Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies

Ta Chun Cheng, Chu Hsiang Pan, Chien Shu Chen, Kuo Hsiang Chuang, Chih Hung Chuang, Chien Chaio Huang, Yu Yi Chu, Ya Chun Yang, Pei Yu Chu, Chien Han Kao, Yuan Chin Hsieh, Tian Lu Cheng

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The envelope glycoprotein E2 of classical swine fever virus (CSFV) is widely used as a marker for measuring vaccine efficacy and antibody titer. The glycosylation profile of E2 may affect the immunogenicity of the vaccine and the timing of re-vaccination. In this study, a human embryonic kidney cell line was used to secrete fully-glycosylated CSFV E2, which was then coated onto ELISA plates without purification or adjustment.The resulting E2-secreting medium-direct-coating (E2-mDc) ELISA was successfully used to measure anti-E2 antibody titers in vaccinated and field pig sera samples. Compared with a virus neutralization test (as standard), the E2-mDc ELISA was found to be more accurate (90%) than a commercial CSFV antibody diagnostic kit (62%). In conclusion, the mammalian cell-secreted antigen can provide cheap, accurate and effective assays for vaccine efficacy and disease diagnoses.

Original languageEnglish
Pages (from-to)107-109
Number of pages3
JournalVeterinary Journal
Volume205
Issue number1
DOIs
Publication statusPublished - Jul 1 2015

Fingerprint

Classical swine fever virus
coatings
Culture Media
culture media
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
vaccines
antigens
antibodies
Antibodies
Marker Vaccines
Neutralization Tests
analytical kits
neutralization tests
disease diagnosis
cells
kidney cells
glycosylation
Glycosylation
glycoproteins

Keywords

  • Classical swine fever virus
  • E2
  • Glycosylation
  • Mammalian expression
  • Medium-direct-coating ELISA

ASJC Scopus subject areas

  • Animal Science and Zoology
  • veterinary(all)

Cite this

Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies. / Cheng, Ta Chun; Pan, Chu Hsiang; Chen, Chien Shu; Chuang, Kuo Hsiang; Chuang, Chih Hung; Huang, Chien Chaio; Chu, Yu Yi; Yang, Ya Chun; Chu, Pei Yu; Kao, Chien Han; Hsieh, Yuan Chin; Cheng, Tian Lu.

In: Veterinary Journal, Vol. 205, No. 1, 01.07.2015, p. 107-109.

Research output: Contribution to journalArticle

Cheng, TC, Pan, CH, Chen, CS, Chuang, KH, Chuang, CH, Huang, CC, Chu, YY, Yang, YC, Chu, PY, Kao, CH, Hsieh, YC & Cheng, TL 2015, 'Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies', Veterinary Journal, vol. 205, no. 1, pp. 107-109. https://doi.org/10.1016/j.tvjl.2015.02.007
Cheng, Ta Chun ; Pan, Chu Hsiang ; Chen, Chien Shu ; Chuang, Kuo Hsiang ; Chuang, Chih Hung ; Huang, Chien Chaio ; Chu, Yu Yi ; Yang, Ya Chun ; Chu, Pei Yu ; Kao, Chien Han ; Hsieh, Yuan Chin ; Cheng, Tian Lu. / Direct coating of culture medium from cells secreting classical swine fever virus E2 antigen on ELISA plates for detection of E2-specific antibodies. In: Veterinary Journal. 2015 ; Vol. 205, No. 1. pp. 107-109.
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