Disintegrin modulates rat glomerular mesangial cell behavior

T. J. Tsai, J. R. Sheu, Y. M. Chen, C. J. Yen, C. F. Chen, T. F. Huang

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Disintegrins are a group of molecules containing Arg-Gly-Asp (RGD) sequence which can interfere with cell-matrix interaction. Rat mesangial cells are known to express an RGD-sensitive integrin receptor. We investigate the effect of a potent disintegrin, triflavin, on rat mesangial cell adhesion and proliferation. While synthetic RGD-containing peptide (GRGDS) inhibits mesangial cell adhesion to fibronectin, type I and type III collagen, triflavin has a similar but much more potent effect. Triflavin, when added to the medium, inhibits serum-stimulated rat mesangial cell proliferation in both growing and growth-arrested cells. In serum-free medium, platelet-derived growth factor promoted mesangial cell growth is also inhibited by triflavin. When coated as a substratum, both GRGDS and triflavin inhibit mesangial cell growth. The inhibition of proliferation by exogenously added triflavin and GRGDS is, at least in part, due to their antiadhesive effect. We conclude that triflavin can modulate both adhesion and proliferation of rat mesangial cells.

Original languageEnglish
Pages (from-to)83-90
Number of pages8
JournalNephron
Volume70
Issue number1
Publication statusPublished - 1995
Externally publishedYes

Fingerprint

Disintegrins
Mesangial Cells
glycyl-arginyl-glycyl-aspartyl-serine
Cell Adhesion
Growth
Cell Proliferation
Collagen Type III
Platelet-Derived Growth Factor
Serum-Free Culture Media
Collagen Type I
triflavin
Fibronectins
Integrins
Cell Communication
Serum

Keywords

  • Disintegrin
  • Mesangial cell

ASJC Scopus subject areas

  • Nephrology

Cite this

Tsai, T. J., Sheu, J. R., Chen, Y. M., Yen, C. J., Chen, C. F., & Huang, T. F. (1995). Disintegrin modulates rat glomerular mesangial cell behavior. Nephron, 70(1), 83-90.

Disintegrin modulates rat glomerular mesangial cell behavior. / Tsai, T. J.; Sheu, J. R.; Chen, Y. M.; Yen, C. J.; Chen, C. F.; Huang, T. F.

In: Nephron, Vol. 70, No. 1, 1995, p. 83-90.

Research output: Contribution to journalArticle

Tsai, TJ, Sheu, JR, Chen, YM, Yen, CJ, Chen, CF & Huang, TF 1995, 'Disintegrin modulates rat glomerular mesangial cell behavior', Nephron, vol. 70, no. 1, pp. 83-90.
Tsai TJ, Sheu JR, Chen YM, Yen CJ, Chen CF, Huang TF. Disintegrin modulates rat glomerular mesangial cell behavior. Nephron. 1995;70(1):83-90.
Tsai, T. J. ; Sheu, J. R. ; Chen, Y. M. ; Yen, C. J. ; Chen, C. F. ; Huang, T. F. / Disintegrin modulates rat glomerular mesangial cell behavior. In: Nephron. 1995 ; Vol. 70, No. 1. pp. 83-90.
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