Differential inhibition of reverse transcriptase and cellular DNA polymerase-α activities by lignans isolated from Chinese herbs, Phyllanthus myrtifolius Moon, and tannins from Lonicera japonica Thunb and Castanopsis hystrix

Chia Wen Chang, Mei Tsu Lin, Shoei Sheng Lee, Karin C S Chen Liu, Feng Lin Hsu, Jung Yaw Lin

Research output: Contribution to journalReview article

107 Citations (Scopus)

Abstract

Two lignans, phyllamycin B and retrojusticidin B isolated from Phyllanthus myrtifolius Moon have been demonstrated to have a strong inhibitory effect on human immunodeficiency virus-1 reverse transcriptase activity (HIV-1 RT), but much less inhibitory effect on human DNA polymerase-α (HDNAP-α) activity. Fifty percent inhibitory concentrations of phyllamycin B and retrojusticidin B were determined to be 3.5 and 5.5 μM for HIV-1 RT, and 289 and 989 μM for HDNAP-α, respectively. The mode of inhibition was found to be non-competitive inhibition with respect to template-primer and triphosphate substrate. Several tannins such as caffeoylquinates (CQs) isolated from Lonicera japonica Thunb, galloylquinates (GQs) and galloylshikimates (GSs) purified from Castanopsis hystrix were shown to have a much less selective inhibitory effect on HIV-1 RT.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalAntiviral Research
Volume27
Issue number4
DOIs
Publication statusPublished - 1995

Keywords

  • Caffeoylquinate
  • Galloylquinate
  • Galloylshikimate
  • Lignan
  • Reverse transcriptase inhibition

ASJC Scopus subject areas

  • Virology
  • Pharmacology

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