y. W.} and Jiang, {M. C.}",
year = "2010",
language = "English",
volume = "38",
pages = "1354--1364",
journal = "Journal of International Medical Research",
issn = "0300-0605",
publisher = "Field House Publishing LLP",
number = "4",

}

TY - JOUR

T1 - Distribution of lysosome-associated membrane proteins-1 and-2, and cathepsin D in eosinophilic granular bodies

T2 - Possible relationship to cyst development in pilocytic astrocytomas

AU - Tung, J. N.

AU - Tsao, T. Y.

AU - Tai, C. J.

AU - Yeh, K. T.

AU - Cheng, Y. W.

AU - Jiang, M. C.

PY - 2010

Y1 - 2010

N2 - Pilocytic astrocytomas are usually cystic; cyst formation within these tumours may result in increased intracranial pressure, due to the effect of their mass, and contribute to cerebral damage. Eosinophilic granular bodies (EGBs) are produced abundantly in pilocytic astrocytomas but their role in disease progression remains unknown. Immunohistochemistry studies showed EGBs to exhibit pronounced reactivity to antibodies against lysosome-associated membrane proteins (LAMP)-1 and LAMP-2, and the lysosomal enzyme cathepsin D. Both LAMP-1 and LAMP-2 showed peripheral rim and granular staining patterns. The EGBs were scattered widely across cysts and, where EGBs aggregated in clusters, were usually close to areas of fluid in the cysts. Most EGBs had nuclei either attached or close by, indicating that the EGBs may be derived from anucleated astrocytes. The results suggest that EGBs, together with other factors, may play a role in the development of cysts in pilocytic astrocytomas.

AB - Pilocytic astrocytomas are usually cystic; cyst formation within these tumours may result in increased intracranial pressure, due to the effect of their mass, and contribute to cerebral damage. Eosinophilic granular bodies (EGBs) are produced abundantly in pilocytic astrocytomas but their role in disease progression remains unknown. Immunohistochemistry studies showed EGBs to exhibit pronounced reactivity to antibodies against lysosome-associated membrane proteins (LAMP)-1 and LAMP-2, and the lysosomal enzyme cathepsin D. Both LAMP-1 and LAMP-2 showed peripheral rim and granular staining patterns. The EGBs were scattered widely across cysts and, where EGBs aggregated in clusters, were usually close to areas of fluid in the cysts. Most EGBs had nuclei either attached or close by, indicating that the EGBs may be derived from anucleated astrocytes. The results suggest that EGBs, together with other factors, may play a role in the development of cysts in pilocytic astrocytomas.

KW - Cathepsin D

KW - Cellular apoptosis susceptibility [CSE1L/CAS] protein

KW - Cyst

KW - Eosinophilic granular bodies

KW - Lysosome-associated membrane proteins (LAMP)

KW - Matrix metalloproteinase

KW - Pilocytic astrocytomas

UR - http://www.scopus.com/inward/record.url?scp=77957744824&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77957744824&partnerID=8YFLogxK

M3 - Article

C2 - 20926008

AN - SCOPUS:77957744824

VL - 38

SP - 1354

EP - 1364

JO - Journal of International Medical Research

JF - Journal of International Medical Research

SN - 0300-0605

IS - 4

ER -