Direct electron transfer of cytochrome C and its electrocatalytic properties on multiwalled carbon nanotubes/ciprofloxacin films

S. Ashok Kumar, Sea Fue Wang, Chun Ting Yeh, His Chuan Lu, Jen Chang Yang, Yu Tsern Chang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

In this study, stable and homogenous thin films of multiwalled carbon nanotubes (MWCNTs) were obtained on conducting surface using ciprofloxacin (CF, fluoroquinolone antibiotic) as an effective-dispersing agent. Further, MWCNTs/CF film modified electrodes (glassy carbon and indium tin oxide-coated glass electrode) are used successfully to study the direct electrochemistry of proteins. Here, cytochrome C (Cyt-C) was used as a model protein for investigation. A MWCNTs/CF film modified electrode was used as a biocompatible material for immobilization of Cyt-C from a neutral buffer solution (pH 7.2) using cyclic voltammetry (CV). Interestingly, Cyt-C retained its native state on the MWCNTs/CF film. The Cyt-C adsorbed MWCNTs/CF film was characterized by scanning electron microscopy (SEM), UV-visible spectrophotometry (UV-vis) and CV. SEM images showed the evidence for the adsorption of Cyt-C on the MWCNTs/CF film, and UV-vis spectrum confirmed that Cyt-C was in its native state on MWCNTs/CF film. Using CV, it was found that the electrochemical signal of Cyt-C was highly stable in the neutral buffer solution and its redox peak potential was pH dependent. The formal potential (-0.27 V) and electron transfer rate constant (13±1 s -1) were calculated for Cyt-C on MWCNTs/CF film modified electrode. A potential application of the Cyt-C/MWCNTs/CF electrode as a biosensor to monitor H 2O 2 has been investigated. The steady-state current response increases linearly with H 2O 2 concentration from 2×10 -6 to 7.8×10 -5 M. The detection limit for determination of H 2O 2 has been found to be 1.0×10 -6 M (S/N=3). Thus, Cyt-C/MWCNTs/CF film modified electrode can be used as a biosensing material for sensor applications.

Original languageEnglish
Pages (from-to)2129-2135
Number of pages7
JournalJournal of Solid State Electrochemistry
Volume14
Issue number11
DOIs
Publication statusPublished - Nov 2010

Keywords

  • Biosensor
  • Carbon nanotubes
  • Cytochrome C
  • Hydrogen peroxide sensor

ASJC Scopus subject areas

  • Electrochemistry
  • Electrical and Electronic Engineering
  • Condensed Matter Physics
  • Materials Science(all)

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