Disease tropism of c-erbB: Effects of carboxyl-terminal tyrosine and internal mutations on tissue-specific transformation

R. J. Pelley, N. J. Maihle, C. Boerkoel, H. K. Shu, T. H. Carter, C. Moscovici, H. J. Kung

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Avian leukosis virus induces erythroleukemia in chickens by proviral insertional mutation of the proto-oncogene c-erbB. The product of the insertionally activated c-erbB locus lacks the extracellular ligand-binding domain and is strictly leukemogenic. It has previously been demonstrated that the disease spectrum associated with aberrant c-erbB expression can be expanded by structural perturbation of the cytoplasmic domain of this protein. In this report, we use mutagenesis and retroviral vectors to identify specific mutations in the carboxyl-terminal domain of the insertionally activated c-erbB product that are sufficient to activate the sarcomagenic potential of this protein. Interestingly, a point mutation in the kinase domain appears to be sufficient for sarcomagenic activation. However, removal of the terminal tyrosine residue of the c-erbB product, implicated in modulating kinase activity, does not lead to a fully transforming phenotype. These studies suggest that there are multiple ways to activate the fibroblast-transforming potential of the insertionally activated c-erbB product. The conformation of this protein may play a more significant role in oncogenic activation than the phosphorylation status of the putative carboxyl-terminal autophosphorylation site.

Original languageEnglish
Pages (from-to)7164-7168
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume86
Issue number18
DOIs
Publication statusPublished - Jan 1 1989
Externally publishedYes

ASJC Scopus subject areas

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