Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

Hung I. Yeh, Yu J. Lai, Yi N. Lee, Yi J. Chen, Yao Chang Chen, Chih Chun Chen, Shih A. Chen, Cheng I. Lin, Cheng H. Tsai

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p

Original languageEnglish
Pages (from-to)259-266
Number of pages8
JournalJournal of Histochemistry and Cytochemistry
Volume51
Issue number2
Publication statusPublished - Feb 1 2003

Fingerprint

Connexin 43
Gap Junctions
Cardiac Myocytes
Canidae
Veins
Thorax
Superior Vena Cava
Vinculin
Actinin
Connexins
Pulmonary Veins
Immune Sera
Microscopy
Enzymes

Keywords

  • Connexins
  • Gap junctions
  • Myocardial sleeve
  • Single cardiomyocytes

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins. / Yeh, Hung I.; Lai, Yu J.; Lee, Yi N.; Chen, Yi J.; Chen, Yao Chang; Chen, Chih Chun; Chen, Shih A.; Lin, Cheng I.; Tsai, Cheng H.

In: Journal of Histochemistry and Cytochemistry, Vol. 51, No. 2, 01.02.2003, p. 259-266.

Research output: Contribution to journalArticle

Yeh, HI, Lai, YJ, Lee, YN, Chen, YJ, Chen, YC, Chen, CC, Chen, SA, Lin, CI & Tsai, CH 2003, 'Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins', Journal of Histochemistry and Cytochemistry, vol. 51, no. 2, pp. 259-266.
Yeh, Hung I. ; Lai, Yu J. ; Lee, Yi N. ; Chen, Yi J. ; Chen, Yao Chang ; Chen, Chih Chun ; Chen, Shih A. ; Lin, Cheng I. ; Tsai, Cheng H. / Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins. In: Journal of Histochemistry and Cytochemistry. 2003 ; Vol. 51, No. 2. pp. 259-266.
@article{0da9496da83f41b485fd5a834719a4e0,
title = "Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins",
abstract = "We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2{\%} vs 1.5 ± 0.2{\%}; p",
keywords = "Connexins, Gap junctions, Myocardial sleeve, Single cardiomyocytes",
author = "Yeh, {Hung I.} and Lai, {Yu J.} and Lee, {Yi N.} and Chen, {Yi J.} and Chen, {Yao Chang} and Chen, {Chih Chun} and Chen, {Shih A.} and Lin, {Cheng I.} and Tsai, {Cheng H.}",
year = "2003",
month = "2",
day = "1",
language = "English",
volume = "51",
pages = "259--266",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "2",

}

TY - JOUR

T1 - Differential expression of connexin43 gap junctions in cardiomyocytes isolated from canine thoracic veins

AU - Yeh, Hung I.

AU - Lai, Yu J.

AU - Lee, Yi N.

AU - Chen, Yi J.

AU - Chen, Yao Chang

AU - Chen, Chih Chun

AU - Chen, Shih A.

AU - Lin, Cheng I.

AU - Tsai, Cheng H.

PY - 2003/2/1

Y1 - 2003/2/1

N2 - We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p

AB - We investigated the phenotypic features of cardiomyocytes, including the gap junctions, in the myocardial sleeve of thoracic veins. Single cardiomyocytes, isolated from the canine pulmonary veins (PV) and superior vena cava (SVC) using digestive enzymes, were examined by immunoconfocal microscopy using antisera against connexin43 (Cx43), Cx40, and other cell markers. The results showed that isolated cardiomyocytes displayed rod shapes of various sizes, ranging from 200 μm in length, and all the cells expressed α-actinin and vinculin. Gap junctions made of various amounts of Cx43 and Cx40 were found at the cell borders. These two connexins were extensively co-localized. Comparison between the thoracic veins showed that cells of the SVC contained more Cx43 gap junctions (total Cx43 gap junctions area per cell surface area, 4.0 ± 0.2% vs 1.5 ± 0.2%; p

KW - Connexins

KW - Gap junctions

KW - Myocardial sleeve

KW - Single cardiomyocytes

UR - http://www.scopus.com/inward/record.url?scp=0037310449&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037310449&partnerID=8YFLogxK

M3 - Article

C2 - 12533535

AN - SCOPUS:0037310449

VL - 51

SP - 259

EP - 266

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 2

ER -