Different sample preparation and detection methods for normal and lung cancer urinary proteome analysis.

Supachok Sinchaikul, Payungsak Tantipaiboonwong, Supawadee Sriyam, Ching Tzao, Suree Phutrakul, Shui Tein Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The urinary proteome is known to be a valuable field of study related to human physiological functions because many components in urine provide an alternative to blood plasma as a potential source of disease biomarkers useful in clinical diagnosis and therapeutic application. Due to the variability and complexity of urine, sample preparation is very important for decreasing the dynamic range of components and isolating specific urinary proteins prior to analysis. We discuss many useful sample preparation methods in this chapter, including those of lung cancer urine samples. In addition, protein detection methods are also crucial in visualizing protein profiles and for quantification of protein content in urine samples from both normal donor and lung cancer patients. This chapter also provides alternative choices of urine sample preparation and detection methods for selective use in urinary proteome analysis and for identifying urinary protein markers in lung cancer and other diseases.

Original languageEnglish
Pages (from-to)65-88
Number of pages24
JournalMethods in molecular biology (Clifton, N.J.)
Volume641
DOIs
Publication statusPublished - 2010
Externally publishedYes

Fingerprint

Proteome
Lung Neoplasms
Urine
Proteins
Biomarkers
Tissue Donors

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Different sample preparation and detection methods for normal and lung cancer urinary proteome analysis. / Sinchaikul, Supachok; Tantipaiboonwong, Payungsak; Sriyam, Supawadee; Tzao, Ching; Phutrakul, Suree; Chen, Shui Tein.

In: Methods in molecular biology (Clifton, N.J.), Vol. 641, 2010, p. 65-88.

Research output: Contribution to journalArticle

Sinchaikul, Supachok ; Tantipaiboonwong, Payungsak ; Sriyam, Supawadee ; Tzao, Ching ; Phutrakul, Suree ; Chen, Shui Tein. / Different sample preparation and detection methods for normal and lung cancer urinary proteome analysis. In: Methods in molecular biology (Clifton, N.J.). 2010 ; Vol. 641. pp. 65-88.
@article{e2167cdb1519425a960eae63a920b591,
title = "Different sample preparation and detection methods for normal and lung cancer urinary proteome analysis.",
abstract = "The urinary proteome is known to be a valuable field of study related to human physiological functions because many components in urine provide an alternative to blood plasma as a potential source of disease biomarkers useful in clinical diagnosis and therapeutic application. Due to the variability and complexity of urine, sample preparation is very important for decreasing the dynamic range of components and isolating specific urinary proteins prior to analysis. We discuss many useful sample preparation methods in this chapter, including those of lung cancer urine samples. In addition, protein detection methods are also crucial in visualizing protein profiles and for quantification of protein content in urine samples from both normal donor and lung cancer patients. This chapter also provides alternative choices of urine sample preparation and detection methods for selective use in urinary proteome analysis and for identifying urinary protein markers in lung cancer and other diseases.",
author = "Supachok Sinchaikul and Payungsak Tantipaiboonwong and Supawadee Sriyam and Ching Tzao and Suree Phutrakul and Chen, {Shui Tein}",
year = "2010",
doi = "10.1007/978-1-60761-711-2_5",
language = "English",
volume = "641",
pages = "65--88",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

TY - JOUR

T1 - Different sample preparation and detection methods for normal and lung cancer urinary proteome analysis.

AU - Sinchaikul, Supachok

AU - Tantipaiboonwong, Payungsak

AU - Sriyam, Supawadee

AU - Tzao, Ching

AU - Phutrakul, Suree

AU - Chen, Shui Tein

PY - 2010

Y1 - 2010

N2 - The urinary proteome is known to be a valuable field of study related to human physiological functions because many components in urine provide an alternative to blood plasma as a potential source of disease biomarkers useful in clinical diagnosis and therapeutic application. Due to the variability and complexity of urine, sample preparation is very important for decreasing the dynamic range of components and isolating specific urinary proteins prior to analysis. We discuss many useful sample preparation methods in this chapter, including those of lung cancer urine samples. In addition, protein detection methods are also crucial in visualizing protein profiles and for quantification of protein content in urine samples from both normal donor and lung cancer patients. This chapter also provides alternative choices of urine sample preparation and detection methods for selective use in urinary proteome analysis and for identifying urinary protein markers in lung cancer and other diseases.

AB - The urinary proteome is known to be a valuable field of study related to human physiological functions because many components in urine provide an alternative to blood plasma as a potential source of disease biomarkers useful in clinical diagnosis and therapeutic application. Due to the variability and complexity of urine, sample preparation is very important for decreasing the dynamic range of components and isolating specific urinary proteins prior to analysis. We discuss many useful sample preparation methods in this chapter, including those of lung cancer urine samples. In addition, protein detection methods are also crucial in visualizing protein profiles and for quantification of protein content in urine samples from both normal donor and lung cancer patients. This chapter also provides alternative choices of urine sample preparation and detection methods for selective use in urinary proteome analysis and for identifying urinary protein markers in lung cancer and other diseases.

UR - http://www.scopus.com/inward/record.url?scp=77955479939&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77955479939&partnerID=8YFLogxK

U2 - 10.1007/978-1-60761-711-2_5

DO - 10.1007/978-1-60761-711-2_5

M3 - Article

C2 - 20407942

AN - SCOPUS:77955479939

VL - 641

SP - 65

EP - 88

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -