Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood

Yu Jen Yu, Tzu-Bi Shih, Ching Ping Tseng, Tzu Bou Hsieh, Don Ching Lee, Shiaw Min Hwang

Research output: Contribution to journalArticle

160 Citations (Scopus)

Abstract

Bone marrow and umbilical cord blood are reported to be the main sources of mesenchymal stem cells (MSCs), which have been proposed for many clinical applications. This study evaluated and quantitated the differentiation potential of bone marrow-derived MSCs (bmMSCs) and cord blood-derived MSCs (cbMSCs) by in vitro induction. Results indicated that cbMSCs had a significantly stronger osteogenic potential but lower capacity for adipogenic differentiation than bmMSCs. Leptin, an important regulator of mesenchymal differentiation, has a significantly stronger effect of promoting osteogenesis and inhibiting adipogenesis in bmMSCs than in cbMSCs. Moreover, Cbfa1 mRNA expression in bmMSCs and cbMSCs was affected to different degrees by leptin during osteogenesis. In contrast, leptin reduced PPARγ2 mRNA expression to the same level during adipogenesis in both types of MSCs. These results demonstrate the disparate capacities of MSCs from bone marrow and cord blood and suggest that they be used differently in experimental and therapeutic studies. In addition, the disparate differentiation tendencies of MSCs from different sources should be considered in further applications.

Original languageEnglish
Pages (from-to)679-685
Number of pages7
JournalStem Cells
Volume24
Issue number3
DOIs
Publication statusPublished - Mar 2006

Fingerprint

Mesoderm
Mesenchymal Stromal Cells
Fetal Blood
Bone Marrow
Leptin
Adipogenesis
Osteogenesis
Messenger RNA

Keywords

  • Differentiation
  • Leptin
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Cell Biology

Cite this

Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood. / Yu, Yu Jen; Shih, Tzu-Bi; Tseng, Ching Ping; Hsieh, Tzu Bou; Lee, Don Ching; Hwang, Shiaw Min.

In: Stem Cells, Vol. 24, No. 3, 03.2006, p. 679-685.

Research output: Contribution to journalArticle

Yu, Yu Jen ; Shih, Tzu-Bi ; Tseng, Ching Ping ; Hsieh, Tzu Bou ; Lee, Don Ching ; Hwang, Shiaw Min. / Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood. In: Stem Cells. 2006 ; Vol. 24, No. 3. pp. 679-685.
@article{c9d3be35bfbe4e479e8162ee509acb24,
title = "Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood",
abstract = "Bone marrow and umbilical cord blood are reported to be the main sources of mesenchymal stem cells (MSCs), which have been proposed for many clinical applications. This study evaluated and quantitated the differentiation potential of bone marrow-derived MSCs (bmMSCs) and cord blood-derived MSCs (cbMSCs) by in vitro induction. Results indicated that cbMSCs had a significantly stronger osteogenic potential but lower capacity for adipogenic differentiation than bmMSCs. Leptin, an important regulator of mesenchymal differentiation, has a significantly stronger effect of promoting osteogenesis and inhibiting adipogenesis in bmMSCs than in cbMSCs. Moreover, Cbfa1 mRNA expression in bmMSCs and cbMSCs was affected to different degrees by leptin during osteogenesis. In contrast, leptin reduced PPARγ2 mRNA expression to the same level during adipogenesis in both types of MSCs. These results demonstrate the disparate capacities of MSCs from bone marrow and cord blood and suggest that they be used differently in experimental and therapeutic studies. In addition, the disparate differentiation tendencies of MSCs from different sources should be considered in further applications.",
keywords = "Differentiation, Leptin, Mesenchymal stem cells",
author = "Yu, {Yu Jen} and Tzu-Bi Shih and Tseng, {Ching Ping} and Hsieh, {Tzu Bou} and Lee, {Don Ching} and Hwang, {Shiaw Min}",
year = "2006",
month = "3",
doi = "10.1634/stemcells.2004-0308",
language = "English",
volume = "24",
pages = "679--685",
journal = "Stem Cells",
issn = "1066-5099",
publisher = "AlphaMed Press",
number = "3",

}

TY - JOUR

T1 - Disparate mesenchyme-lineage tendencies in mesenchymal stem cells from human bone marrow and umbilical cord blood

AU - Yu, Yu Jen

AU - Shih, Tzu-Bi

AU - Tseng, Ching Ping

AU - Hsieh, Tzu Bou

AU - Lee, Don Ching

AU - Hwang, Shiaw Min

PY - 2006/3

Y1 - 2006/3

N2 - Bone marrow and umbilical cord blood are reported to be the main sources of mesenchymal stem cells (MSCs), which have been proposed for many clinical applications. This study evaluated and quantitated the differentiation potential of bone marrow-derived MSCs (bmMSCs) and cord blood-derived MSCs (cbMSCs) by in vitro induction. Results indicated that cbMSCs had a significantly stronger osteogenic potential but lower capacity for adipogenic differentiation than bmMSCs. Leptin, an important regulator of mesenchymal differentiation, has a significantly stronger effect of promoting osteogenesis and inhibiting adipogenesis in bmMSCs than in cbMSCs. Moreover, Cbfa1 mRNA expression in bmMSCs and cbMSCs was affected to different degrees by leptin during osteogenesis. In contrast, leptin reduced PPARγ2 mRNA expression to the same level during adipogenesis in both types of MSCs. These results demonstrate the disparate capacities of MSCs from bone marrow and cord blood and suggest that they be used differently in experimental and therapeutic studies. In addition, the disparate differentiation tendencies of MSCs from different sources should be considered in further applications.

AB - Bone marrow and umbilical cord blood are reported to be the main sources of mesenchymal stem cells (MSCs), which have been proposed for many clinical applications. This study evaluated and quantitated the differentiation potential of bone marrow-derived MSCs (bmMSCs) and cord blood-derived MSCs (cbMSCs) by in vitro induction. Results indicated that cbMSCs had a significantly stronger osteogenic potential but lower capacity for adipogenic differentiation than bmMSCs. Leptin, an important regulator of mesenchymal differentiation, has a significantly stronger effect of promoting osteogenesis and inhibiting adipogenesis in bmMSCs than in cbMSCs. Moreover, Cbfa1 mRNA expression in bmMSCs and cbMSCs was affected to different degrees by leptin during osteogenesis. In contrast, leptin reduced PPARγ2 mRNA expression to the same level during adipogenesis in both types of MSCs. These results demonstrate the disparate capacities of MSCs from bone marrow and cord blood and suggest that they be used differently in experimental and therapeutic studies. In addition, the disparate differentiation tendencies of MSCs from different sources should be considered in further applications.

KW - Differentiation

KW - Leptin

KW - Mesenchymal stem cells

UR - http://www.scopus.com/inward/record.url?scp=33744727071&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33744727071&partnerID=8YFLogxK

U2 - 10.1634/stemcells.2004-0308

DO - 10.1634/stemcells.2004-0308

M3 - Article

VL - 24

SP - 679

EP - 685

JO - Stem Cells

JF - Stem Cells

SN - 1066-5099

IS - 3

ER -