Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

Hung I. Yeh, Yu J. Lai, Yi N. Lee, Yi J. Chen, Yao Chang Chen, Chih Chun Chen, Shih A. Chen, Cheng I. Lin, Cheng H. Tsai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume51
Issue number2
Publication statusPublished - Feb 1 2003

Keywords

  • Connexins
  • Gap junctions
  • Myocardial sleeve
  • Single cardiomyocytes

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

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    Yeh, H. I., Lai, Y. J., Lee, Y. N., Chen, Y. J., Chen, Y. C., Chen, C. C., Chen, S. A., Lin, C. I., & Tsai, C. H. (2003). Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins. Journal of Histochemistry and Cytochemistry, 51(2), 259-266.