Distinct regulation of cyclooxygenase-2 by interleukin-1β in normal and endometriotic stromal cells

Meng Hsing Wu, Chu An Wang, Chen Chung Lin, Lei Chin Chen, Wen Chang Chang, Shaw Jenq Tsai

Research output: Contribution to journalArticle

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Abstract

Aberrant production of cyclooxygenase-2 (COX-2) plays pivotal roles in many pathological processes including tumorigenesis and endometriosis, although the underlying mechanism remains obscure. Herein we report evidence to demonstrate that COX-2 is distinctly regulated by IL-1β in normal and endomeiriotic stroma. Ectopic endometriotic stromal cell is at least 100 times more sensitive to IL-1β treatment, compared with its eutopic counterpart. Induction of COX-2 expression in normal endometrial stroma by IL-1β is primary due to enhancement of COX-2 mRNA stability. In contrast, IL-1β not only increases COX-2 mRNA stability but also up-regulates COX-2 promoter activity in eciopic endometriotic stroma. Induction of COX-2 promoter activity by IL-1β is mediated via MAPK-dependent phosphorylation of cAMP-responding element binding protein. Promoter activity and EMSAs demonstrate that a cAMP response element site located at -571/-564 of COX-2 promoter is critical for IL-1β-induced COX-2 gene expression. Our results indicate that elevation of COX-2 expression in endometriotic tissues may result from increased sensitivity to proinflammatory cytokines such as IL-1β, which is consistently present in the peritoneal fluid of endometriosis patients. Distinct regulation of COX-2 gene by IL-1β may play a critical role in pathopliysiological processes such as cancer formation and endometriosis.

Original languageEnglish
Pages (from-to)286-295
Number of pages10
JournalJournal of Clinical Endocrinology and Metabolism
Volume90
Issue number1
DOIs
Publication statusPublished - Jan 2005
Externally publishedYes

Fingerprint

Cyclooxygenase 2
Stromal Cells
Interleukin-1
Endometriosis
RNA Stability
Messenger RNA
Phosphorylation
Ascitic Fluid
Response Elements
Pathologic Processes
Gene expression
Carrier Proteins
Carcinogenesis
Up-Regulation
Genes
Tissue
Cytokines
Gene Expression
Fluids

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

Distinct regulation of cyclooxygenase-2 by interleukin-1β in normal and endometriotic stromal cells. / Wu, Meng Hsing; Wang, Chu An; Lin, Chen Chung; Chen, Lei Chin; Chang, Wen Chang; Tsai, Shaw Jenq.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 90, No. 1, 01.2005, p. 286-295.

Research output: Contribution to journalArticle

Wu, Meng Hsing ; Wang, Chu An ; Lin, Chen Chung ; Chen, Lei Chin ; Chang, Wen Chang ; Tsai, Shaw Jenq. / Distinct regulation of cyclooxygenase-2 by interleukin-1β in normal and endometriotic stromal cells. In: Journal of Clinical Endocrinology and Metabolism. 2005 ; Vol. 90, No. 1. pp. 286-295.
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