Differential expression profiling between atypical teratoid/rhabdoid and medulloblastoma tumor in vitro and in vivo using microarray analysis

Hsin I. Ma, Chung Lan Kao, Yi Yen Lee, Guang Yuh Chiou, Lung Kuo Tai, Kai Hsi Lu, Chi Shuan Huang, Yi Wei Chen, Shih Hwa Chiou, Ing Chan Cheng, Tai-Tong Wong

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Objectives: Atypical teratoid/rhabdoid tumor (AT/RT) and medulloblastoma (MB) are the most malignant primary brain tumors in early childhood. AT/RT is frequently misdiagnosed as primitive neuroectodermal tumor/medulloblastoma. The biological features and clinical outcomes of AT/RT and MB are extremely different. In this study, we used microarray as a platform to distinguish these two tumors with the definitive diagnostic marker as well as the profiling of expression genes. Methods: In order to clarify the pathogenesis and find the biological markers for AT/RT, we established a derivative AT/RT primary cell culture. The differential profiling between AT/RT and MB were analyzed by using microarray method. Results: With the use of the microarray method, we demonstrated that 15 genes were significantly changed (at least 5-fold in upregulation and 1/5-fold in downregulation) between AT/RT and MB in tissues and cell lines. The quantitative reverse transcription-polymerase chain reaction analyses further confirmed that mRNA expression levels of SERPINI1 and osteopontin were highly expressed in AT/RT cells and tissues than those in MB. Importantly, our microarray result suggested that AT/RT presents the stemness-like pattern and expression profiling of embryonic stem cells as well as high mRNA expressions of Oct-4, Nanog, Sox-2, and c-Myc. Conclusions: Our study demonstrated the differential gene expression profiling between AT/RT and MB. Based on the microarray findings, AT/RTs present embryonic stem-like gene recapitulation and further provide novel insights into their underlying biology.

Original languageEnglish
Pages (from-to)293-303
Number of pages11
JournalChild's Nervous System
Volume26
Issue number3
DOIs
Publication statusPublished - Jan 1 2010
Externally publishedYes

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Medulloblastoma
Microarray Analysis
Gene Expression Profiling
Typical Teratoid Rhabdoid Tumor
Atypical Teratoid Tumor
In Vitro Techniques
Primitive Neuroectodermal Tumors
Messenger RNA
Osteopontin
Primary Cell Culture
Embryonic Stem Cells
Diagnostic Errors
Brain Neoplasms
Genes
Reverse Transcription
Up-Regulation
Down-Regulation
Biomarkers
Cell Line
Polymerase Chain Reaction

Keywords

  • Atypical teratoid/rhabdoid tumor (AT/RT)
  • Comparative genomic hybridization (CGH)
  • Medulloblastoma (MB)
  • Microarray

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Clinical Neurology

Cite this

Differential expression profiling between atypical teratoid/rhabdoid and medulloblastoma tumor in vitro and in vivo using microarray analysis. / Ma, Hsin I.; Kao, Chung Lan; Lee, Yi Yen; Chiou, Guang Yuh; Tai, Lung Kuo; Lu, Kai Hsi; Huang, Chi Shuan; Chen, Yi Wei; Chiou, Shih Hwa; Cheng, Ing Chan; Wong, Tai-Tong.

In: Child's Nervous System, Vol. 26, No. 3, 01.01.2010, p. 293-303.

Research output: Contribution to journalArticle

Ma, Hsin I. ; Kao, Chung Lan ; Lee, Yi Yen ; Chiou, Guang Yuh ; Tai, Lung Kuo ; Lu, Kai Hsi ; Huang, Chi Shuan ; Chen, Yi Wei ; Chiou, Shih Hwa ; Cheng, Ing Chan ; Wong, Tai-Tong. / Differential expression profiling between atypical teratoid/rhabdoid and medulloblastoma tumor in vitro and in vivo using microarray analysis. In: Child's Nervous System. 2010 ; Vol. 26, No. 3. pp. 293-303.
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AU - Ma, Hsin I.

AU - Kao, Chung Lan

AU - Lee, Yi Yen

AU - Chiou, Guang Yuh

AU - Tai, Lung Kuo

AU - Lu, Kai Hsi

AU - Huang, Chi Shuan

AU - Chen, Yi Wei

AU - Chiou, Shih Hwa

AU - Cheng, Ing Chan

AU - Wong, Tai-Tong

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N2 - Objectives: Atypical teratoid/rhabdoid tumor (AT/RT) and medulloblastoma (MB) are the most malignant primary brain tumors in early childhood. AT/RT is frequently misdiagnosed as primitive neuroectodermal tumor/medulloblastoma. The biological features and clinical outcomes of AT/RT and MB are extremely different. In this study, we used microarray as a platform to distinguish these two tumors with the definitive diagnostic marker as well as the profiling of expression genes. Methods: In order to clarify the pathogenesis and find the biological markers for AT/RT, we established a derivative AT/RT primary cell culture. The differential profiling between AT/RT and MB were analyzed by using microarray method. Results: With the use of the microarray method, we demonstrated that 15 genes were significantly changed (at least 5-fold in upregulation and 1/5-fold in downregulation) between AT/RT and MB in tissues and cell lines. The quantitative reverse transcription-polymerase chain reaction analyses further confirmed that mRNA expression levels of SERPINI1 and osteopontin were highly expressed in AT/RT cells and tissues than those in MB. Importantly, our microarray result suggested that AT/RT presents the stemness-like pattern and expression profiling of embryonic stem cells as well as high mRNA expressions of Oct-4, Nanog, Sox-2, and c-Myc. Conclusions: Our study demonstrated the differential gene expression profiling between AT/RT and MB. Based on the microarray findings, AT/RTs present embryonic stem-like gene recapitulation and further provide novel insights into their underlying biology.

AB - Objectives: Atypical teratoid/rhabdoid tumor (AT/RT) and medulloblastoma (MB) are the most malignant primary brain tumors in early childhood. AT/RT is frequently misdiagnosed as primitive neuroectodermal tumor/medulloblastoma. The biological features and clinical outcomes of AT/RT and MB are extremely different. In this study, we used microarray as a platform to distinguish these two tumors with the definitive diagnostic marker as well as the profiling of expression genes. Methods: In order to clarify the pathogenesis and find the biological markers for AT/RT, we established a derivative AT/RT primary cell culture. The differential profiling between AT/RT and MB were analyzed by using microarray method. Results: With the use of the microarray method, we demonstrated that 15 genes were significantly changed (at least 5-fold in upregulation and 1/5-fold in downregulation) between AT/RT and MB in tissues and cell lines. The quantitative reverse transcription-polymerase chain reaction analyses further confirmed that mRNA expression levels of SERPINI1 and osteopontin were highly expressed in AT/RT cells and tissues than those in MB. Importantly, our microarray result suggested that AT/RT presents the stemness-like pattern and expression profiling of embryonic stem cells as well as high mRNA expressions of Oct-4, Nanog, Sox-2, and c-Myc. Conclusions: Our study demonstrated the differential gene expression profiling between AT/RT and MB. Based on the microarray findings, AT/RTs present embryonic stem-like gene recapitulation and further provide novel insights into their underlying biology.

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KW - Microarray

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