Different sham procedures for rats in traumatic brain injury experiments induce corresponding increases in levels of trauma markers

John Wu, Kai Yun Chen, Yo Wen Yo, Song Wei Huang, Hsiu Ming Shih, Wen Ta Chiu, Yung Hsiao Chiang, Chia Yang Shiau

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Background: In traumatic brain injury animal models, sham or naïve control groups are often used for the analysis of injured animals; however, the existence and/or significance of differences in the control groups has yet to be studied. In addition, recent controversies regarding the decompressive craniectomy trial in which decompressive craniectomies in patients with severe traumatic brain injury and refractory increased intracranial pressure remains unsettled. Although the report demonstrated that the procedure may result in less favorable long-term outcomes despite the decrease in intracranial pressure and shorter length of intensive care unit stay, the study has been criticized, and the debate is still inconclusive partly because of a lack of mechanistic explanation. We have recently discovered epithelial and endothelial tyrosine kinase (Etk) to exhibit upregulation after traumatic neural injury and will compare the effects of craniectomy procedure with those of other procedures inducing different levels of severity. Materials and methods: Four groups of rats receiving different procedures (controlled cortical impact, craniectomy, bicortical drilling, and unicortical drilling [UD]) were compared. Polymerase chain reaction, Western blot analysis, and immunoflorescence staining of Etk, S100, and glial fibrillary acidic protein levels were used to analyze the results and compare the different groups. Results: Etk upregulation was statistically significant between craniectomy and UD groups. The level of change for glial fibrillary acidic protein and S100 was only significant when cortex was impacted. Conclusions: UD may be preferable as a sham control procedure over craniectomy or bicortical drilling. Increases in the expression of Etk in the craniectomy group suggest a possible mechanism by which unfavorable outcome occurs in patients receiving craniectomy procedures.

Original languageEnglish
Pages (from-to)138-144
Number of pages7
JournalJournal of Surgical Research
Volume179
Issue number1
DOIs
Publication statusPublished - Jan 2013

Fingerprint

Wounds and Injuries
Protein-Tyrosine Kinases
Decompressive Craniectomy
Glial Fibrillary Acidic Protein
Intracranial Pressure
Up-Regulation
Control Groups
Traumatic Brain Injury
Intensive Care Units
Animal Models
Western Blotting
Staining and Labeling
Polymerase Chain Reaction

Keywords

  • Controlled cortical impact
  • Craniectomy
  • Marker
  • Sham
  • Traumatic brain injury

ASJC Scopus subject areas

  • Surgery

Cite this

Different sham procedures for rats in traumatic brain injury experiments induce corresponding increases in levels of trauma markers. / Wu, John; Chen, Kai Yun; Yo, Yo Wen; Huang, Song Wei; Shih, Hsiu Ming; Chiu, Wen Ta; Chiang, Yung Hsiao; Shiau, Chia Yang.

In: Journal of Surgical Research, Vol. 179, No. 1, 01.2013, p. 138-144.

Research output: Contribution to journalArticle

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abstract = "Background: In traumatic brain injury animal models, sham or na{\"i}ve control groups are often used for the analysis of injured animals; however, the existence and/or significance of differences in the control groups has yet to be studied. In addition, recent controversies regarding the decompressive craniectomy trial in which decompressive craniectomies in patients with severe traumatic brain injury and refractory increased intracranial pressure remains unsettled. Although the report demonstrated that the procedure may result in less favorable long-term outcomes despite the decrease in intracranial pressure and shorter length of intensive care unit stay, the study has been criticized, and the debate is still inconclusive partly because of a lack of mechanistic explanation. We have recently discovered epithelial and endothelial tyrosine kinase (Etk) to exhibit upregulation after traumatic neural injury and will compare the effects of craniectomy procedure with those of other procedures inducing different levels of severity. Materials and methods: Four groups of rats receiving different procedures (controlled cortical impact, craniectomy, bicortical drilling, and unicortical drilling [UD]) were compared. Polymerase chain reaction, Western blot analysis, and immunoflorescence staining of Etk, S100, and glial fibrillary acidic protein levels were used to analyze the results and compare the different groups. Results: Etk upregulation was statistically significant between craniectomy and UD groups. The level of change for glial fibrillary acidic protein and S100 was only significant when cortex was impacted. Conclusions: UD may be preferable as a sham control procedure over craniectomy or bicortical drilling. Increases in the expression of Etk in the craniectomy group suggest a possible mechanism by which unfavorable outcome occurs in patients receiving craniectomy procedures.",
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