Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

Hung I. Yeh; Yu J. Lai; Yi N. Lee; Yi J. Chen; Yao Chang Chen; Chih Chun Chen; Shih A. Chen; Cheng I. Lin; Cheng H. Tsai

Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

Hung I. Yeh, Yu J. Lai, Yi N. Lee, Yi J. Chen, Yao Chang Chen, Chih Chun Chen, Shih A. Chen, Cheng I. Lin, Cheng H. Tsai

Research output: Contribution to journal › Article › peer-review

24 Citations (Scopus)

Abstract

We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p

Original language	English
Pages (from-to)	259-266
Number of pages	8
Journal	Journal of Histochemistry and Cytochemistry
Volume	51
Issue number	2
Publication status	Published - Feb 1 2003

Keywords

Connexins
Gap junctions
Myocardial sleeve
Single cardiomyocytes

ASJC Scopus subject areas

Cell Biology
Anatomy

Cite this

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title = "Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins",

abstract = "We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p",

keywords = "Connexins, Gap junctions, Myocardial sleeve, Single cardiomyocytes",

author = "Yeh, {Hung I.} and Lai, {Yu J.} and Lee, {Yi N.} and Chen, {Yi J.} and Chen, {Yao Chang} and Chen, {Chih Chun} and Chen, {Shih A.} and Lin, {Cheng I.} and Tsai, {Cheng H.}",

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T1 - Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

AU - Yeh, Hung I.

AU - Lai, Yu J.

AU - Lee, Yi N.

AU - Chen, Yi J.

AU - Chen, Yao Chang

AU - Chen, Chih Chun

AU - Chen, Shih A.

AU - Lin, Cheng I.

AU - Tsai, Cheng H.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p

AB - We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p

KW - Connexins

KW - Gap junctions

KW - Myocardial sleeve

KW - Single cardiomyocytes

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ER -

Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

Abstract

Keywords

ASJC Scopus subject areas

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