Differentiation of pheochromocytoma PC 12 cells induced by human urine extract and the involvement of the extracellular signal-regulated kinase signaling pathway

Chih Jung Yao, Gi Ming Lai, Chin Feng Chan, Ya Yu Yang, Frank C. Liu, Shuang En Chuang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Objective: The aim of this study was to investigate the effects of a human urine preparation on the differentiation of tumor cells. Design: The pheochromocytoma PC12 cells were used to examine the effects of a human urine preparation, CDA-2 on the induction of differentiation markers, neurofilaments, and compared with that induced by nerve growth factor (NGF). The MAPK/ERK kinase (MEK) inhibitor U0126 was used to examine the involvement of mitogen-activated protein kinase (MAPK) signaling pathway in this differentiation inducing effect. Results: We find that CDA-2 could induce differentiation of pheochromocytoma PC12 cells, as evidenced by the markedly increased expression of neurofilaments to a level comparable to those induced by NGF. This phenomenon was accompanied by the phosphorylation of extracellular-signal-regulated kinase (ERK) and could be inhibited by the MEK inhibitor, U0126. Conclusions: Our results demonstrate the presence of active components in the human urine extract that can induce differentiation of PC12 cells and may involve the ERK signaling pathway. This may provide new insights for seeking novel differentiation agents and offer hope for cancer patients.

Original languageEnglish
Pages (from-to)903-908
Number of pages6
JournalJournal of Alternative and Complementary Medicine
Volume11
Issue number5
DOIs
Publication statusPublished - Oct 2005
Externally publishedYes

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PC12 Cells
Extracellular Signal-Regulated MAP Kinases
Pheochromocytoma
Intermediate Filaments
Urine
Nerve Growth Factor
Mitogen-Activated Protein Kinases
Differentiation Antigens
Mitogen-Activated Protein Kinase Kinases
Cell Differentiation
Neoplasms
Phosphotransferases
Phosphorylation
U 0126

ASJC Scopus subject areas

  • Medicine(all)
  • Complementary and alternative medicine
  • Nursing(all)

Cite this

Differentiation of pheochromocytoma PC 12 cells induced by human urine extract and the involvement of the extracellular signal-regulated kinase signaling pathway. / Yao, Chih Jung; Lai, Gi Ming; Chan, Chin Feng; Yang, Ya Yu; Liu, Frank C.; Chuang, Shuang En.

In: Journal of Alternative and Complementary Medicine, Vol. 11, No. 5, 10.2005, p. 903-908.

Research output: Contribution to journalArticle

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AU - Yang, Ya Yu

AU - Liu, Frank C.

AU - Chuang, Shuang En

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