Direct and indirect effects of ceramic far infrared radiation on the hydrogen peroxide-scavenging capacity and on murine macrophages under oxidative stress

Ting-Kai Leung, Yung Sheng Lin, Chi-Ming Lee, Yen Chou Chen, Huey Fang Shang, Sheng Yi Hsiao, Hsuan Tang Chang, Jo Shui Chao

Research output: Contribution to journalArticle

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Abstract

Far infrared (FIR) rays are used for many therapeutic purposes, but the intracellular mechanisms of their beneficial effects have not been entirely elucidated. The purposes of this study were thus to explore the effects of ceramic-generated far infrared ray (cFIR) on RAW 264.7 cells by determining the scavenging activity of hydrogen peroxide (H 2O 2), cell viability, and changes in cytochrome c levels and the NADP +/NADPH ratios. The results showed that the H 2O 2-scavenging activity directly increased by 10.26% after FIR application. Additional FIR treatment resulted in increased viability of murine macrophages with different concentrations of H 2O 2. cFIR significantly inhibited intracellular peroxide levels and LPS-induced peroxide production by macrophages. The increased ratio of hypodiploid cells elicited by H 2O 2 was significantly reduced by cFIR. The effects of cFIR on H 2O 2 toxicity were determined by measuring intracellular changes in cytochrome c levels and the ratio of NADP +/NADPH, and results showed that cFIR may block ROS-mediated cytotoxicity. In conclusion, data from this study suggest that cFIR may possess antiapoptotic effects by reducing ROS production by macrophages. We also review past articles related to the effects of oxidative stress from metabolically produced H 2O 2, and discuss possible beneficial effects of cFIR on living tissues.

Original languageEnglish
Pages (from-to)345-351
Number of pages7
JournalJournal of Medical and Biological Engineering
Volume31
Issue number5
DOIs
StatePublished - 2011

Fingerprint

Infrared Rays
Ceramics
Hydrogen Peroxide
Oxidative Stress
Macrophages
Radiation
Infrared radiation
NADP
Scavenging
Peroxides
Cytochromes c
Oxidative stress
Hydrogen peroxide
Proteins
Cell Survival
Cytotoxicity
Toxicity
Cells
Tissue
Hydrogen

Keywords

  • Ceramic-generated far infrared ray (cFIR)
  • Cytochrome c
  • Hydrogen peroxide (H O ) scavenging
  • Hypodiploid cell
  • Murine macrophages
  • Oxidative stress

ASJC Scopus subject areas

  • Biomedical Engineering
  • Medicine(all)

Cite this

Direct and indirect effects of ceramic far infrared radiation on the hydrogen peroxide-scavenging capacity and on murine macrophages under oxidative stress. / Leung, Ting-Kai; Lin, Yung Sheng; Lee, Chi-Ming; Chen, Yen Chou; Shang, Huey Fang; Hsiao, Sheng Yi; Chang, Hsuan Tang; Chao, Jo Shui.

In: Journal of Medical and Biological Engineering, Vol. 31, No. 5, 2011, p. 345-351.

Research output: Contribution to journalArticle

Leung, Ting-Kai; Lin, Yung Sheng; Lee, Chi-Ming; Chen, Yen Chou; Shang, Huey Fang; Hsiao, Sheng Yi; Chang, Hsuan Tang; Chao, Jo Shui / Direct and indirect effects of ceramic far infrared radiation on the hydrogen peroxide-scavenging capacity and on murine macrophages under oxidative stress.

In: Journal of Medical and Biological Engineering, Vol. 31, No. 5, 2011, p. 345-351.

Research output: Contribution to journalArticle

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