Direct visualization of microcirculation impairment after acute subdural hemorrhage in a novel animal model

Huan Chih Wang, Jui Chang Tsai, Jing Er Lee, Sheng Jean Huang, Abel Po-Hao Huang, Wei Chou Lin, Sung Tsang Hsieh, Kuo Chuan Wang

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

OBJECTIVE Direct brain compression and secondary injury due to increased intracranial pressure are believed to be the pathognomic causes of a grave outcome in acute subdural hemorrhage (aSDH). However, ischemic damage from aSDH has received limited attention. The authors hypothesized that cerebral microcirculation is altered after aSDH. Direct visualization of microcirculation was conducted in a novel rat model. METHODS A craniectomy was performed on each of the 18 experimental adult Wistar rats, followed by superfusion of autologous arterial blood onto the cortical surface. Changes in microcirculation were recorded by capillary videoscopy. Blood flow and the partial pressure of oxygen in the brain tissue (PbtO2) were measured at various depths from the cortex. The brain was then sectioned for pathological examination. The effects of aspirin pretreatment were also examined. RESULTS Instantaneous vasospasm of small cortical arteries after aSDH was observed; thrombosis also developed 120 minutes after aSDH. Reductions in blood flow and PbtO2were found at depths of 2-4 mm. Blood-brain barrier disruption and thrombi formation were confirmed using immunohistochemical staining, while aspirin pretreatment reduced thrombosis and the impairment of microcirculation. CONCLUSIONS Microcirculation impairment was demonstrated in this aSDH model. Aspirin pretreatment prevented the diffuse thrombosis of cortical and subcortical vessels after aSDH.

Original languageEnglish
Pages (from-to)997-1007
Number of pages11
JournalJournal of Neurosurgery
Volume129
Issue number4
DOIs
Publication statusPublished - Oct 2018

Keywords

  • acute subdural hemorrhage
  • aspirin
  • microcirculation
  • rat model
  • trauma

ASJC Scopus subject areas

  • Surgery
  • Clinical Neurology

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    Wang, H. C., Tsai, J. C., Lee, J. E., Huang, S. J., Po-Hao Huang, A., Lin, W. C., Hsieh, S. T., & Wang, K. C. (2018). Direct visualization of microcirculation impairment after acute subdural hemorrhage in a novel animal model. Journal of Neurosurgery, 129(4), 997-1007. https://doi.org/10.3171/2017.5.JNS162579