Distribution of ESBLs, AmpC β-lactamases and carbapenemases among Enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the Asia-Pacific region during 2008-14

Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART)

Shio Shin Jean, Po Ren Hsueh, Tony Korman, Justin Ellem, Narelle George, Geoffrey Coombs, Thomas Ling, Owen Tsang, V. Balaji, Hiroshige Mikamo, Shinya Kusachi, Tetsu Mizutani, Min Ja Kim, In Gyu Bae, Nurulhuda Binti Umur, Datin Ganeswrie Rajasekaram, Susan Taylor, Sally Roberts, Koen van der Werff, Dragana Drinkovic & 17 others Evelina Lagamayo, Myrna Mendoza, Thean Yen Tan, Prabha Krishnan, Ellie Wang, Po Liang Lu, Chun Eng Liu, Kenneth Yin Ching Chuang, Kwok Woon Yu, Yao Shen Chen, Min Chi Lu, Siripen Panthuwong, Pattarachai Kiratisin, Nguyen Tran My Phoung, Doan Mai Phuong, Nguyen Thi Quynh Ngoc, Tran Thi Thanh Nga

Research output: Contribution to journalArticle

33 Citations (Scopus)

Abstract

Objectives: To investigate the antimicrobial resistance and assess the molecular characteristics of β-lactamases (ESBLs, AmpC β-lactamases and carbapenemases) among Enterobacteriaceae isolates that caused intraabdominal infections (IAIs) in patients hospitalized in the Asia-Pacific region during 2008-14. Methods: Multiplex PCR was used to detect the specific types of β-lactamase in 2893 isolates with MICs of ertapenem.0.5 mg/L. In-hospital acquisition times for most isolates were also delineated. Results: Among 2728 (94.3%) isolates proven with β-lactamase production, the rates of non-susceptibility to imipenem were low (average=7.9%) among IAI Enterobacteriaceae isolates from all Asia-Pacific countries except Vietnam (17.7%) and the Philippines (10.2%). A stepwise and significant increase in annual rates of carbapenemase production among these isolates was noted. CTX-M-15 and CTX-M-14 were the dominant ESBL variants in most IAI Enterobacteriaceae species. The most abundant AmpC β-lactamase variants were blaCMY-2 among isolates of Escherichia coli and blaDHA-1 among isolates of Klebsiella pneumoniae. In addition, the IAI Enterobacteriaceae isolates harbouring a blaCMY-2 or blaDHA-1 allele were associated with high communityacquired rates (38.0% and 42.6%, respectively). AmpC ACT and MIR variants were mostly detected in Enterobacter species. The blaNDM-1,4,5,7-harbouring isolates of E. coli, K. pneumoniae and Enterobacter cloacae were most commonly identified among IAI isolates from Vietnam and the Philippines. Also of note, blaOXA-48- harbouring IAI Enterobacteriaceae isolates were detected exclusively in Vietnam. Conclusions: The high resistance burden in Vietnam and the Philippines warrants aggressive control policies to combat the worsening trend in antimicrobial resistance among Enterobacteriaceae species causing IAIs.

Original languageEnglish
Pages (from-to)166-171
Number of pages6
JournalJournal of Antimicrobial Chemotherapy
Volume72
Issue number1
DOIs
Publication statusPublished - 2017

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Intraabdominal Infections
Enterobacteriaceae
Urinary Tract Infections
Vietnam
Philippines
Klebsiella pneumoniae
Escherichia coli
Enterobacter cloacae
Enterobacter
Imipenem
Multiplex Polymerase Chain Reaction
Alleles

ASJC Scopus subject areas

  • Pharmacology
  • Pharmacology (medical)
  • Infectious Diseases

Cite this

Distribution of ESBLs, AmpC β-lactamases and carbapenemases among Enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the Asia-Pacific region during 2008-14 : Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART). / Jean, Shio Shin; Hsueh, Po Ren; Korman, Tony; Ellem, Justin; George, Narelle; Coombs, Geoffrey; Ling, Thomas; Tsang, Owen; Balaji, V.; Mikamo, Hiroshige; Kusachi, Shinya; Mizutani, Tetsu; Kim, Min Ja; Bae, In Gyu; Umur, Nurulhuda Binti; Rajasekaram, Datin Ganeswrie; Taylor, Susan; Roberts, Sally; van der Werff, Koen; Drinkovic, Dragana; Lagamayo, Evelina; Mendoza, Myrna; Tan, Thean Yen; Krishnan, Prabha; Wang, Ellie; Lu, Po Liang; Liu, Chun Eng; Chuang, Kenneth Yin Ching; Yu, Kwok Woon; Chen, Yao Shen; Lu, Min Chi; Panthuwong, Siripen; Kiratisin, Pattarachai; Phoung, Nguyen Tran My; Phuong, Doan Mai; Ngoc, Nguyen Thi Quynh; Nga, Tran Thi Thanh.

In: Journal of Antimicrobial Chemotherapy, Vol. 72, No. 1, 2017, p. 166-171.

Research output: Contribution to journalArticle

Jean, SS, Hsueh, PR, Korman, T, Ellem, J, George, N, Coombs, G, Ling, T, Tsang, O, Balaji, V, Mikamo, H, Kusachi, S, Mizutani, T, Kim, MJ, Bae, IG, Umur, NB, Rajasekaram, DG, Taylor, S, Roberts, S, van der Werff, K, Drinkovic, D, Lagamayo, E, Mendoza, M, Tan, TY, Krishnan, P, Wang, E, Lu, PL, Liu, CE, Chuang, KYC, Yu, KW, Chen, YS, Lu, MC, Panthuwong, S, Kiratisin, P, Phoung, NTM, Phuong, DM, Ngoc, NTQ & Nga, TTT 2017, 'Distribution of ESBLs, AmpC β-lactamases and carbapenemases among Enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the Asia-Pacific region during 2008-14: Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART)', Journal of Antimicrobial Chemotherapy, vol. 72, no. 1, pp. 166-171. https://doi.org/10.1093/jac/dkw398
Jean, Shio Shin ; Hsueh, Po Ren ; Korman, Tony ; Ellem, Justin ; George, Narelle ; Coombs, Geoffrey ; Ling, Thomas ; Tsang, Owen ; Balaji, V. ; Mikamo, Hiroshige ; Kusachi, Shinya ; Mizutani, Tetsu ; Kim, Min Ja ; Bae, In Gyu ; Umur, Nurulhuda Binti ; Rajasekaram, Datin Ganeswrie ; Taylor, Susan ; Roberts, Sally ; van der Werff, Koen ; Drinkovic, Dragana ; Lagamayo, Evelina ; Mendoza, Myrna ; Tan, Thean Yen ; Krishnan, Prabha ; Wang, Ellie ; Lu, Po Liang ; Liu, Chun Eng ; Chuang, Kenneth Yin Ching ; Yu, Kwok Woon ; Chen, Yao Shen ; Lu, Min Chi ; Panthuwong, Siripen ; Kiratisin, Pattarachai ; Phoung, Nguyen Tran My ; Phuong, Doan Mai ; Ngoc, Nguyen Thi Quynh ; Nga, Tran Thi Thanh. / Distribution of ESBLs, AmpC β-lactamases and carbapenemases among Enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the Asia-Pacific region during 2008-14 : Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART). In: Journal of Antimicrobial Chemotherapy. 2017 ; Vol. 72, No. 1. pp. 166-171.
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title = "Distribution of ESBLs, AmpC β-lactamases and carbapenemases among Enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the Asia-Pacific region during 2008-14: Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART)",
abstract = "Objectives: To investigate the antimicrobial resistance and assess the molecular characteristics of β-lactamases (ESBLs, AmpC β-lactamases and carbapenemases) among Enterobacteriaceae isolates that caused intraabdominal infections (IAIs) in patients hospitalized in the Asia-Pacific region during 2008-14. Methods: Multiplex PCR was used to detect the specific types of β-lactamase in 2893 isolates with MICs of ertapenem.0.5 mg/L. In-hospital acquisition times for most isolates were also delineated. Results: Among 2728 (94.3{\%}) isolates proven with β-lactamase production, the rates of non-susceptibility to imipenem were low (average=7.9{\%}) among IAI Enterobacteriaceae isolates from all Asia-Pacific countries except Vietnam (17.7{\%}) and the Philippines (10.2{\%}). A stepwise and significant increase in annual rates of carbapenemase production among these isolates was noted. CTX-M-15 and CTX-M-14 were the dominant ESBL variants in most IAI Enterobacteriaceae species. The most abundant AmpC β-lactamase variants were blaCMY-2 among isolates of Escherichia coli and blaDHA-1 among isolates of Klebsiella pneumoniae. In addition, the IAI Enterobacteriaceae isolates harbouring a blaCMY-2 or blaDHA-1 allele were associated with high communityacquired rates (38.0{\%} and 42.6{\%}, respectively). AmpC ACT and MIR variants were mostly detected in Enterobacter species. The blaNDM-1,4,5,7-harbouring isolates of E. coli, K. pneumoniae and Enterobacter cloacae were most commonly identified among IAI isolates from Vietnam and the Philippines. Also of note, blaOXA-48- harbouring IAI Enterobacteriaceae isolates were detected exclusively in Vietnam. Conclusions: The high resistance burden in Vietnam and the Philippines warrants aggressive control policies to combat the worsening trend in antimicrobial resistance among Enterobacteriaceae species causing IAIs.",
author = "Jean, {Shio Shin} and Hsueh, {Po Ren} and Tony Korman and Justin Ellem and Narelle George and Geoffrey Coombs and Thomas Ling and Owen Tsang and V. Balaji and Hiroshige Mikamo and Shinya Kusachi and Tetsu Mizutani and Kim, {Min Ja} and Bae, {In Gyu} and Umur, {Nurulhuda Binti} and Rajasekaram, {Datin Ganeswrie} and Susan Taylor and Sally Roberts and {van der Werff}, Koen and Dragana Drinkovic and Evelina Lagamayo and Myrna Mendoza and Tan, {Thean Yen} and Prabha Krishnan and Ellie Wang and Lu, {Po Liang} and Liu, {Chun Eng} and Chuang, {Kenneth Yin Ching} and Yu, {Kwok Woon} and Chen, {Yao Shen} and Lu, {Min Chi} and Siripen Panthuwong and Pattarachai Kiratisin and Phoung, {Nguyen Tran My} and Phuong, {Doan Mai} and Ngoc, {Nguyen Thi Quynh} and Nga, {Tran Thi Thanh}",
year = "2017",
doi = "10.1093/jac/dkw398",
language = "English",
volume = "72",
pages = "166--171",
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TY - JOUR

T1 - Distribution of ESBLs, AmpC β-lactamases and carbapenemases among Enterobacteriaceae isolates causing intra-abdominal and urinary tract infections in the Asia-Pacific region during 2008-14

T2 - Results from the Study for Monitoring Antimicrobial Resistance Trends (SMART)

AU - Jean, Shio Shin

AU - Hsueh, Po Ren

AU - Korman, Tony

AU - Ellem, Justin

AU - George, Narelle

AU - Coombs, Geoffrey

AU - Ling, Thomas

AU - Tsang, Owen

AU - Balaji, V.

AU - Mikamo, Hiroshige

AU - Kusachi, Shinya

AU - Mizutani, Tetsu

AU - Kim, Min Ja

AU - Bae, In Gyu

AU - Umur, Nurulhuda Binti

AU - Rajasekaram, Datin Ganeswrie

AU - Taylor, Susan

AU - Roberts, Sally

AU - van der Werff, Koen

AU - Drinkovic, Dragana

AU - Lagamayo, Evelina

AU - Mendoza, Myrna

AU - Tan, Thean Yen

AU - Krishnan, Prabha

AU - Wang, Ellie

AU - Lu, Po Liang

AU - Liu, Chun Eng

AU - Chuang, Kenneth Yin Ching

AU - Yu, Kwok Woon

AU - Chen, Yao Shen

AU - Lu, Min Chi

AU - Panthuwong, Siripen

AU - Kiratisin, Pattarachai

AU - Phoung, Nguyen Tran My

AU - Phuong, Doan Mai

AU - Ngoc, Nguyen Thi Quynh

AU - Nga, Tran Thi Thanh

PY - 2017

Y1 - 2017

N2 - Objectives: To investigate the antimicrobial resistance and assess the molecular characteristics of β-lactamases (ESBLs, AmpC β-lactamases and carbapenemases) among Enterobacteriaceae isolates that caused intraabdominal infections (IAIs) in patients hospitalized in the Asia-Pacific region during 2008-14. Methods: Multiplex PCR was used to detect the specific types of β-lactamase in 2893 isolates with MICs of ertapenem.0.5 mg/L. In-hospital acquisition times for most isolates were also delineated. Results: Among 2728 (94.3%) isolates proven with β-lactamase production, the rates of non-susceptibility to imipenem were low (average=7.9%) among IAI Enterobacteriaceae isolates from all Asia-Pacific countries except Vietnam (17.7%) and the Philippines (10.2%). A stepwise and significant increase in annual rates of carbapenemase production among these isolates was noted. CTX-M-15 and CTX-M-14 were the dominant ESBL variants in most IAI Enterobacteriaceae species. The most abundant AmpC β-lactamase variants were blaCMY-2 among isolates of Escherichia coli and blaDHA-1 among isolates of Klebsiella pneumoniae. In addition, the IAI Enterobacteriaceae isolates harbouring a blaCMY-2 or blaDHA-1 allele were associated with high communityacquired rates (38.0% and 42.6%, respectively). AmpC ACT and MIR variants were mostly detected in Enterobacter species. The blaNDM-1,4,5,7-harbouring isolates of E. coli, K. pneumoniae and Enterobacter cloacae were most commonly identified among IAI isolates from Vietnam and the Philippines. Also of note, blaOXA-48- harbouring IAI Enterobacteriaceae isolates were detected exclusively in Vietnam. Conclusions: The high resistance burden in Vietnam and the Philippines warrants aggressive control policies to combat the worsening trend in antimicrobial resistance among Enterobacteriaceae species causing IAIs.

AB - Objectives: To investigate the antimicrobial resistance and assess the molecular characteristics of β-lactamases (ESBLs, AmpC β-lactamases and carbapenemases) among Enterobacteriaceae isolates that caused intraabdominal infections (IAIs) in patients hospitalized in the Asia-Pacific region during 2008-14. Methods: Multiplex PCR was used to detect the specific types of β-lactamase in 2893 isolates with MICs of ertapenem.0.5 mg/L. In-hospital acquisition times for most isolates were also delineated. Results: Among 2728 (94.3%) isolates proven with β-lactamase production, the rates of non-susceptibility to imipenem were low (average=7.9%) among IAI Enterobacteriaceae isolates from all Asia-Pacific countries except Vietnam (17.7%) and the Philippines (10.2%). A stepwise and significant increase in annual rates of carbapenemase production among these isolates was noted. CTX-M-15 and CTX-M-14 were the dominant ESBL variants in most IAI Enterobacteriaceae species. The most abundant AmpC β-lactamase variants were blaCMY-2 among isolates of Escherichia coli and blaDHA-1 among isolates of Klebsiella pneumoniae. In addition, the IAI Enterobacteriaceae isolates harbouring a blaCMY-2 or blaDHA-1 allele were associated with high communityacquired rates (38.0% and 42.6%, respectively). AmpC ACT and MIR variants were mostly detected in Enterobacter species. The blaNDM-1,4,5,7-harbouring isolates of E. coli, K. pneumoniae and Enterobacter cloacae were most commonly identified among IAI isolates from Vietnam and the Philippines. Also of note, blaOXA-48- harbouring IAI Enterobacteriaceae isolates were detected exclusively in Vietnam. Conclusions: The high resistance burden in Vietnam and the Philippines warrants aggressive control policies to combat the worsening trend in antimicrobial resistance among Enterobacteriaceae species causing IAIs.

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