Differential regulation of antigen-induced IL-4 and IL-13 generation from T lymphocytes by IFN-α

David M. Essayan, Guha Krishnaswamy, Alfonso Oriente, Lawrence M. Lichtenstein, Shau Ku Huang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Background: IL-4 and IL-13 are related cytokines with similar functional properties. Differential regulation of IL-4 and IL-13 has not been described. Objective: We have examined the effects of IFN-α on antigen-driven proliferation, IL-4 generation, and IL-13 generation from human PBMCs and T-cell clones. Methods: Proliferation was assessed by 3H-thymidine incorporation. Cytokine generation was assessed by reverse transcription PCR and ELISA. Messenger RNA stability was assessed in the presence of actinomycin D. Results: IFN-α induced a concentration-dependent inhibition of antigen-driven proliferation of TH1 and TH2 clones (median effective concentration, 150 to 200 U/mL); the sensitivity of TH1 and TH2 clones to IFN-α was not significantly different (P = .6). IFN-α induced an analogous concentration-dependent inhibition of antigen-driven IL-13 generation from TH1 and TH2 clones (median effective concentration, 100 U/mL); this effect was evident by 12 hours of culture and persisted beyond 48 hours. However, IL-4 generation from TH2 clones was insensitive to IFN-α at all concentrations and times tested (1 to 10,000 U/mL). A similar inhibitory effect of IFN-α on mitogen-driven proliferation and IL-13 generation from PBMCs was demonstrated; once again, IL-4 generation from PBMCs was insensitive to IFN-α. IL-13 mRNA stability was unaffected by IFN-α, suggesting transcriptional regulation. Conclusion: IFN-α differentially regulates antigen-stimulated IL-4 and IL-13 generation.

Original languageEnglish
Pages (from-to)451-457
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume103
Issue number3 II
Publication statusPublished - Dec 1 1999
Externally publishedYes

Fingerprint

Interleukin-13
Interleukin-4
T-Lymphocytes
Antigens
Clone Cells
RNA Stability
Cytokines
Dactinomycin
Mitogens
Thymidine
Reverse Transcription
Enzyme-Linked Immunosorbent Assay
Polymerase Chain Reaction
Messenger RNA

Keywords

  • Cytokine
  • Human
  • IFN-α
  • IL-13
  • IL-4
  • T lymphocyte

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Differential regulation of antigen-induced IL-4 and IL-13 generation from T lymphocytes by IFN-α. / Essayan, David M.; Krishnaswamy, Guha; Oriente, Alfonso; Lichtenstein, Lawrence M.; Huang, Shau Ku.

In: Journal of Allergy and Clinical Immunology, Vol. 103, No. 3 II, 01.12.1999, p. 451-457.

Research output: Contribution to journalArticle

Essayan, DM, Krishnaswamy, G, Oriente, A, Lichtenstein, LM & Huang, SK 1999, 'Differential regulation of antigen-induced IL-4 and IL-13 generation from T lymphocytes by IFN-α', Journal of Allergy and Clinical Immunology, vol. 103, no. 3 II, pp. 451-457.
Essayan, David M. ; Krishnaswamy, Guha ; Oriente, Alfonso ; Lichtenstein, Lawrence M. ; Huang, Shau Ku. / Differential regulation of antigen-induced IL-4 and IL-13 generation from T lymphocytes by IFN-α. In: Journal of Allergy and Clinical Immunology. 1999 ; Vol. 103, No. 3 II. pp. 451-457.
@article{e12ce51185824f149b58f9db52bc6c70,
title = "Differential regulation of antigen-induced IL-4 and IL-13 generation from T lymphocytes by IFN-α",
abstract = "Background: IL-4 and IL-13 are related cytokines with similar functional properties. Differential regulation of IL-4 and IL-13 has not been described. Objective: We have examined the effects of IFN-α on antigen-driven proliferation, IL-4 generation, and IL-13 generation from human PBMCs and T-cell clones. Methods: Proliferation was assessed by 3H-thymidine incorporation. Cytokine generation was assessed by reverse transcription PCR and ELISA. Messenger RNA stability was assessed in the presence of actinomycin D. Results: IFN-α induced a concentration-dependent inhibition of antigen-driven proliferation of TH1 and TH2 clones (median effective concentration, 150 to 200 U/mL); the sensitivity of TH1 and TH2 clones to IFN-α was not significantly different (P = .6). IFN-α induced an analogous concentration-dependent inhibition of antigen-driven IL-13 generation from TH1 and TH2 clones (median effective concentration, 100 U/mL); this effect was evident by 12 hours of culture and persisted beyond 48 hours. However, IL-4 generation from TH2 clones was insensitive to IFN-α at all concentrations and times tested (1 to 10,000 U/mL). A similar inhibitory effect of IFN-α on mitogen-driven proliferation and IL-13 generation from PBMCs was demonstrated; once again, IL-4 generation from PBMCs was insensitive to IFN-α. IL-13 mRNA stability was unaffected by IFN-α, suggesting transcriptional regulation. Conclusion: IFN-α differentially regulates antigen-stimulated IL-4 and IL-13 generation.",
keywords = "Cytokine, Human, IFN-α, IL-13, IL-4, T lymphocyte",
author = "Essayan, {David M.} and Guha Krishnaswamy and Alfonso Oriente and Lichtenstein, {Lawrence M.} and Huang, {Shau Ku}",
year = "1999",
month = "12",
day = "1",
language = "English",
volume = "103",
pages = "451--457",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "3 II",

}

TY - JOUR

T1 - Differential regulation of antigen-induced IL-4 and IL-13 generation from T lymphocytes by IFN-α

AU - Essayan, David M.

AU - Krishnaswamy, Guha

AU - Oriente, Alfonso

AU - Lichtenstein, Lawrence M.

AU - Huang, Shau Ku

PY - 1999/12/1

Y1 - 1999/12/1

N2 - Background: IL-4 and IL-13 are related cytokines with similar functional properties. Differential regulation of IL-4 and IL-13 has not been described. Objective: We have examined the effects of IFN-α on antigen-driven proliferation, IL-4 generation, and IL-13 generation from human PBMCs and T-cell clones. Methods: Proliferation was assessed by 3H-thymidine incorporation. Cytokine generation was assessed by reverse transcription PCR and ELISA. Messenger RNA stability was assessed in the presence of actinomycin D. Results: IFN-α induced a concentration-dependent inhibition of antigen-driven proliferation of TH1 and TH2 clones (median effective concentration, 150 to 200 U/mL); the sensitivity of TH1 and TH2 clones to IFN-α was not significantly different (P = .6). IFN-α induced an analogous concentration-dependent inhibition of antigen-driven IL-13 generation from TH1 and TH2 clones (median effective concentration, 100 U/mL); this effect was evident by 12 hours of culture and persisted beyond 48 hours. However, IL-4 generation from TH2 clones was insensitive to IFN-α at all concentrations and times tested (1 to 10,000 U/mL). A similar inhibitory effect of IFN-α on mitogen-driven proliferation and IL-13 generation from PBMCs was demonstrated; once again, IL-4 generation from PBMCs was insensitive to IFN-α. IL-13 mRNA stability was unaffected by IFN-α, suggesting transcriptional regulation. Conclusion: IFN-α differentially regulates antigen-stimulated IL-4 and IL-13 generation.

AB - Background: IL-4 and IL-13 are related cytokines with similar functional properties. Differential regulation of IL-4 and IL-13 has not been described. Objective: We have examined the effects of IFN-α on antigen-driven proliferation, IL-4 generation, and IL-13 generation from human PBMCs and T-cell clones. Methods: Proliferation was assessed by 3H-thymidine incorporation. Cytokine generation was assessed by reverse transcription PCR and ELISA. Messenger RNA stability was assessed in the presence of actinomycin D. Results: IFN-α induced a concentration-dependent inhibition of antigen-driven proliferation of TH1 and TH2 clones (median effective concentration, 150 to 200 U/mL); the sensitivity of TH1 and TH2 clones to IFN-α was not significantly different (P = .6). IFN-α induced an analogous concentration-dependent inhibition of antigen-driven IL-13 generation from TH1 and TH2 clones (median effective concentration, 100 U/mL); this effect was evident by 12 hours of culture and persisted beyond 48 hours. However, IL-4 generation from TH2 clones was insensitive to IFN-α at all concentrations and times tested (1 to 10,000 U/mL). A similar inhibitory effect of IFN-α on mitogen-driven proliferation and IL-13 generation from PBMCs was demonstrated; once again, IL-4 generation from PBMCs was insensitive to IFN-α. IL-13 mRNA stability was unaffected by IFN-α, suggesting transcriptional regulation. Conclusion: IFN-α differentially regulates antigen-stimulated IL-4 and IL-13 generation.

KW - Cytokine

KW - Human

KW - IFN-α

KW - IL-13

KW - IL-4

KW - T lymphocyte

UR - http://www.scopus.com/inward/record.url?scp=0033051613&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033051613&partnerID=8YFLogxK

M3 - Article

VL - 103

SP - 451

EP - 457

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 3 II

ER -