Distribution and expression of CD200 in the rat respiratory system under normal and endotoxin-induced pathological conditions

Jiang Shieh Ya Fen, Hsiung-Fei Chien, Chiu-Yun Chang, Tsui Shan Wei, Mei Miao Chiu, Hui Min Chen, Ching-Hsiang Wu

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

In vivo and in vitro studies have clearly demonstrated that signaling mediated by the interaction of CD200 and its cognate receptor, CD200R, results in an attenuation of inflammatory or autoimmune responses through multiple mechanisms. The present results have shown a differential expression of CD200 in the respiratory tract of intact rats. Along the respiratory passage, CD200 was specifically distributed at the bronchiolar epithelia with intense CD200 immunoreactivity localized at the apical surface of some ciliated epithelial cells; only a limited expression was detected on the Clara cells extending into the alveolar duct. In the alveolar septum, double immunofluorescence showed intense CD200 immunolabeling on the capillary endothelia. A moderate CD200 labeling was observed on the alveolar type II epithelial cells. It was, however, absent in the alveolar type I epithelial cells and the alveolar macrophages. Immunoelectron microscopic study has revealed a specific distribution of CD200 on the luminal front of the thin portion of alveolar endothelia. During endotoxemia, the injured lungs showed a dose- and time-dependent decline of CD200 expression accompanied by a vigorous infiltration of immune cells, some of them expressing ionized calcium binding adapter protein 1 or CD200. Ultrastructural examination further showed that the marked reduction of CD200 expression was mainly attributable to the loss of alveolar endothelial CD200. It is therefore suggested that CD200 expressed by different lung cells may play diverse roles in immune homeostasis of normal lung, in particular, the molecules on alveolar endothelia that may control regular recruitment of immune cells via CD200-CD200R interaction. Additionally, it may contribute to intense infiltration of immune cells following the loss or inefficiency of CD200 under pathological conditions.

Original languageEnglish
Pages (from-to)407-416
Number of pages10
JournalJournal of Anatomy
Volume216
Issue number3
DOIs
Publication statusPublished - Mar 2010

Fingerprint

endotoxins
respiratory system
Endotoxins
Respiratory System
infiltration
endothelium
rats
homeostasis
Alveolar Epithelial Cells
epithelial cells
lungs
calcium
Lung
Endothelium
cells
protein
calcium-binding proteins
endotoxemia
autoimmunity
Calcium-Binding Proteins

Keywords

  • Endotoxin
  • Immunoglobulin superfamily
  • Lung epithelia
  • Pulmonary endothelia

ASJC Scopus subject areas

  • Anatomy
  • Histology
  • Developmental Biology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Cell Biology

Cite this

Distribution and expression of CD200 in the rat respiratory system under normal and endotoxin-induced pathological conditions. / Ya Fen, Jiang Shieh; Chien, Hsiung-Fei; Chang, Chiu-Yun; Wei, Tsui Shan; Chiu, Mei Miao; Chen, Hui Min; Wu, Ching-Hsiang.

In: Journal of Anatomy, Vol. 216, No. 3, 03.2010, p. 407-416.

Research output: Contribution to journalArticle

Ya Fen, Jiang Shieh ; Chien, Hsiung-Fei ; Chang, Chiu-Yun ; Wei, Tsui Shan ; Chiu, Mei Miao ; Chen, Hui Min ; Wu, Ching-Hsiang. / Distribution and expression of CD200 in the rat respiratory system under normal and endotoxin-induced pathological conditions. In: Journal of Anatomy. 2010 ; Vol. 216, No. 3. pp. 407-416.
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