Distinct subpopulations of head and neck cancer cells with different levels of intracellular reactive oxygen species exhibit diverse stemness, proliferation, and chemosensitivity

Ching Wen Chang, Yu Syuan Chen, Shiu Huey Chou, Chia Li Han, Yu Ju Chen, Cheng Chieh Yang, Chih Yang Huang, Jeng Fan Lo

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

Head and neck squamous cell carcinoma (HNSCC) is driven by cancer-initiating cells (CIC), but their maintenance mechanisms are obscure. For hematopoietic stem cells, low levels of intracellular reactive oxygen species (ROSLow) is known to help sustain stemness properties. In this report, we evaluated the hypothesis that ROSLow character conferred CIC properties in HNSCC. Sphere cultures define CIC in HNSCC cell populations (HN-CIC). We found that ROSLow cells in HN-CIC defined in this manner were more numerous than in parental HNSCC cells. Further, ROSLow cells frequently coexpressed CIC surface markers such as memGrp78 and Glut3. Exploiting flow cytometry to sort cells on the basis of their ROS level, we found that isolated ROSLow cells displayed relatively more CIC properties, including quiescence, chemoresistance, in vitro malignant properties, and tumorigenicity. Pharmacological depletion of ROS modulators in cisplatin-treated HN-CIC reduced CIC properties, enhancing cell differentiation and enhancing cisplatin-induced cell death. Overall, our work defined cell subpopulations in HNSCC on the basis of differential intracellular ROS levels, which associated with stemness and chemoresistance properties. On the basis of our findings, we suggest that strategies to promote intracellular ROS levels may heighten the efficacy of conventional chemotherapy used for HNSCC treatment.

Original languageEnglish
Pages (from-to)6291-6305
Number of pages15
JournalCancer Research
Volume74
Issue number21
DOIs
Publication statusPublished - Nov 1 2014
Externally publishedYes

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Head and Neck Neoplasms
Reactive Oxygen Species
Neoplasms
Cisplatin
Hematopoietic Stem Cells
Carcinoma, squamous cell of head and neck
Cell Differentiation

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Medicine(all)

Cite this

Distinct subpopulations of head and neck cancer cells with different levels of intracellular reactive oxygen species exhibit diverse stemness, proliferation, and chemosensitivity. / Chang, Ching Wen; Chen, Yu Syuan; Chou, Shiu Huey; Han, Chia Li; Chen, Yu Ju; Yang, Cheng Chieh; Huang, Chih Yang; Lo, Jeng Fan.

In: Cancer Research, Vol. 74, No. 21, 01.11.2014, p. 6291-6305.

Research output: Contribution to journalArticle

Chang, Ching Wen ; Chen, Yu Syuan ; Chou, Shiu Huey ; Han, Chia Li ; Chen, Yu Ju ; Yang, Cheng Chieh ; Huang, Chih Yang ; Lo, Jeng Fan. / Distinct subpopulations of head and neck cancer cells with different levels of intracellular reactive oxygen species exhibit diverse stemness, proliferation, and chemosensitivity. In: Cancer Research. 2014 ; Vol. 74, No. 21. pp. 6291-6305.
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