Distinct metabolic changes in human lung cancer cells with differential radiation sensitivities

Cheng Ying Shen, Ching Hua Kuo, San Yuan Wang, Wei Qing Yang, Chun Ting Kuo, Yufeng J. Tseng, Mong Hsun Tsai

Research output: Contribution to journalArticle

Abstract

Background: Radiotherapy is one of the most important modalities for cancer treatment. However, the radio-resistance of malignant tumor cells is the major cause of radiation treatment failure. Although several biochemical pathways are disturbed in response to radiation, the metabolite profiles related to radio-resistance have not been well investigated in malignant non-small cell lung carcinomas (NSCLCs). Malignant lung cancer cells, CL1-5, were more resistant to radiation than were the parental CL1-0 cells. Therefore, the two cell lines provide a suitable cell model to investigate changes in metabolite profiles related to radiation exposure. Methods: Two human lung cancer cell lines, CL1-5 and CL1-0, with differential radiation sensitivities, were irradiated at a dosage of 10 Gy and harvested at 1, 4, and 24 hr after radiation treatment. Proton nuclear magnetic resonance (1H-NMR) was used to analyze the metabolic profiles of the CL1-0 and CL1-5 cell lines. The metabolite profiles of the cell extracts were subjected to principal component analysis (PCA). Both PCA and specific metabolite data were used to examine metabolic differences between the two cell lines. Results: The colony formation assay results demonstrate that CL1-5 cells are more sensitive than CL1-0 cells to irradiation. The PCA score plots for NMR spectra of CL1-0 and CL1-5 cells identified metabolites such as glutathione, creatine phosphate, glutamate, o-phosphocholine, pyroglutamate, taurine, and trimethylamine n-oxide (TMAO) as key molecules with high correlation in response to different irradiation tolerance. The metabolite profiles of the cell lines were inherent different, but following 24-hr irradiation, common metabolite alterations were induced in both cell lines. Moreover, CL1-0 cells showed a larger difference in the elevation of glutathione levels than CL1-5 cells. Conclusions: Our study demonstrates a clear difference in metabolite profiles between irradiation-resistant and irradiation-sensitive cells. These metabolite profile changes could be used to elucidate the possible mechanism of radio-resistance.

Original languageEnglish
Pages (from-to)738-747
Number of pages10
JournalTranslational Cancer Research
Volume5
Issue number6
DOIs
Publication statusPublished - Jan 1 2016
Externally publishedYes

Fingerprint

Radiation Tolerance
Lung Neoplasms
Cell Line
Principal Component Analysis
Radio
Radiation
Glutathione
Pyrrolidonecarboxylic Acid
Phosphorylcholine
Phosphocreatine
Metabolome
Taurine
Cell Extracts
Treatment Failure
Non-Small Cell Lung Carcinoma
Protons
Glutamic Acid
Neoplasms
Magnetic Resonance Spectroscopy
Radiotherapy

Keywords

  • CL1-0
  • CL1-5
  • Lung cancer
  • Metabolites
  • Radiation

ASJC Scopus subject areas

  • Oncology
  • Radiology Nuclear Medicine and imaging
  • Cancer Research

Cite this

Distinct metabolic changes in human lung cancer cells with differential radiation sensitivities. / Shen, Cheng Ying; Kuo, Ching Hua; Wang, San Yuan; Yang, Wei Qing; Kuo, Chun Ting; Tseng, Yufeng J.; Tsai, Mong Hsun.

In: Translational Cancer Research, Vol. 5, No. 6, 01.01.2016, p. 738-747.

Research output: Contribution to journalArticle

Shen, Cheng Ying ; Kuo, Ching Hua ; Wang, San Yuan ; Yang, Wei Qing ; Kuo, Chun Ting ; Tseng, Yufeng J. ; Tsai, Mong Hsun. / Distinct metabolic changes in human lung cancer cells with differential radiation sensitivities. In: Translational Cancer Research. 2016 ; Vol. 5, No. 6. pp. 738-747.
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abstract = "Background: Radiotherapy is one of the most important modalities for cancer treatment. However, the radio-resistance of malignant tumor cells is the major cause of radiation treatment failure. Although several biochemical pathways are disturbed in response to radiation, the metabolite profiles related to radio-resistance have not been well investigated in malignant non-small cell lung carcinomas (NSCLCs). Malignant lung cancer cells, CL1-5, were more resistant to radiation than were the parental CL1-0 cells. Therefore, the two cell lines provide a suitable cell model to investigate changes in metabolite profiles related to radiation exposure. Methods: Two human lung cancer cell lines, CL1-5 and CL1-0, with differential radiation sensitivities, were irradiated at a dosage of 10 Gy and harvested at 1, 4, and 24 hr after radiation treatment. Proton nuclear magnetic resonance (1H-NMR) was used to analyze the metabolic profiles of the CL1-0 and CL1-5 cell lines. The metabolite profiles of the cell extracts were subjected to principal component analysis (PCA). Both PCA and specific metabolite data were used to examine metabolic differences between the two cell lines. Results: The colony formation assay results demonstrate that CL1-5 cells are more sensitive than CL1-0 cells to irradiation. The PCA score plots for NMR spectra of CL1-0 and CL1-5 cells identified metabolites such as glutathione, creatine phosphate, glutamate, o-phosphocholine, pyroglutamate, taurine, and trimethylamine n-oxide (TMAO) as key molecules with high correlation in response to different irradiation tolerance. The metabolite profiles of the cell lines were inherent different, but following 24-hr irradiation, common metabolite alterations were induced in both cell lines. Moreover, CL1-0 cells showed a larger difference in the elevation of glutathione levels than CL1-5 cells. Conclusions: Our study demonstrates a clear difference in metabolite profiles between irradiation-resistant and irradiation-sensitive cells. These metabolite profile changes could be used to elucidate the possible mechanism of radio-resistance.",
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AU - Kuo, Ching Hua

AU - Wang, San Yuan

AU - Yang, Wei Qing

AU - Kuo, Chun Ting

AU - Tseng, Yufeng J.

AU - Tsai, Mong Hsun

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N2 - Background: Radiotherapy is one of the most important modalities for cancer treatment. However, the radio-resistance of malignant tumor cells is the major cause of radiation treatment failure. Although several biochemical pathways are disturbed in response to radiation, the metabolite profiles related to radio-resistance have not been well investigated in malignant non-small cell lung carcinomas (NSCLCs). Malignant lung cancer cells, CL1-5, were more resistant to radiation than were the parental CL1-0 cells. Therefore, the two cell lines provide a suitable cell model to investigate changes in metabolite profiles related to radiation exposure. Methods: Two human lung cancer cell lines, CL1-5 and CL1-0, with differential radiation sensitivities, were irradiated at a dosage of 10 Gy and harvested at 1, 4, and 24 hr after radiation treatment. Proton nuclear magnetic resonance (1H-NMR) was used to analyze the metabolic profiles of the CL1-0 and CL1-5 cell lines. The metabolite profiles of the cell extracts were subjected to principal component analysis (PCA). Both PCA and specific metabolite data were used to examine metabolic differences between the two cell lines. Results: The colony formation assay results demonstrate that CL1-5 cells are more sensitive than CL1-0 cells to irradiation. The PCA score plots for NMR spectra of CL1-0 and CL1-5 cells identified metabolites such as glutathione, creatine phosphate, glutamate, o-phosphocholine, pyroglutamate, taurine, and trimethylamine n-oxide (TMAO) as key molecules with high correlation in response to different irradiation tolerance. The metabolite profiles of the cell lines were inherent different, but following 24-hr irradiation, common metabolite alterations were induced in both cell lines. Moreover, CL1-0 cells showed a larger difference in the elevation of glutathione levels than CL1-5 cells. Conclusions: Our study demonstrates a clear difference in metabolite profiles between irradiation-resistant and irradiation-sensitive cells. These metabolite profile changes could be used to elucidate the possible mechanism of radio-resistance.

AB - Background: Radiotherapy is one of the most important modalities for cancer treatment. However, the radio-resistance of malignant tumor cells is the major cause of radiation treatment failure. Although several biochemical pathways are disturbed in response to radiation, the metabolite profiles related to radio-resistance have not been well investigated in malignant non-small cell lung carcinomas (NSCLCs). Malignant lung cancer cells, CL1-5, were more resistant to radiation than were the parental CL1-0 cells. Therefore, the two cell lines provide a suitable cell model to investigate changes in metabolite profiles related to radiation exposure. Methods: Two human lung cancer cell lines, CL1-5 and CL1-0, with differential radiation sensitivities, were irradiated at a dosage of 10 Gy and harvested at 1, 4, and 24 hr after radiation treatment. Proton nuclear magnetic resonance (1H-NMR) was used to analyze the metabolic profiles of the CL1-0 and CL1-5 cell lines. The metabolite profiles of the cell extracts were subjected to principal component analysis (PCA). Both PCA and specific metabolite data were used to examine metabolic differences between the two cell lines. Results: The colony formation assay results demonstrate that CL1-5 cells are more sensitive than CL1-0 cells to irradiation. The PCA score plots for NMR spectra of CL1-0 and CL1-5 cells identified metabolites such as glutathione, creatine phosphate, glutamate, o-phosphocholine, pyroglutamate, taurine, and trimethylamine n-oxide (TMAO) as key molecules with high correlation in response to different irradiation tolerance. The metabolite profiles of the cell lines were inherent different, but following 24-hr irradiation, common metabolite alterations were induced in both cell lines. Moreover, CL1-0 cells showed a larger difference in the elevation of glutathione levels than CL1-5 cells. Conclusions: Our study demonstrates a clear difference in metabolite profiles between irradiation-resistant and irradiation-sensitive cells. These metabolite profile changes could be used to elucidate the possible mechanism of radio-resistance.

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