Dissemination of imipenem-resistant Acinetobacter baumannii with new plasmid-borne blaOXA-72 in Taiwan

Shu Chen Kuo, Su Pen Yang, Yi Tzu Lee, Han Chuan Chuang, Chien Pei Chen, Chi Ling Chang, Te Li Chen, Po Liang Lu, Po Ren Hsueh, Chang Phone Fung

Research output: Contribution to journalArticle

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Abstract

Background: The systemic surveillance of imipenem-resistant Acinetobacter baumannii (IRAB) from multicenters in Taiwan revealed the emergence of isolates with blaOXA-72. This study described their genetic makeup, mechanism of spread, and contribution to carbapenem resistance.Methods: Two hundred and ninety-one non-repetitive isolates of A. baumannii were collected from 10 teaching hospitals from different geographical regions in Taiwan from June 2007 to September 2007. Minimal inhibitory concentrations (MICs) were determined by agar dilution. Clonality was determined by pulsed-field gel electrophoresis. Plasmid was extracted and digested by restriction enzymes, and subsequently analyzed by electrophoresis and Southern blot for blaOXA-72. The flanking regions of blaOXA-72 were determined by inverse PCR. The contribution of blaOXA-72 to imipenem MIC was determined by transforming plasmids carrying blaOXA-72 into imipenem-susceptible A. baumannii.Results: Among 142 IRAB in Taiwan, 27 harbored blaOXA-72; 22 originated from Southern Taiwan, 5 from Central Taiwan, and none from Northern Taiwan. There were two major clones. The blaOXA-72 was identified in the plasmids of all isolates. Two genetic structures flanking plasmid-borne blaOXA-72 were identified and shared identical sequences in certain regions; the one described in previous literature was present in only one isolate, and the new one was present in the remaining isolates. Introduction of blaOXA-72 resulted in an increase of imipenem MIC in the transformants. The overexpression of blaOXA-72 mRNA in response to imipenem further supported the contribution of blaOXA-72.. Conclusions: In conclusion, isolates with new plasmid-borne blaOXA-72 were found to be disseminated successfully in Southern Taiwan. The spread of the resistance gene depended on clonal spread and dissemination of a new plasmid. BlaOXA-72 in these isolates directly led to their imipenem-resistance.

Original languageEnglish
Article number319
JournalBMC Infectious Diseases
Volume13
Issue number1
DOIs
Publication statusPublished - Jul 13 2013

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Acinetobacter baumannii
Imipenem
Taiwan
Plasmids
Carbapenems
Genetic Structures
Pulsed Field Gel Electrophoresis
Southern Blotting
Teaching Hospitals
Agar
Electrophoresis
Clone Cells
Polymerase Chain Reaction
Messenger RNA
Enzymes
Genes

Keywords

  • Acinetobacter baumannii
  • BlaOXA-72
  • Carbapenemase
  • Imipenem-resistant

ASJC Scopus subject areas

  • Infectious Diseases

Cite this

Dissemination of imipenem-resistant Acinetobacter baumannii with new plasmid-borne blaOXA-72 in Taiwan. / Kuo, Shu Chen; Yang, Su Pen; Lee, Yi Tzu; Chuang, Han Chuan; Chen, Chien Pei; Chang, Chi Ling; Chen, Te Li; Lu, Po Liang; Hsueh, Po Ren; Fung, Chang Phone.

In: BMC Infectious Diseases, Vol. 13, No. 1, 319, 13.07.2013.

Research output: Contribution to journalArticle

Kuo, SC, Yang, SP, Lee, YT, Chuang, HC, Chen, CP, Chang, CL, Chen, TL, Lu, PL, Hsueh, PR & Fung, CP 2013, 'Dissemination of imipenem-resistant Acinetobacter baumannii with new plasmid-borne blaOXA-72 in Taiwan', BMC Infectious Diseases, vol. 13, no. 1, 319. https://doi.org/10.1186/1471-2334-13-319
Kuo, Shu Chen ; Yang, Su Pen ; Lee, Yi Tzu ; Chuang, Han Chuan ; Chen, Chien Pei ; Chang, Chi Ling ; Chen, Te Li ; Lu, Po Liang ; Hsueh, Po Ren ; Fung, Chang Phone. / Dissemination of imipenem-resistant Acinetobacter baumannii with new plasmid-borne blaOXA-72 in Taiwan. In: BMC Infectious Diseases. 2013 ; Vol. 13, No. 1.
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AU - Lee, Yi Tzu

AU - Chuang, Han Chuan

AU - Chen, Chien Pei

AU - Chang, Chi Ling

AU - Chen, Te Li

AU - Lu, Po Liang

AU - Hsueh, Po Ren

AU - Fung, Chang Phone

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N2 - Background: The systemic surveillance of imipenem-resistant Acinetobacter baumannii (IRAB) from multicenters in Taiwan revealed the emergence of isolates with blaOXA-72. This study described their genetic makeup, mechanism of spread, and contribution to carbapenem resistance.Methods: Two hundred and ninety-one non-repetitive isolates of A. baumannii were collected from 10 teaching hospitals from different geographical regions in Taiwan from June 2007 to September 2007. Minimal inhibitory concentrations (MICs) were determined by agar dilution. Clonality was determined by pulsed-field gel electrophoresis. Plasmid was extracted and digested by restriction enzymes, and subsequently analyzed by electrophoresis and Southern blot for blaOXA-72. The flanking regions of blaOXA-72 were determined by inverse PCR. The contribution of blaOXA-72 to imipenem MIC was determined by transforming plasmids carrying blaOXA-72 into imipenem-susceptible A. baumannii.Results: Among 142 IRAB in Taiwan, 27 harbored blaOXA-72; 22 originated from Southern Taiwan, 5 from Central Taiwan, and none from Northern Taiwan. There were two major clones. The blaOXA-72 was identified in the plasmids of all isolates. Two genetic structures flanking plasmid-borne blaOXA-72 were identified and shared identical sequences in certain regions; the one described in previous literature was present in only one isolate, and the new one was present in the remaining isolates. Introduction of blaOXA-72 resulted in an increase of imipenem MIC in the transformants. The overexpression of blaOXA-72 mRNA in response to imipenem further supported the contribution of blaOXA-72.. Conclusions: In conclusion, isolates with new plasmid-borne blaOXA-72 were found to be disseminated successfully in Southern Taiwan. The spread of the resistance gene depended on clonal spread and dissemination of a new plasmid. BlaOXA-72 in these isolates directly led to their imipenem-resistance.

AB - Background: The systemic surveillance of imipenem-resistant Acinetobacter baumannii (IRAB) from multicenters in Taiwan revealed the emergence of isolates with blaOXA-72. This study described their genetic makeup, mechanism of spread, and contribution to carbapenem resistance.Methods: Two hundred and ninety-one non-repetitive isolates of A. baumannii were collected from 10 teaching hospitals from different geographical regions in Taiwan from June 2007 to September 2007. Minimal inhibitory concentrations (MICs) were determined by agar dilution. Clonality was determined by pulsed-field gel electrophoresis. Plasmid was extracted and digested by restriction enzymes, and subsequently analyzed by electrophoresis and Southern blot for blaOXA-72. The flanking regions of blaOXA-72 were determined by inverse PCR. The contribution of blaOXA-72 to imipenem MIC was determined by transforming plasmids carrying blaOXA-72 into imipenem-susceptible A. baumannii.Results: Among 142 IRAB in Taiwan, 27 harbored blaOXA-72; 22 originated from Southern Taiwan, 5 from Central Taiwan, and none from Northern Taiwan. There were two major clones. The blaOXA-72 was identified in the plasmids of all isolates. Two genetic structures flanking plasmid-borne blaOXA-72 were identified and shared identical sequences in certain regions; the one described in previous literature was present in only one isolate, and the new one was present in the remaining isolates. Introduction of blaOXA-72 resulted in an increase of imipenem MIC in the transformants. The overexpression of blaOXA-72 mRNA in response to imipenem further supported the contribution of blaOXA-72.. Conclusions: In conclusion, isolates with new plasmid-borne blaOXA-72 were found to be disseminated successfully in Southern Taiwan. The spread of the resistance gene depended on clonal spread and dissemination of a new plasmid. BlaOXA-72 in these isolates directly led to their imipenem-resistance.

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KW - Carbapenemase

KW - Imipenem-resistant

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