Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees

C. H. Chou, C. H. Lee, L. S. Lu, I. W. Song, H. P. Chuang, S. Y. Kuo, J. Y. Wu, Y. T. Chen, V. B. Kraus, C. C. Wu, M. T M Lee

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Objective: To evaluate the interaction of articular cartilage (AC) and subchondral bone (SB) through analysis of osteoarthritis (OA)-related genes of site-matched tissue. Design: We developed a novel method for isolating site-matched overlying AC and underlying SB from three and four regions of interest respectively from the human knee tibial plateau (n = 50). For each site, the severity of cartilage changes of OA were assessed histologically, and the severity of bone abnormalities were assessed by microcomputed tomography. An RNA isolation procedure was optimized that yielded high quality RNA from site-matched AC and SB tibial regions. Quantitative polymerase chain reaction (Q-PCR) analysis was performed to evaluate gene expression of 61 OA-associated genes for correlation with cartilage integrity and bone structure parameters. Results: A total of 27 (44%) genes were coordinately up- or down-regulated in both tissues. The expression levels of 19 genes were statistically significantly correlated with the severity of AC degeneration and changes of SB structure; these included: ADAMTS1, ASPN, BMP6, BMPER, CCL2, CCL8, COL5A1, COL6A3, COL7A1, COL16A1, FRZB, GDF10, MMP3, OGN, OMD, POSTN, PTGES, TNFSF11 and WNT1. Conclusions: These results provide a strategy for identifying targets whose modification may have the potential to ameliorate pathological alterations and progression of disease in both AC and SB simultaneously. In addition, this is the first study, to our knowledge, to overcome the major difficulties related to isolation of high quality RNA from site-matched joint tissues. We expect this method to facilitate advances in our understanding of the coordinated molecular responses of the whole joint organ.

Original languageEnglish
Pages (from-to)450-461
Number of pages12
JournalOsteoarthritis and Cartilage
Volume21
Issue number3
DOIs
Publication statusPublished - Mar 2013

Fingerprint

Cartilage
Articular Cartilage
Gene expression
Knee
Bone
Gene Expression
Bone and Bones
Genes
RNA
Osteoarthritis
Tissue
Joints
X-Ray Microtomography
Polymerase chain reaction
Tomography
Disease Progression
Polymerase Chain Reaction

Keywords

  • Bone sectioning and grinding
  • Cartilage
  • Gene expression
  • Osteoarthritis
  • RNA isolation
  • Subchondral bone

ASJC Scopus subject areas

  • Biomedical Engineering
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees. / Chou, C. H.; Lee, C. H.; Lu, L. S.; Song, I. W.; Chuang, H. P.; Kuo, S. Y.; Wu, J. Y.; Chen, Y. T.; Kraus, V. B.; Wu, C. C.; Lee, M. T M.

In: Osteoarthritis and Cartilage, Vol. 21, No. 3, 03.2013, p. 450-461.

Research output: Contribution to journalArticle

Chou, C. H. ; Lee, C. H. ; Lu, L. S. ; Song, I. W. ; Chuang, H. P. ; Kuo, S. Y. ; Wu, J. Y. ; Chen, Y. T. ; Kraus, V. B. ; Wu, C. C. ; Lee, M. T M. / Direct assessment of articular cartilage and underlying subchondral bone reveals a progressive gene expression change in human osteoarthritic knees. In: Osteoarthritis and Cartilage. 2013 ; Vol. 21, No. 3. pp. 450-461.
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AU - Song, I. W.

AU - Chuang, H. P.

AU - Kuo, S. Y.

AU - Wu, J. Y.

AU - Chen, Y. T.

AU - Kraus, V. B.

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AU - Lee, M. T M

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AB - Objective: To evaluate the interaction of articular cartilage (AC) and subchondral bone (SB) through analysis of osteoarthritis (OA)-related genes of site-matched tissue. Design: We developed a novel method for isolating site-matched overlying AC and underlying SB from three and four regions of interest respectively from the human knee tibial plateau (n = 50). For each site, the severity of cartilage changes of OA were assessed histologically, and the severity of bone abnormalities were assessed by microcomputed tomography. An RNA isolation procedure was optimized that yielded high quality RNA from site-matched AC and SB tibial regions. Quantitative polymerase chain reaction (Q-PCR) analysis was performed to evaluate gene expression of 61 OA-associated genes for correlation with cartilage integrity and bone structure parameters. Results: A total of 27 (44%) genes were coordinately up- or down-regulated in both tissues. The expression levels of 19 genes were statistically significantly correlated with the severity of AC degeneration and changes of SB structure; these included: ADAMTS1, ASPN, BMP6, BMPER, CCL2, CCL8, COL5A1, COL6A3, COL7A1, COL16A1, FRZB, GDF10, MMP3, OGN, OMD, POSTN, PTGES, TNFSF11 and WNT1. Conclusions: These results provide a strategy for identifying targets whose modification may have the potential to ameliorate pathological alterations and progression of disease in both AC and SB simultaneously. In addition, this is the first study, to our knowledge, to overcome the major difficulties related to isolation of high quality RNA from site-matched joint tissues. We expect this method to facilitate advances in our understanding of the coordinated molecular responses of the whole joint organ.

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