Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: Evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells

Chin Hsuan Hsieh, Shih Chieh Hsiung, Chi Tai Yeh, Chih Feng Yen, Yah Huei Wu Chou, Wei Yi Lei, See Tong Pang, Cheng Keng Chuang, Shuen Kuei Liao

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Epithelioid and fibroblastoid subsets coexist in the human sarcomatoid renal cell carcinoma (sRCC) cell line, RCC52, according to previous clonal studies. Herein, using monoclonal antibodies to CD44 and CD24 markers, we identified and isolated these two populations, and showed that CD44bright/CD24dim and CD44bright/CD24bright phenotypes correspond to epithelioid and fibroblastoid subsets, respectively. Both sorted subsets displayed different levels of tumorigenicity in xenotransplantation, indicating that each harbored its own cancer stem cells (CSCs). The CD44bright/CD24bright subset, associated with higher expression of MMP-7, -8 and TIMP-1 transcripts, showed greater migratory/invasive potential than the CD44bright/CD24dim subset, which was associated with higher expression of MMP-2, -9 and TIMP-2 transcripts. Both subsets differentially expressed stemness gene products c-Myc, Oct4A, Notch1, Notch2 and Notch3, and the RCC stem cell marker, CD105 in 4-5% of RCC52 cells. These results suggest the presence of CSCs in both sRCC subsets for the first time and should therefore be considered potential therapeutic targets for this aggressive malignancy.

Original languageEnglish
Pages (from-to)15593-15609
Number of pages17
JournalOncotarget
Volume8
Issue number9
DOIs
Publication statusPublished - Jan 1 2017

Fingerprint

Neoplastic Stem Cells
Matrix Metalloproteinases
Renal Cell Carcinoma
Carcinoma
Kidney
Tissue Inhibitor of Metalloproteinase-2
Cell Line
Heterologous Transplantation
myc Genes
Tissue Inhibitor of Metalloproteinase-1
Stem Cells
Monoclonal Antibodies
Phenotype
Population
Neoplasms
Therapeutics

Keywords

  • Cancer stem cells
  • CD24
  • CD44
  • Epithelioid and fibroblastoid subsets
  • Sarcomatoid renal cell carcinoma

ASJC Scopus subject areas

  • Oncology

Cite this

Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line : Evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells. / Hsieh, Chin Hsuan; Hsiung, Shih Chieh; Yeh, Chi Tai; Yen, Chih Feng; Chou, Yah Huei Wu; Lei, Wei Yi; Pang, See Tong; Chuang, Cheng Keng; Liao, Shuen Kuei.

In: Oncotarget, Vol. 8, No. 9, 01.01.2017, p. 15593-15609.

Research output: Contribution to journalArticle

Hsieh, Chin Hsuan ; Hsiung, Shih Chieh ; Yeh, Chi Tai ; Yen, Chih Feng ; Chou, Yah Huei Wu ; Lei, Wei Yi ; Pang, See Tong ; Chuang, Cheng Keng ; Liao, Shuen Kuei. / Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line : Evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells. In: Oncotarget. 2017 ; Vol. 8, No. 9. pp. 15593-15609.
@article{a658e5e374dc4dfd9dfe9023b32f060f,
title = "Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line: Evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells",
abstract = "Epithelioid and fibroblastoid subsets coexist in the human sarcomatoid renal cell carcinoma (sRCC) cell line, RCC52, according to previous clonal studies. Herein, using monoclonal antibodies to CD44 and CD24 markers, we identified and isolated these two populations, and showed that CD44bright/CD24dim and CD44bright/CD24bright phenotypes correspond to epithelioid and fibroblastoid subsets, respectively. Both sorted subsets displayed different levels of tumorigenicity in xenotransplantation, indicating that each harbored its own cancer stem cells (CSCs). The CD44bright/CD24bright subset, associated with higher expression of MMP-7, -8 and TIMP-1 transcripts, showed greater migratory/invasive potential than the CD44bright/CD24dim subset, which was associated with higher expression of MMP-2, -9 and TIMP-2 transcripts. Both subsets differentially expressed stemness gene products c-Myc, Oct4A, Notch1, Notch2 and Notch3, and the RCC stem cell marker, CD105 in 4-5{\%} of RCC52 cells. These results suggest the presence of CSCs in both sRCC subsets for the first time and should therefore be considered potential therapeutic targets for this aggressive malignancy.",
keywords = "Cancer stem cells, CD24, CD44, Epithelioid and fibroblastoid subsets, Sarcomatoid renal cell carcinoma",
author = "Hsieh, {Chin Hsuan} and Hsiung, {Shih Chieh} and Yeh, {Chi Tai} and Yen, {Chih Feng} and Chou, {Yah Huei Wu} and Lei, {Wei Yi} and Pang, {See Tong} and Chuang, {Cheng Keng} and Liao, {Shuen Kuei}",
year = "2017",
month = "1",
day = "1",
doi = "10.18632/oncotarget.14777",
language = "English",
volume = "8",
pages = "15593--15609",
journal = "Oncotarget",
issn = "1949-2553",
publisher = "Impact Journals LLC",
number = "9",

}

TY - JOUR

T1 - Differential expression of CD44 and CD24 markers discriminates the epitheliod from the fibroblastoid subset in a sarcomatoid renal carcinoma cell line

T2 - Evidence suggesting the existence of cancer stem cells in both subsets as studied with sorted cells

AU - Hsieh, Chin Hsuan

AU - Hsiung, Shih Chieh

AU - Yeh, Chi Tai

AU - Yen, Chih Feng

AU - Chou, Yah Huei Wu

AU - Lei, Wei Yi

AU - Pang, See Tong

AU - Chuang, Cheng Keng

AU - Liao, Shuen Kuei

PY - 2017/1/1

Y1 - 2017/1/1

N2 - Epithelioid and fibroblastoid subsets coexist in the human sarcomatoid renal cell carcinoma (sRCC) cell line, RCC52, according to previous clonal studies. Herein, using monoclonal antibodies to CD44 and CD24 markers, we identified and isolated these two populations, and showed that CD44bright/CD24dim and CD44bright/CD24bright phenotypes correspond to epithelioid and fibroblastoid subsets, respectively. Both sorted subsets displayed different levels of tumorigenicity in xenotransplantation, indicating that each harbored its own cancer stem cells (CSCs). The CD44bright/CD24bright subset, associated with higher expression of MMP-7, -8 and TIMP-1 transcripts, showed greater migratory/invasive potential than the CD44bright/CD24dim subset, which was associated with higher expression of MMP-2, -9 and TIMP-2 transcripts. Both subsets differentially expressed stemness gene products c-Myc, Oct4A, Notch1, Notch2 and Notch3, and the RCC stem cell marker, CD105 in 4-5% of RCC52 cells. These results suggest the presence of CSCs in both sRCC subsets for the first time and should therefore be considered potential therapeutic targets for this aggressive malignancy.

AB - Epithelioid and fibroblastoid subsets coexist in the human sarcomatoid renal cell carcinoma (sRCC) cell line, RCC52, according to previous clonal studies. Herein, using monoclonal antibodies to CD44 and CD24 markers, we identified and isolated these two populations, and showed that CD44bright/CD24dim and CD44bright/CD24bright phenotypes correspond to epithelioid and fibroblastoid subsets, respectively. Both sorted subsets displayed different levels of tumorigenicity in xenotransplantation, indicating that each harbored its own cancer stem cells (CSCs). The CD44bright/CD24bright subset, associated with higher expression of MMP-7, -8 and TIMP-1 transcripts, showed greater migratory/invasive potential than the CD44bright/CD24dim subset, which was associated with higher expression of MMP-2, -9 and TIMP-2 transcripts. Both subsets differentially expressed stemness gene products c-Myc, Oct4A, Notch1, Notch2 and Notch3, and the RCC stem cell marker, CD105 in 4-5% of RCC52 cells. These results suggest the presence of CSCs in both sRCC subsets for the first time and should therefore be considered potential therapeutic targets for this aggressive malignancy.

KW - Cancer stem cells

KW - CD24

KW - CD44

KW - Epithelioid and fibroblastoid subsets

KW - Sarcomatoid renal cell carcinoma

UR - http://www.scopus.com/inward/record.url?scp=85014125077&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85014125077&partnerID=8YFLogxK

U2 - 10.18632/oncotarget.14777

DO - 10.18632/oncotarget.14777

M3 - Article

C2 - 28121626

AN - SCOPUS:85014125077

VL - 8

SP - 15593

EP - 15609

JO - Oncotarget

JF - Oncotarget

SN - 1949-2553

IS - 9

ER -