Abstract

Dipyridamole (persantin) is a nucleoside transport inhibitor and a non-specific phosphodiesterase inhibitor that increases intracellular levels of cAMP and cGMP through phosphodiesterase inhibition. Dipyridamole has been demonstrated to have an antiproliferative effect in glomerular mesangial cells. In the present study, we have confirmed that exposure of the mesangial cells to dipyridamole decreases the number of viable cells, as demonstrated by MTT assay. Dipyridamole suppressed [(superscript 3)H] thymidine incorporation into DNA and the reduction of viable cells are not due to toxicity because the LDH release from mesangial cells does not increase. Treatment of mesangial cells with dipyridamole arrests cell-cycle progression and increases the cell population at the sub G1 phase. Furthermore, incubation of mesangial cells with dipyridamole for 48 h induces characteristic features of apoptosis. The induction of mesangial cell apoptosis is correlated with Akt/PKB dephosphorylation and Bcl-2 down regulation. These data suggest that dipyridamole may block Akt/PKB phosphorylation and play a crucial role in the induction of apoptosis in rat mesangial cells.
Original languageEnglish
Pages (from-to)57-62
Number of pages6
JournalActa Nephrologica
Volume17
Issue number2
Publication statusPublished - 2003

Fingerprint

Mesangial Cells
Dipyridamole
Apoptosis
Phosphodiesterase Inhibitors
Phosphoric Diester Hydrolases
G1 Phase
Cell Cycle Checkpoints
Nucleosides
Thymidine
Down-Regulation
Cell Count
Phosphorylation
DNA

Keywords

  • Dipyridamole
  • 腎絲球環間膜細胞
  • 細胞凋亡
  • Akt/PKB
  • Mesangial cells
  • Apoptosis

Cite this

Dipyridamole Induces Apoptosis by Inhibiting Akt Activation in Rat Mesangial Cells. / Tso-Hsiao Chen; Yu-Ju Chen, ; Yung-Ho Hsu, ; Yuh-Mou Sue; Chun-Cheng Hou, ; Horng-Mo Lee.

In: Acta Nephrologica, Vol. 17, No. 2, 2003, p. 57-62.

Research output: Contribution to journalArticle

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abstract = "Dipyridamole (persantin) is a nucleoside transport inhibitor and a non-specific phosphodiesterase inhibitor that increases intracellular levels of cAMP and cGMP through phosphodiesterase inhibition. Dipyridamole has been demonstrated to have an antiproliferative effect in glomerular mesangial cells. In the present study, we have confirmed that exposure of the mesangial cells to dipyridamole decreases the number of viable cells, as demonstrated by MTT assay. Dipyridamole suppressed [(superscript 3)H] thymidine incorporation into DNA and the reduction of viable cells are not due to toxicity because the LDH release from mesangial cells does not increase. Treatment of mesangial cells with dipyridamole arrests cell-cycle progression and increases the cell population at the sub G1 phase. Furthermore, incubation of mesangial cells with dipyridamole for 48 h induces characteristic features of apoptosis. The induction of mesangial cell apoptosis is correlated with Akt/PKB dephosphorylation and Bcl-2 down regulation. These data suggest that dipyridamole may block Akt/PKB phosphorylation and play a crucial role in the induction of apoptosis in rat mesangial cells.",
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T1 - Dipyridamole Induces Apoptosis by Inhibiting Akt Activation in Rat Mesangial Cells

AU - Tso-Hsiao Chen, null

AU - Yu-Ju Chen, null

AU - Yung-Ho Hsu, null

AU - Yuh-Mou Sue, null

AU - Chun-Cheng Hou, null

AU - Horng-Mo Lee, null

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N2 - Dipyridamole (persantin) is a nucleoside transport inhibitor and a non-specific phosphodiesterase inhibitor that increases intracellular levels of cAMP and cGMP through phosphodiesterase inhibition. Dipyridamole has been demonstrated to have an antiproliferative effect in glomerular mesangial cells. In the present study, we have confirmed that exposure of the mesangial cells to dipyridamole decreases the number of viable cells, as demonstrated by MTT assay. Dipyridamole suppressed [(superscript 3)H] thymidine incorporation into DNA and the reduction of viable cells are not due to toxicity because the LDH release from mesangial cells does not increase. Treatment of mesangial cells with dipyridamole arrests cell-cycle progression and increases the cell population at the sub G1 phase. Furthermore, incubation of mesangial cells with dipyridamole for 48 h induces characteristic features of apoptosis. The induction of mesangial cell apoptosis is correlated with Akt/PKB dephosphorylation and Bcl-2 down regulation. These data suggest that dipyridamole may block Akt/PKB phosphorylation and play a crucial role in the induction of apoptosis in rat mesangial cells.

AB - Dipyridamole (persantin) is a nucleoside transport inhibitor and a non-specific phosphodiesterase inhibitor that increases intracellular levels of cAMP and cGMP through phosphodiesterase inhibition. Dipyridamole has been demonstrated to have an antiproliferative effect in glomerular mesangial cells. In the present study, we have confirmed that exposure of the mesangial cells to dipyridamole decreases the number of viable cells, as demonstrated by MTT assay. Dipyridamole suppressed [(superscript 3)H] thymidine incorporation into DNA and the reduction of viable cells are not due to toxicity because the LDH release from mesangial cells does not increase. Treatment of mesangial cells with dipyridamole arrests cell-cycle progression and increases the cell population at the sub G1 phase. Furthermore, incubation of mesangial cells with dipyridamole for 48 h induces characteristic features of apoptosis. The induction of mesangial cell apoptosis is correlated with Akt/PKB dephosphorylation and Bcl-2 down regulation. These data suggest that dipyridamole may block Akt/PKB phosphorylation and play a crucial role in the induction of apoptosis in rat mesangial cells.

KW - Dipyridamole

KW - 腎絲球環間膜細胞

KW - 細胞凋亡

KW - Akt/PKB

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KW - Apoptosis

M3 - Article

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JO - Acta Nephrologica

JF - Acta Nephrologica

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