Dimethyl Sulfoxide Attenuates Acute Lung Injury Induced by Hemorrhagic Shock/Resuscitation in Rats

Yu Chi Tsung, Chih Yang Chung, Hung Chieh Wan, Ya Ying Chang, Ping Cheng Shih, Han Shui Hsu, Ming Chang Kao, Chun Jen Huang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Inflammation following hemorrhagic shock/resuscitation (HS/RES) induces acute lung injury (ALI). Dimethyl sulfoxide (DMSO) possesses anti-inflammatory and antioxidative capacities. We sought to clarify whether DMSO could attenuate ALI induced by HS/RES. Male Sprague-Dawley rats were allocated to receive either a sham operation, sham plus DMSO, HS/RES, or HS/RES plus DMSO, and these were denoted as the Sham, Sham + DMSO, HS/RES, or HS/RES + DMSO group, respectively (n = 12 in each group). HS/RES was achieved by drawing blood to lower mean arterial pressure (40–45 mmHg for 60 min) followed by reinfusion with shed blood/saline mixtures. All rats received an intravenous injection of normal saline or DMSO immediately before resuscitation or at matching points relative to the sham groups. Arterial blood gas and histological assays (including histopathology, neutrophil infiltration, and lung water content) confirmed that HS/RES induced ALI. Significant increases in pulmonary expression of tumor necrosis factor-α (TNF-α), malondialdehyde, nuclear factor-kappa B (NF-κB), inducible nitric oxide synthase (iNOS), and cyclooxygenase 2 (COX-2) confirmed that HS/RES induced pulmonary inflammation and oxidative stress. DMSO significantly attenuated the pulmonary inflammation and ALI induced by HS/RES. The mechanisms for this may involve reducing inflammation and oxidative stress through inhibition of pulmonary NF-κB, TNF-α, iNOS, and COX-2 expression.

Original languageEnglish
Pages (from-to)555-565
Number of pages11
JournalInflammation
Volume40
Issue number2
DOIs
Publication statusPublished - Apr 1 2017
Externally publishedYes

Fingerprint

Hemorrhagic Shock
Acute Lung Injury
Dimethyl Sulfoxide
Resuscitation
NF-kappa B
Nitric Oxide Synthase Type II
Cyclooxygenase 2
Lung
Pneumonia
Oxidative Stress
Tumor Necrosis Factor-alpha
Inflammation
Neutrophil Infiltration
Malondialdehyde
Intravenous Injections
Sprague Dawley Rats
Arterial Pressure
Anti-Inflammatory Agents
Gases

Keywords

  • COX-2
  • cytokine
  • inflammation
  • iNOS
  • NF-κB
  • TNF-α

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Dimethyl Sulfoxide Attenuates Acute Lung Injury Induced by Hemorrhagic Shock/Resuscitation in Rats. / Tsung, Yu Chi; Chung, Chih Yang; Wan, Hung Chieh; Chang, Ya Ying; Shih, Ping Cheng; Hsu, Han Shui; Kao, Ming Chang; Huang, Chun Jen.

In: Inflammation, Vol. 40, No. 2, 01.04.2017, p. 555-565.

Research output: Contribution to journalArticle

Tsung, YC, Chung, CY, Wan, HC, Chang, YY, Shih, PC, Hsu, HS, Kao, MC & Huang, CJ 2017, 'Dimethyl Sulfoxide Attenuates Acute Lung Injury Induced by Hemorrhagic Shock/Resuscitation in Rats', Inflammation, vol. 40, no. 2, pp. 555-565. https://doi.org/10.1007/s10753-016-0502-4
Tsung, Yu Chi ; Chung, Chih Yang ; Wan, Hung Chieh ; Chang, Ya Ying ; Shih, Ping Cheng ; Hsu, Han Shui ; Kao, Ming Chang ; Huang, Chun Jen. / Dimethyl Sulfoxide Attenuates Acute Lung Injury Induced by Hemorrhagic Shock/Resuscitation in Rats. In: Inflammation. 2017 ; Vol. 40, No. 2. pp. 555-565.
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